GPR68 (G-protein coupled receptor 68), also known as GPR12A and OGR1 (ovarian cancer G-protein coupled receptor 1), is a 7 transmembrane receptor belonging to the G protein-coupled receptor 1 (GPCR1) family.  GPR68 is a GPCR for sphingosylphosphorylcholine.  GPR68 acts as a proton-sensing receptor involved in pH homeostasis. GPR68 is inactive at pH 7.8 but active at pH 6.8. GPR68 represents an osteoblastic pH sensor regulating cell-mediated responses to acidosis in bone. It mediates its action by association with G proteins that stimulates inositol phosphate (IP) production or Ca2+ mobilization. GPR68 may function also as a metastasis suppressor gene in prostate cancer.  Mutations in the GPR68 gene are a cause of amelogenesis imperfecta, hypomaturation type, 2A6 (AI2A6), a defect of enamel formation.

GPR68 (G-protein coupled receptor 68), also known as GPR12A and OGR1 (ovarian cancer G-protein coupled receptor 1), is a 7 transmembrane receptor belonging to the G protein-coupled receptor 1 (GPCR1) family.  GPR68 is a GPCR for sphingosylphosphorylcholine.  GPR68 acts as a proton-sensing receptor involved in pH homeostasis. GPR68 is inactive at pH 7.8 but active at pH 6.8. GPR68 represents an osteoblastic pH sensor regulating cell-mediated responses to acidosis in bone. It mediates its action by association with G proteins that stimulates inositol phosphate (IP) production or Ca2+ mobilization. GPR68 may function also as a metastasis suppressor gene in prostate cancer.  Mutations in the GPR68 gene are a cause of amelogenesis imperfecta, hypomaturation type, 2A6 (AI2A6), a defect of enamel formation.

 

Product Details

 

Gene Symbol: GPR68; OGR1; AI2A6; GPR12A; ORG-1

 

NCBI Gene ID: 8111

 

Uniprot Entry: Q15743

 

Construct Details: Full length human GPR68 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1098 (bp)

 

Gene Insert Sequence:  

ATGGGGAACATCACTGCAGACAACTCCTCGATGAGCTGTACCATCGACCATACCATCCACCAGACGCTGG

CCCCGGTGGTCTATGTTACCGTGCTGGTGGTGGGCTTCCCGGCCAACTGCCTGTCCCTCTACTTCGGCTA

CCTGCAGATCAAGGCCCGGAACGAGCTGGGCGTGTACCTGTGCAACCTGACGGTGGCCGACCTCTTCTAC

ATCTGCTCGCTGCCCTTCTGGCTGCAGTACGTGCTGCAGCACGACAACTGGTCTCACGGCGACCTGTCCT

GCCAGGTGTGCGGCATCCTCCTGTACGAGAACATCTACATCAGCGTGGGCTTCCTCTGCTGCATCTCCGT

GGACCGCTACCTGGCTGTGGCCCATCCCTTCCGCTTCCACCAGTTCCGGACCCTGAAGGCGGCCGTCGGC

GTCAGCGTGGTCATCTGGGCCAAGGAGCTGCTGACCAGCATCTACTTCCTGATGCACGAGGAGGTCATCG

AGGACGAGAACCAGCACCGCGTGTGCTTTGAGCACTACCCCATCCAGGCATGGCAGCGCGCCATCAACTA

CTACCGCTTCCTGGTGGGCTTCCTCTTCCCCATCTGCCTGCTGCTGGCGTCCTACCAGGGCATCCTGCGC

GCCGTGCGCCGGAGCCACGGCACCCAGAAGAGCCGCAAGGACCAGATCCAGCGGCTGGTGCTCAGCACCG

TGGTCATCTTCCTGGCCTGCTTCCTGCCCTACCACGTGTTGCTGCTGGTGCGCAGCGTCTGGGAGGCCAG

CTGCGACTTCGCCAAGGGCGTTTTCAACGCCTACCACTTCTCCCTCCTGCTCACCAGCTTCAACTGCGTC

GCCGACCCCGTGCTCTACTGCTTCGTCAGCGAGACCACCCACCGGGACCTGGCCCGCCTCCGCGGGGCCT

GCCTGGCCTTCCTCACCTGCTCCAGGACCGGCCGGGCCAGGGAGGCCTACCCGCTGGGTGCCCCCGAGGC

CTCCGGGAAAAGCGGGGCCCAGGGTGAGGAGCCCGAGCTGTTGACCAAGCTCCACCCGGCCTTCCAGACC

CCTAACTCGCCAGGGTCGGGCGGGTTCCCCACGGGCAGGTTGGCCTAG

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

References

 

 

 

Additional supporting documents, including product datasheet, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.