GPR75 (G-protein coupled receptor 75) is a 7 transmembrane receptor belonging to the G protein-coupled receptor 1 (GPCR1) family.  GPR75 is activated by the chemokine CCL5/RANTES. GPR75 is probably coupled to heterotrimeric Gq proteins and stimulates inositol trisphosphate production and calcium mobilization upon activation. Together with CCL5/RANTES, GPR75 may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. CCL5/RANTES may also regulate insulin secretion by pancreatic islet cells through activation of GPR75. 

GPR75 (G-protein coupled receptor 75) is a 7 transmembrane receptor belonging to the G protein-coupled receptor 1 (GPCR1) family.  GPR75 is activated by the chemokine CCL5/RANTES. GPR75 is probably coupled to heterotrimeric Gq proteins and stimulates inositol trisphosphate production and calcium mobilization upon activation. Together with CCL5/RANTES, GPR75 may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. CCL5/RANTES may also regulate insulin secretion by pancreatic islet cells through activation of GPR75. 

 

Product Details

 

Gene Symbol: GPR75; GPRchr2; WI31133

 

NCBI Gene ID: 10936

 

Uniprot Entry: O95800

 

Construct Details: Full length human GPR75 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1623 (bp)

 

Gene Insert Sequence:  

ATGAACTCAACAGGCCACCTTCAGGATGCCCCCAATGCCACCTCGCTCCATGTGCCTCACTCACAGGAAG

GAAACAGCACCTCTCTCCAGGAGGGTCTTCAGGATCTCATCCACACAGCCACCTTGGTGACCTGTACTTT

TCTACTGGCGGTCATCTTCTGCCTGGGTTCCTATGGCAACTTCATTGTCTTCTTGTCCTTCTTCGATCCA

GCCTTCAGGAAATTCAGAACCAACTTTGATTTCATGATCCTGAACCTGTCCTTCTGTGACCTCTTCATTT

GTGGAGTGACAGCCCCCATGTTCACCTTTGTGTTATTCTTCAGCTCAGCCAGTAGTATCCCGGATGCTTT

CTGCTTCACTTTCCATCTCACCAGTTCAGGCTTCATCATCATGTCTCTGAAGACAGTGGCAGTGATCGCC

CTGCACCGGCTCCGGATGGTGTTGGGGAAACAGCCTAATCGCACGGCCTCCTTTCCCTGCACCGTACTCC

TCACCCTGCTTCTCTGGGCCACCAGTTTCACCCTTGCCACCTTGGCTACCTTGAAAACCAGCAAGTCCCA

CCTCTGTCTTCCCATGTCCAGTCTGATTGCTGGAAAAGGGAAAGCCATTTTGTCTCTCTATGTGGTCGAC

TTCACCTTCTGTGTTGCTGTGGTCTCTGTCTCTTACATCATGATTGCTCAGACCCTGCGGAAGAACGCTC

AAGTCAGAAAGTGCCCCCCTGTAATCACAGTCGATGCTTCCAGACCACAGCCTTTCATGGGGGTCCCTGT

GCAGGGAGGTGGAGATCCCATCCAGTGTGCCATGCCGGCTCTGTATAGGAACCAGAATTACAACAAACTG

CAGCACGTTCAGACCCGTGGATATACCAAGAGTCCCAACCAACTGGTCACCCCTGCAGCAAGCCGACTCC

AGCTCGTATCAGCCATCAACCTCTCCACTGCCAAGGATTCCAAAGCCGTGGTCACCTGTGTGATCATTGT

GCTGTCAGTCCTGGTGTGCTGTCTTCCACTGGGGATTTCCTTGGTACAGGTGGTTCTCTCCAGCAATGGG

AGCTTCATTCTTTACCAGTTTGAATTGTTTGGATTTACTCTTATATTTTTCAAGTCAGGATTAAACCCTT

TTATATATTCTCGGAACAGTGCAGGGCTGAGAAGGAAAGTGCTCTGGTGCCTCCAATACATAGGCCTGGG

TTTTTTCTGCTGCAAACAAAAGACTCGACTTCGAGCCATGGGAAAAGGGAACCTCGAAGTCAACAGAAAC

AAATCCTCCCATCATGAAACAAACTCTGCCTACATGTTATCTCCAAAGCCACAGAAGAAATTTGTGGACC

AGGCTTGTGGCCCAAGTCATTCAAAAGAAAGTATGGTGAGTCCCAAGATCTCTGCTGGACATCAACACTG

TGGTCAGAGCAGCTCGACCCCCATCAACACTCGGATTGAACCTTACTACAGCATCTATAACAGCAGCCCT

TCCCAGGAGGAGAGCAGCCCATGTAACTTACAGCCAGTAAACTCTTTTGGATTTGCCAATTCATATATTG

CCATGCATTATCACACCACTAATGACTTAGTGCAGGAATATGACAGCACTTCAGCCAAGCAGATTCCAGT

CCCCTCCGTTTAA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

References

 

 

 

Additional supporting documents, including product datasheet, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.