KL (klotho) is a single-pass, type I transmembrane protein that belongs to a the Klotho subfamily of the glycosyl hydrolase 1 family. KL contains 2 glycosyl hydrolase 1 regions in the extracellular domain (ECD) and may have weak glycosidase activity towards glucuronylated steroids. However, KL lacks essential active site Glu residues at positions 239 and 872.  KL can be cleaved into Klotho peptide which may be an anti-aging circulating hormone to extend life span by inhibiting insulin/IGF1 signaling. KL is detected in cortical renal tubules and soluble peptide is present in serum and cerebrospinal fluid. KL is expressed in kidney, placenta, small intestine and prostate. It is down-regulated in renal cell carcinomas, hepatocellular carcinomas, and in chronic renal failure (CRF) kidney.  KL is involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D. KL plays important roles in degenerative processes (e.g., arteriosclerosis, osteoporosis, and skin atrophy) observed in CRF. Mutations within the KL gene are associated with aging and bone loss. 

KL (klotho) is a single-pass, type I transmembrane protein that belongs to a the Klotho subfamily of the glycosyl hydrolase 1 family. KL contains 2 glycosyl hydrolase 1 regions in the extracellular domain (ECD) and may have weak glycosidase activity towards glucuronylated steroids. However, KL lacks essential active site Glu residues at positions 239 and 872.  KL can be cleaved into Klotho peptide which may be an anti-aging circulating hormone to extend life span by inhibiting insulin/IGF1 signaling. KL is detected in cortical renal tubules and soluble peptide is present in serum and cerebrospinal fluid. KL is expressed in kidney, placenta, small intestine and prostate. It is down-regulated in renal cell carcinomas, hepatocellular carcinomas, and in chronic renal failure (CRF) kidney.  KL is involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D. KL plays important roles in degenerative processes (e.g., arteriosclerosis, osteoporosis, and skin atrophy) observed in CRF. Mutations within the KL gene are associated with aging and bone loss. 

 

Product Details

 

Gene Symbol: KL; Klotho

 

NCBI Gene ID: 9365

 

Uniprot Entry: Q9UEF7

 

Construct Details: Full length human KL/Klotho gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without an antibiotic selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter +/- a selection marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 3039 (bp)

 

Gene Insert Sequence:  

ATGCCCGCCAGCGCCCCGCCGCGCCGCCCGCGGCCGCCGCCGCCGTCGCTGTCGCTGCTGCTGGTGCTGC

TGGGCCTGGGCGGCCGCCGCCTGCGTGCGGAGCCGGGCGACGGCGCGCAGACCTGGGCCCGTTTCTCGCG

GCCTCCTGCCCCCGAGGCCGCGGGCCTCTTCCAGGGCACCTTCCCCGACGGCTTCCTCTGGGCCGTGGGC

AGCGCCGCCTACCAGACCGAGGGCGGCTGGCAGCAGCACGGCAAGGGTGCGTCCATCTGGGATACGTTCA

CCCACCACCCCCTGGCACCCCCGGGAGACTCCCGGAACGCCAGTCTGCCGTTGGGCGCCCCGTCGCCGCT

GCAGCCCGCCACCGGGGACGTAGCCAGCGACAGCTACAACAACGTCTTCCGCGACACGGAGGCGCTGCGC

GAGCTCGGGGTCACTCACTACCGCTTCTCCATCTCGTGGGCGCGAGTGCTCCCCAATGGCAGCGCGGGCG

TCCCCAACCGCGAGGGGCTGCGCTACTACCGGCGCCTGCTGGAGCGGCTGCGGGAGCTGGGCGTGCAGCC

CGTGGTCACCCTGTACCACTGGGACCTGCCCCAGCGCCTGCAGGACGCCTACGGCGGCTGGGCCAACCGC

GCCCTGGCCGACCACTTCAGGGATTACGCGGAGCTCTGCTTCCGCCACTTCGGCGGTCAGGTCAAGTACT

GGATCACCATCGACAACCCCTACGTGGTGGCCTGGCACGGCTACGCCACCGGGCGCCTGGCCCCCGGCAT

CCGGGGCAGCCCGCGGCTCGGGTACCTGGTGGCGCACAACCTCCTCCTGGCTCATGCCAAAGTCTGGCAT

CTCTACAATACTTCTTTCCGTCCCACTCAGGGAGGTCAGGTGTCCATTGCCCTAAGCTCTCACTGGATCA

ATCCTCGAAGAATGACCGACCACAGCATCAAAGAATGTCAAAAATCTCTGGACTTTGTACTAGGTTGGTT

TGCCAAACCCGTATTTATTGATGGTGACTATCCCGAGAGCATGAAGAATAACCTTTCATCTATTCTGCCT

GATTTTACTGAATCTGAGAAAAAGTTCATCAAAGGAACTGCTGACTTTTTTGCTCTTTGCTTTGGACCCA

CCTTGAGTTTTCAACTTTTGGACCCTCACATGAAGTTCCGCCAATTGGAATCTCCCAACCTGAGGCAACT

GCTTTCCTGGATTGACCTTGAATTTAACCATCCTCAAATATTTATTGTGGAAAATGGCTGGTTTGTCTCA

GGGACCACCAAGAGAGATGATGCCAAATATATGTATTACCTCAAAAAGTTCATCATGGAAACCTTAAAAG

CCATCAAGCTGGATGGGGTGGATGTCATCGGGTATACCGCATGGTCCCTCATGGATGGTTTCGAGTGGCA

CAGAGGTTACAGCATCAGGCGTGGACTCTTCTATGTTGACTTTCTAAGCCAGGACAAGATGTTGTTGCCA

AAGTCTTCAGCCTTGTTCTACCAAAAGCTGATAGAGAAAAATGGCTTCCCTCCTTTACCTGAAAATCAGC

CCCTAGAAGGGACATTTCCCTGTGACTTTGCTTGGGGAGTTGTTGACAACTACATTCAAGTAGATACCAC

TCTGTCTCAGTTTACCGACCTGAATGTTTACCTGTGGGATGTCCACCACAGTAAAAGGCTTATTAAAGTG

GATGGGGTTGTGACCAAGAAGAGGAAATCCTACTGTGTTGACTTTGCTGCCATCCAGCCCCAGATCGCTT

TACTCCAGGAAATGCACGTTACACATTTTCGCTTCTCCCTGGACTGGGCCCTGATTCTCCCTCTGGGTAA

CCAGTCCCAGGTGAACCACACCATCCTGCAGTACTATCGCTGCATGGCCAGCGAGCTTGTCCGTGTCAAC

ATCACCCCAGTGGTGGCCCTGTGGCAGCCTATGGCCCCGAACCAAGGACTGCCGCGCCTCCTGGCCAGGC

AGGGCGCCTGGGAGAACCCCTACACTGCCCTGGCCTTTGCAGAGTATGCCCGACTGTGCTTTCAAGAGCT

CGGCCATCACGTCAAGCTTTGGATAACGATGAATGAGCCGTATACAAGGAATATGACATACAGTGCTGGC

CACAACCTTCTGAAGGCCCATGCCCTGGCTTGGCATGTGTACAATGAAAAGTTTAGGCATGCTCAGAATG

GGAAAATATCCATAGCCTTGCAGGCTGATTGGATAGAACCTGCCTGCCCTTTCTCCCAAAAGGACAAAGA

GGTGGCTGAGAGAGTTTTGGAATTTGACATTGGCTGGCTGGCTGAGCCCATTTTCGGCTCTGGAGATTAT

CCATGGGTGATGAGGGACTGGCTGAACCAAAGAAACAATTTTCTTCTTCCTTATTTCACTGAAGATGAAA

AAAAGCTAATCCAGGGTACCTTTGACTTTTTGGCTTTAAGCCATTATACCACCATCCTTGTAGACTCAGA

AAAAGAAGATCCAATAAAATACAATGATTACCTAGAAGTGCAAGAAATGACCGACATCACGTGGCTCAAC

TCCCCCAGTCAGGTGGCGGTAGTGCCCTGGGGGTTGCGCAAAGTGCTGAACTGGCTGAAGTTCAAGTACG

GAGACCTCCCCATGTACATAATATCCAATGGAATCGATGACGGGCTGCATGCTGAGGACGACCAGCTGAG

GGTGTATTATATGCAGAATTACATAAACGAAGCTCTCAAAGCCCACATACTGGATGGTATCAATCTTTGC

GGATACTTTGCTTATTCGTTTAACGACCGCACAGCTCCGAGGTTTGGCCTCTATCGTTATGCTGCAGATC

AGTTTGAGCCCAAGGCATCCATGAAACATTACAGGAAAATTATTGACAGCAATGGTTTCCCGGGCCCAGA

AACTCTGGAAAGATTTTGTCCAGAAGAATTCACCGTGTGTACTGAGTGCAGTTTTTTTCACACCCGAAAG

TCTTTACTGGCTTTCATAGCTTTTCTATTTTTTGCTTCTATTATTTCTCTCTCCCTTATATTTTACTACT

CGAAGAAAGGCAGAAGAAGTTACAAATAG

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.