Tie1 (Tyrosine kinase with Ig and EGF homology domains 1) and Tie2 comprise a receptor tyrosine kinase (RTK) subfamily with unique structural features: two immunoglobulin­like (Ig) domains flanking three epidermal growth factor (EGF)­like domains and followed by three fibronectin type III­like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. Tie1 and Tie2 are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis, and hematopoiesis. Tie2 is a receptor for the angiopoietin (ANG) family: ANG1, ANG2, and ANG3 (mouse)/ANG4 (human).  Tie1 may also act as an ANG receptor, possibly in complex with Tie2. Tie1 is an angiopoietin receptor and is an orphan RTK expressed almost exclusively in endothelial cells and required for normal embryonic vascular development. Tie-1 kinase has little to no activity. Tie1 plays an important role in the differentiation of endothelial cells and the maintenance of integrity of the endothelium. Tie1 may inhibit angiopoietin 1 signaling through the endothelial receptor tyrosine kinase Tie2 and ectodomain cleavage of the Tie1 relieves inhibition of Tie2.  Tie1 is expressed in epithelial tumours in breast, thyroid and gastric cancers. In addition Tie-1 has a pro-inflammatory property and may play a role in the endothelial inflammatory diseases such as atherosclerosis.

Tie1 (Tyrosine kinase with Ig and EGF homology domains 1) and Tie2 comprise a receptor tyrosine kinase (RTK) subfamily with unique structural features: two immunoglobulin­like (Ig) domains flanking three epidermal growth factor (EGF)­like domains and followed by three fibronectin type III­like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. Tie1 and Tie2 are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis, and hematopoiesis. Tie2 is a receptor for the angiopoietin (ANG) family: ANG1, ANG2, and ANG3 (mouse)/ANG4 (human).  Tie1 may also act as an ANG receptor, possibly in complex with Tie2. Tie1 is an angiopoietin receptor and is an orphan RTK expressed almost exclusively in endothelial cells and required for normal embryonic vascular development. Tie-1 kinase has little to no activity. Tie1 plays an important role in the differentiation of endothelial cells and the maintenance of integrity of the endothelium. Tie1 may inhibit angiopoietin 1 signaling through the endothelial receptor tyrosine kinase Tie2 and ectodomain cleavage of the Tie1 relieves inhibition of Tie2.  Tie1 is expressed in epithelial tumours in breast, thyroid and gastric cancers. In addition Tie-1 has a pro-inflammatory property and may play a role in the endothelial inflammatory diseases such as atherosclerosis.

 

Product Details

 

Gene Symbol: Tie1; TIE; JTK14

 

NCBI Gene ID: 7075

 

Uniprot Entry: P35590

 

Construct Details: The recombinant human Tie1-Fc fusion is expressed as a 967 amino acid protein consisting of Ala22 - Gln759 region of Tie1 and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions (see the gel image above).

 

Source: Human cells stably expressing human Tie1-Fc and growing in chemical-defined media with no animal components or antibiotics

 

Deduced Amino Acid Sequence:

AVDLTLLANLRLTDPQRFFLTCVSGEAGAGRGSDAWGPPLLLEKDDRIVRTPPGPPLRLAR

NGSHQVTLRGFSKPSDLVGVFSCVGGAGARRTRVIYVHNSPGAHLLPDKVTHTVNKGDTAV

LSARVHKEKQTDVIWKSNGSYFYTLDWHEAQDGRFLLQLPNVQPPSSGIYSATYLEASPLG

SAFFRLIVRGCGAGRWGPGCTKECPGCLHGGVCHDHDGECVCPPGFTGTRCEQACREGRFG

QSCQEQCPGISGCRGLTFCLPDPYGCSCGSGWRGSQCQEACAPGHFGADCRLQCQCQNGGT

CDRFSGCVCPSGWHGVHCEKSDRIPQILNMASELEFNLETMPRINCAAAGNPFPVRGSIEL

RKPDGTVLLSTKAIVEPEKTTAEFEVPRLVLADSGFWECRVSTSGGQDSRRFKVNVKVPPV

PLAAPRLLTKQSRQLVVSPLVSFSGDGPISTVRLHYRPQDSTMDWSTIVVDPSENVTLMNL

RPKTGYSVRVQLSRPGEGGEGAWGPPTLMTTDCPEPLLQPWLEGWHVEGTDRLRVSWSLPL

VPGPLVGDGFLLRLWDGTRGQERRENVSSPQARTALLTGLTPGTHYQLDVQLYHCTLLGPA

SPPAHVLLPPSGPPAPRHLHAQALSDSEIQLTWKHPEALPGPISKYVVEVQVAGGAGDPLW

IDVDRPEETSTIIRGLNASTRYLFRMRASIQGLGDWSNTVEESTLGNGLQAEGPVQESRAA

EEGLDQGSTGTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEV

KFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEK

TISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTP

PVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK

   

M.W.: Calculated molecular mass (kDa): 105.5; Estimated by SDS-PAGE under reducing condition (kDa): 110-120

 

Calculated PI: 6.64

 

Calculated extinction coefficients (M-1 cm-1, at 280nm): 143540

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "DTT:+")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier & preservative free).

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Interacts with Tie2 and inhibits angiopoietin/Tie2-mediated signaling activity.

 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks.

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. Mol. Cell. Biol. 12:1698 (1992)

2. Nature. 376: 70 (1995).

3. Mol. Cell 37:643 (2010).

4. Mol. Cell. Biol. 22: 1704 (2002).

5. J. Cell Biol. 169:239 (2005)

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: FCL0846-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.