CEACAM20 (carcinoembryonic antigen (CEA)-related cell adhesion molecule 20) is a single pass type I transmembrane protein of the CEA family of the immunoglobulin superfamily (IgSF). CEA family members are either transmembrane or GPI­-anchored within two subgroups: the CEACAMs and the pregnancy-specific glycoproteins that are used by several bacterial pathogens to bind and invade host cells.  Members of the CEA family consist of a single N domain, with structural similarity to the Ig variable (IgV) domains, followed by a variable number of Ig constant-like 1 (IgC1) and/or 2 (IgC2) domains.  In human, 7 CEACAMs have been identified including 3 transmembrane proteins (CEACAM1, 3, 4) and 4 GPI-anchored molecules (CEACAM5-8). Human CEACAM20 contains 4 C2-type Ig-like (IgC2) motifs in the N-terminal 420 aa extracellular domain (ECD), followed by a transmembrane domain (TM) and a 114 aa cytoplasmic tail. CEACAM20 enhances production of the cytokine CXCL8/IL-8 via the NF-kappaB pathway and may thus have a role in the intestinal immune response.

CEACAM20 (carcinoembryonic antigen (CEA)-related cell adhesion molecule 20) is a single pass type I transmembrane protein of the CEA family of the immunoglobulin superfamily (IgSF). CEA family members are either transmembrane or GPI­-anchored within two subgroups: the CEACAMs and the pregnancy-specific glycoproteins that are used by several bacterial pathogens to bind and invade host cells.  Members of the CEA family consist of a single N domain, with structural similarity to the Ig variable (IgV) domains, followed by a variable number of Ig constant-like 1 (IgC1) and/or 2 (IgC2) domains.  In human, 7 CEACAMs have been identified including 3 transmembrane proteins (CEACAM1, 3, 4) and 4 GPI-anchored molecules (CEACAM5-8). Human CEACAM20 contains 4 C2-type Ig-like (IgC2) motifs in the N-terminal 420 aa extracellular domain (ECD), followed by a transmembrane domain (TM) and a 114 aa cytoplasmic tail. CEACAM20 enhances production of the cytokine CXCL8/IL-8 via the NF-kappaB pathway and may thus have a role in the intestinal immune response.

 

Product Details

 

Gene Symbol: CEACAM20

 

NCBI Gene ID: 125931

 

Uniprot Entry: Q6UY09

 

Construct Details: Full length human CEACAM20 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1755 (bp)

 

Gene Insert Sequence:  

ATGGGGCCTGCTGACTCATGGGGACACCACTGGATGGGAATCCTGCTTTCAGCCTCGCTTTGTACCGTAT

GGAGTCCTCCAGCTGCAGCCCAGCTCACCCTCAATGCCAACCCACTTGATGCCACCCAAAGTGAGGATGT

TGTTCTGCCTGTGTTTGGGACCCCCAGGACACCCCAGATTCATGGCAGATCCAGAGAGCTGGCCAAACCC

TCCATTGCAGTCAGCCCAGGCACTGCCATAGAGCAGAAGGACATGGTGACCTTCTACTGCACCACTAAGG

ACGTCAACATTACCATCCACTGGGTTTCCAACAACCTCTCCATTGTGTTCCATGAGCGCATGCAGCTGTC

CAAGGATGGCAAGATCCTCACCATTCTCATTGTCCAGCGGGAGGACTCAGGGACTTACCAATGTGAAGCT

CGAGATGCCCTTCTGAGCCAGAGGAGCGACCCCATCTTCCTGGATGTGAAGTATGGTCCTGATCCTGTTG

AAATCAAATTGGAGTCTGGTGTTGCCAGTGGGGAGGTGGTTGAGGTGATGGAGGGCTCCAGCATGACCTT

CTTAGCGGAAACAAAGTCTCACCCACCCTGTGCCTATACTTGGTTTCTCCTTGACTCCATTCTGTCTCAC

ACCACGAGAACATTCACCATCCATGCTGTGTCCAGAGAACATGAGGGCCTGTACAGGTGCTTGGTGTCCA

ACAGTGCCACCCACCTGTCCAGCCTGGGTACTCTGAAGGTCCGAGTACTTGAAACACTGACCATGCCTCA

AGTCGTGCCTTCAAGCCTGAACCTTGTGGAGAATGCTAGGTCTGTGGACCTGACCTGCCAAACCGTCAAT

CAGAGTGTGAATGTCCAGTGGTTCCTAAGTGGCCAGCCCCTCCTGCCCAGTGAGCACCTGCAGCTGTCAG

CTGACAACAGGACCCTAATCATCCATGGCCTCCAGCGGAATGACACCGGGCCCTATGCCTGTGAGGTCTG

GAACTGGGGCAGCCGGGCCCGGAGTGAGCCCCTTGAGCTGACCATCAACTATGGTCCTGACCAAGTGCAC

ATCACCAGGGAGTCGGCATCTGAGATGATCAGCACCATAGAGGCAGAGCTCAACTCCAGCCTGACCCTGC

AGTGTTGGGCCGAGTCCAAGCCAGGTGCTGAGTATCGCTGGACTCTTGAACACTCCACCGGGGAGCACCT

GGGTGAGCAGCTGATTATCAGGGCTCTGACCTGGGAACACGACGGGATCTACAACTGCACAGCCTCCAAC

TCTCTCACTGGCCTGGCCCGCTCCACTTCAGTCCTGGTCAAGGTGGTAGGTCCCCAGTCCTCCTCCCTGT

CCTCAGGGGCCATCGCTGGTATTGTCATCGGGATCCTGGCTGTCATTGCTGTGGCCTCAGAACTGGGCTA

TTTTCTCTGCATCAGAAATGCCAGACGGCCCTCAAGGAAAACAACAGAGGACCCCAGTCATGAGACCTCA

CAACCCATCCCGAAGGAGGAGCACCCCACAGAGCCCAGTTCCGAAAGCCTGAGTCCTGAGTATCGCAATA

TATCCCAGCTTCAGGGACGGATCAGAGTCGAACTGACGAAGCTGCCTTCAGCAAGCCGTAGAGGCAATTC

TTTCAGCCCCTGGAAGCCACCACCCAAACCTCTGATGCCCCCACTCAGATTGGTCTCCACTGTGCCAAAA

AACATGGAGTCAATCTATGAGGAGCTTGTGAATCCAGAGCCCAACACTTACATCCAAATCAACCCCTCCG

TCTAA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.