GPR132 (G-protein coupled receptor 132) is a 7 transmembrane receptor belonging to the G protein-coupled receptor 1 (GPCR1) family.  GPR132 may be a receptor for oxidized free fatty acids derived from linoleic and arachidonic acids such as 9-hydroxyoctadecadienoic acid (9-HODE). GPR132 activates a G alpha protein, most likely G alpha(q). GPR132 may be involved in apoptosis. It functions at the G2/M checkpoint to delay mitosis and as a sensor that monitors the oxidative states and mediates appropriate cellular responses such as secretion of paracrine signals and attenuation of proliferation. GPR132 may mediate the accumulation of intracellular inositol phosphates at acidic pH through proton-sensing activity.

GPR132 (G-protein coupled receptor 132) is a 7 transmembrane receptor belonging to the G protein-coupled receptor 1 (GPCR1) family.  GPR132 may be a receptor for oxidized free fatty acids derived from linoleic and arachidonic acids such as 9-hydroxyoctadecadienoic acid (9-HODE). GPR132 activates a G alpha protein, most likely G alpha(q). GPR132 may be involved in apoptosis. It functions at the G2/M checkpoint to delay mitosis and as a sensor that monitors the oxidative states and mediates appropriate cellular responses such as secretion of paracrine signals and attenuation of proliferation. GPR132 may mediate the accumulation of intracellular inositol phosphates at acidic pH through proton-sensing activity.

 

Product Details

 

Gene Symbol: GPR132; G2A

 

NCBI Gene ID: 29933

 

Uniprot Entry: Q9UNW8

 

Construct Details: Full length human GPR132 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1143 (bp)

 

Gene Insert Sequence:  

ATGTGCCCAATGCTACTGAAAAACGGTTACAATGGAAACGCCACCCCAGTGACCACCACTGCCCCGTGGG

CCTCCCTGGGCCTCTCCGCCAAGACCTGCAACAACGTGTCCTTCGAAGAGAGCAGGATAGTCCTGGTCGT

GGTGTACAGCGCGGTGTGCACGCTGGGGGTGCCGGCCAACTGCCTGACTGCGTGGCTGGCGCTGCTGCAG

GTACTGCAGGGCAACGTGCTGGCCGTCTACCTGCTCTGCCTGGCACTCTGCGAGCTGCTGTACACAGGCA

CGCTGCCACTCTGGGTCATCTATATCCGCAACCAGCACCGCTGGACCCTAGGCCTGCTGGCCTGCAAGGT

GACCGCCTACATCTTCTTCTGCAACATCTACGTCAGCATCCTCTTCCTGTGCTGCATCTCCTGCGACCGC

TTCGTGGCCGTGGTGTACGCGCTGGAGAGTCGGGGCCGCCGCCGCCGGAGGACCGCCATCCTCATCTCCG

CCTGCATCTTCATCCTCGTCGGGATCGTTCACTACCCGGTGTTCCAGACGGAAGACAAGGAGACCTGCTT

TGACATGCTGCAGATGGACAGCAGGATTGCCGGGTACTACTACGCCAGGTTCACCGTTGGCTTTGCCATC

CCTCTCTCCATCATCGCCTTCACCAACCACCGGATTTTCAGGAGCATCAAGCAGAGCATGGGCTTAAGCG

CTGCCCAGAAGGCCAAGGTGAAGCACTCGGCCATCGCGGTGGTTGTCATCTTCCTAGTCTGCTTCGCCCC

GTACCACCTGGTTCTCCTCGTCAAAGCCGCTGCCTTTTCCTACTACAGAGGAGACAGGAACGCCATGTGC

GGCTTGGAGGAAAGGCTGTACACAGCCTCTGTGGTGTTTCTGTGCCTGTCCACGGTGAACGGCGTGGCTG

ACCCCATTATCTACGTGCTGGCCACGGACCATTCCCGCCAAGAAGTGTCCAGAATCCATAAGGGGTGGAA

AGAGTGGTCCATGAAGACAGACGTCACCAGGCTCACCCACAGCAGGGACACCGAGGAGCTGCAGTCGCCC

GTGGCCCTTGCAGACCACTACACCTTCTCCAGGCCCGTGCACCCACCAGGGTCACCATGCCCTGCAAAGA

GGCTGATTGAGGAGTCCTGCTGA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

References

 

 

 

Additional supporting documents, including product datasheet, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.