CMKLR1 (chemerin chemokine-like receptor 1) is a 7-transmembrane protein of the G protein-coupled receptor 1 (GPCR1) family. CMKLR1 acts as a receptor for the chemoattractant adipokine chemerin/RARRES2 and for the omega-3 fatty acid derived molecule resolvin E1. Interaction with RARRES2 induces activation of intracellular signaling molecules, such as SKY, MAPK1/3 (ERK1/2), MAPK14/P38MAPK and PI3K leading to multifunctional effects, like, reduction of immune responses, enhancing of adipogenesis and angionesis. CMKLR1 may positively regulates adipogenesis and adipocyte metabolism. It may act as a co-receptor for several SIV strains as well as a primary HIV-1 strain.

CMKLR1 (chemerin chemokine-like receptor 1) is a 7-transmembrane protein of the G protein-coupled receptor 1 (GPCR1) family. CMKLR1 acts as a receptor for the chemoattractant adipokine chemerin/RARRES2 and for the omega-3 fatty acid derived molecule resolvin E1. Interaction with RARRES2 induces activation of intracellular signaling molecules, such as SKY, MAPK1/3 (ERK1/2), MAPK14/P38MAPK and PI3K leading to multifunctional effects, like, reduction of immune responses, enhancing of adipogenesis and angionesis. CMKLR1 may positively regulates adipogenesis and adipocyte metabolism. It may act as a co-receptor for several SIV strains as well as a primary HIV-1 strain.

 

Product Details

 

Gene Symbol: CMKLR1; CHEMR23; DEZ; RVER1; ChemR-23; CHEMERINR

 

NCBI Gene ID: 1240

 

Uniprot Entry: Q99788

 

Construct Details: Full length human CMKLR1 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without an antibiotic selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter +/- a selection marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1122 (bp)

 

Gene Insert Sequence:  

ATGAGAATGGAGGATGAAGATTACAACACTTCCATCAGTTACGGTGATGAATACCCTGATTATTTAGACT

CCATTGTGGTTTTGGAGGACTTATCCCCCTTGGAAGCCAGGGTGACCAGGATCTTCCTGGTGGTGGTCTA

CAGCATCGTCTGCTTCCTCGGGATTCTGGGCAATGGTCTGGTGATCATCATTGCCACCTTCAAGATGAAG

AAGACAGTGAACATGGTCTGGTTCCTCAACCTGGCAGTGGCAGATTTCCTGTTCAACGTCTTCCTCCCAA

TCCATATCACCTATGCCGCCATGGACTACCACTGGGTTTTCGGGACAGCCATGTGCAAGATCAGCAACTT

CCTTCTCATCCACAACATGTTCACCAGCGTCTTCCTGCTGACCATCATCAGCTCTGACCGCTGCATCTCT

GTGCTCCTCCCTGTCTGGTCCCAGAACCACCGCAGCGTTCGCCTGGCTTACATGGCCTGCATGGTCATCT

GGGTCCTGGCTTTCTTCTTGAGTTCCCCATCTCTCGTCTTCCGGGACACAGCCAACCTGCATGGGAAAAT

ATCCTGCTTCAACAACTTCAGCCTGTCCACACCTGGGTCTTCCTCGTGGCCCACTCACTCCCAAATGGAC

CCTGTGGGGTATAGCCGGCACATGGTGGTGACTGTCACCCGCTTCCTCTGTGGCTTCCTGGTCCCAGTCC

TCATCATCACAGCTTGCTACCTCACCATCGTGTGCAAACTGCAGCGCAACCGCCTGGCCAAGACCAAGAA

GCCCTTCAAGATTATTGTGACCATCATCATTACCTTCTTCCTCTGCTGGTGCCCCTACCACACACTCAAC

CTCCTAGAGCTCCACCACACTGCCATGCCTGGCTCTGTCTTCAGCCTGGGTTTGCCCCTGGCCACTGCCC

TTGCCATTGCCAACAGCTGCATGAACCCCATTCTGTATGTTTTCATGGGTCAGGACTTCAAGAAGTTCAA

GGTGGCCCTCTTCTCTCGCCTGGTCAATGCTCTAAGTGAAGATACAGGCCACTCTTCCTACCCCAGCCAT

AGAAGCTTTACCAAGATGTCATCAATGAATGAGAGGACTTCTATGAATGAGAGGGAGACCGGCATGCTTT

GA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.