NRP2 (neuropilin-2), one of the 2 members of the neuropilin family, is a single-pass type I transmembrane glycoprotein.  Both members of  neuropilins contain a large N-terminal extracellular region consisting of 2 complement-binding (CUB),  2 coagulation factor V/VIII (F5/8 type C), and 1 meprin (MAM) domains in addition to a transmembrane domain and a small cytoplasmic tail. These specific domains allow neuropilins to participate in several different types of signaling pathways that control cell survival, migration, and attraction. Neuropilins bind many ligands and various types of co-receptors including vascular endothelial growth factor (VEGF) and semaphoring (SEMA) family members.  The tandem CUB domains mediate the binding to SEMA, while the tandem F5/8 type C domains are responsible for heparin and VEGF binding. NRP2 is implicated in cardiovascular development, axon guidance, and tumorigenesis. NPR2 is a high affinity receptor for SEMA3C, SEMA3F, VEGF-165 and VEGF-145 isoforms of VEGF, and the PLGF-2 isoform of PGF.

NRP2 (neuropilin-2), one of the 2 members of the neuropilin family, is a single-pass type I transmembrane glycoprotein.  Both members of  neuropilins contain a large N-terminal extracellular region consisting of 2 complement-binding (CUB),  2 coagulation factor V/VIII (F5/8 type C), and 1 meprin (MAM) domains in addition to a transmembrane domain and a small cytoplasmic tail. These specific domains allow neuropilins to participate in several different types of signaling pathways that control cell survival, migration, and attraction. Neuropilins bind many ligands and various types of co-receptors including vascular endothelial growth factor (VEGF) and semaphoring (SEMA) family members.  The tandem CUB domains mediate the binding to SEMA, while the tandem F5/8 type C domains are responsible for heparin and VEGF binding. NRP2 is implicated in cardiovascular development, axon guidance, and tumorigenesis. NPR2 is a high affinity receptor for SEMA3C, SEMA3F, VEGF-165 and VEGF-145 isoforms of VEGF, and the PLGF-2 isoform of PGF.

 

Product Details

 

Gene Symbol: NRP2; NP2; NPN2; PRO2714; VEGF165R2; neuropilin-2; neuropilin2 

 

NCBI Gene ID: 8828

 

Uniprot Entry: O60462

 

Construct Details: The recombinant human NRP2 ECD protein is expressed as an 1072-amino acid protein consisting of Arg21 -  Pro864 region of NRP2 and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions. NRP2 ECD contains 4 potential N-linked glycosylation sites.

 

Source: Human cells stably expressing NRP2-Fc and growing in chemical-defined media with no animal component or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60 

RGQPDPPCGG RLNSKDAGYI TSPGYPQDYP SHQNCEWIVY APEPNQKIVL NFNPHFEIEK  

        70         80         90        100        110        120 

HDCKYDFIEI RDGDSESADL LGKHCGNIAP PTIISSGSML YIKFTSDYAR QGAGFSLRYE  

       130        140        150        160        170        180 

IFKTGSEDCS KNFTSPNGTI ESPGFPEKYP HNLDCTFTIL AKPKMEIILQ FLIFDLEHDP  

       190        200        210        220        230        240 

LQVGEGDCKY DWLDIWDGIP HVGPLIGKYC GTKTPSELRS STGILSLTFH TDMAVAKDGF  

       250        260        270        280        290        300 

SARYYLVHQE PLENFQCNVP LGMESGRIAN EQISASSTYS DGRWTPQQSR LHGDDNGWTP  

       310        320        330        340        350        360 

NLDSNKEYLQ VDLRFLTMLT AIATQGAISR ETQNGYYVKS YKLEVSTNGE DWMVYRHGKN  

       370        380        390        400        410        420 

HKVFQANNDA TEVVLNKLHA PLLTRFVRIR PQTWHSGIAL RLELFGCRVT DAPCSNMLGM 

       430        440        450        460        470        480 

LSGLIADSQI SASSTQEYLW SPSAARLVSS RSGWFPRIPQ AQPGEEWLQV DLGTPKTVKG  

       490        500        510        520        530        540 

VIIQGARGGD SITAVEARAF VRKFKVSYSL NGKDWEYIQD PRTQQPKLFE GNMHYDTPDI  

       550        560        570        580        590        600 

RRFDPIPAQY VRVYPERWSP AGIGMRLEVL GCDWTDSKPT VETLGPTVKS EETTTPYPTE 

       610        620        630        640        650        660 

EEATECGENC SFEDDKDLQL PSGFNCNFDF LEEPCGWMYD HAKWLRTTWA SSSSPNDRTF  

       670        680        690        700        710        720 

PDDRNFLRLQ SDSQREGQYA RLISPPVHLP RSPVCMEFQY QATGGRGVAL QVVREASQES  

       730        740        750        760        770        780 

KLLWVIREDQ GGEWKHGRII LPSYDMEYQI VFEGVIGKGR SGEIAIDDIR ISTDVPLENC  

       790        800        810        820        830        840 

MEPISAFAGE NFKVDIPEIH EREGYEDEID DEYEVDWSNS SSATSGSGAP STDKEKSWLY  

       850        860        870        880        890        900 

TLDPSTGTHT CPPCPAPELL GGPSVFLFPP KPKDTLMISR TPEVTCVVVD VSHEDPEVKF  

       910        920        930        940        950        960 

NWYVDGVEVH NAKTKPREEQ YNSTYRVVSV LTVLHQDWLN GKEYKCKVSN KALPAPIEKT  

       970        980        990       1000       1010       1020 

ISKAKGQPRE PQVYTLPPSR EEMTKNQVSL TCLVKGFYPS DIAVEWESNG QPENNYKTTP  

      1030       1040       1050       1060       1070 

PVLDSDGSFF LYSKLTVDKS RWQQGNVFSC SVMHEALHNH YTQKSLSLSP GK 

 

  

M.W.: Calculated molecular mass (kDa): 120.6; Estimated by SDS-PAGE under reducing condition (kDa): 120-130

 

Calculated PI: 5.17

 

Calculated extinction coefficients (M-1 cm-1, at 280nm):  197570

  

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "S")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier & preservative free)

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Not available

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.