C1orf101 (chromosome 1 open reading frame 101) also known as CATSPERE (Cation channel sperm-associated protein subunit epsilon) is a single-pass type I transmembrane protein.  C1orf101 may be an auxiliary component of the CatSper complex, a complex involved in sperm cell hyperactivation. Sperm cell hyperactivation is needed for sperm motility which is essential late in the preparation of sperm for fertilization.

C1orf101 (chromosome 1 open reading frame 101) also known as CATSPERE (Cation channel sperm-associated protein subunit epsilon) is a single-pass type I transmembrane protein.  C1orf101 may be an auxiliary component of the CatSper complex, a complex involved in sperm cell hyperactivation. Sperm cell hyperactivation is needed for sperm motility which is essential late in the preparation of sperm for fertilization.

 

Product Details

 

Gene Symbol: C1orf101; CATSPERE

 

NCBI Gene ID: 257044

 

Uniprot Entry: Q5SY80

 

Construct Details: Full length human C1orf101 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without an antibiotic selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter +/- a selection marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 2856 (bp)

 

Gene Insert Sequence:  

ATGTCAGCCCGGGAAGTGGCCGTGCTGCTGCTGTGGCTGAGCTGCTATGGCTCCGCCCTTTGGAGGTATT

CCACTAACAGCCCAAACTATCGCATTTTTAGTACCAGAAGTACTATTAAGTTAGAGTATGAAGGAACATT

ATTTACTGAGTGGAGTGTGCCAGAAACTTGTTTTGTGCTAAATAAAAGCTCACCCACGACAGAATTGCGT

TGTTCCTCACCTGGTGTTCACGCTATAAAACCAATTGTTACTGGCCCAGATGAAGAAGAACGCTATTTAT

TTGTGGAAAGTTCTCATACTTGCTTTCTGTGGTACTATAGAGTTAGACATTTCTTTAACAACTTTACCCA

GCTTATCACTGTGTGGGCATATGATCCAGAAAGTGCAGATCCTGATGAGTTGCTGGGGAATGCAGAAGAA

CCTTCAATAAATTCCATAGTACTCAGCACACAGATGGCCACATTGGGACAGAAGCCTGTCATACATACAG

TTCTGAAGAGAAAAGTTTATTCTTCAAATGAGAAAATGAGAAGGGGGACCTGGCGTATTGTAGTACCAAT

GACAAAAGATGATGCACTAAAGGAGATTAGAGGAAACCAAGTTACTTTTCAGGATTGCTTTATTGCAGAT

TTTCTTATTCTGTTGACTTTTCCTTTGTTGACCATACCTGAAATTCCTGGTTATTTACCAATCTCCTCAC

CACGTGGTAGTCAATTAATGGCTTCCTGGGATGCTTGTGTAGTTGCATCTGCTGTTTTGGTGACAGATAT

GGAGACCTTTCACACAACTGATTCATTCAAATCTTGGACCAGAATCAGAGTGCCTCCAGACATTCTGAGT

GATGATGAAAGACGGAGTGTGGCTCATGTGATCTTATCGCGGGATGGAATCGTTTTTCTTATAAATGGTG

TTCTTTACATAAAGAGTTTTCGTGGATTTATAAGACTGGGAGGAATTGTAAATCTTCCTGATGGTGGAAT

TACTGGCATTTCATCAAGAAAATGGTGTTGGGTCAATTATTTATTAAAGGCTAAAGGAAGAAGAAGCACC

TTTGCAGTCTGGACAGAAAATGAAATTTACCTCGGATCCATTCTTCTTAAGTTTGCCAGATTAGTAACTA

CCACAGAACTGAAAAACATCCTAAGTCTATCGGTGACTGCTACTCTGACCATAGACAGGGTTGAGTATAC

AGGACACCCTCTGGAGATTGCTGTGTTTTTAAATTATTGCACTGTATGTAACGTCACCAAAAAGATTTTC

TTAGTGATATATAATGAAGATACAAAACAGTGGGTTTCCCAAGACTTTACATTAGATGCCCCTATTGACA

GTGTTACCATGCCACATTTTACATTTTCAGCACTGCCAGGATTACTGCTATGGAACAAGCATAGTATCTA

CTATTGTTACCATAATTTCACCTTTACTGGGATTTTACAGACACCTGCAGGACATGGAAATCTATCAATG

CTATCAAATGACAGCATTATTCATGAAGTTTTCATAGATTATTATGGAGATATTTTGGTAAAAATGGAAA

ATAATGTAATATTTTATTCCAAGATTAATACTAGAGATGCAGTAAAGCTGCATTTATGGACAAATTACAC

AACAAGAGCATTCATTTTCTTAAGTACATCTGGTCAAACATATTTCCTGTATGCTTTGGATGATGGCACA

ATACAAATACAGGACTATCCCTTACATCTGGAAGCACAAAGTATAGCTTTCACAACAAAAGACAAATGCC

CATACATGGCATTTCATAACAATGTTGCTCATGTTTTTTACTTTTTGGACAAGGGAGAGGCTCTGACAGT

TTGGACTCAGATCGTCTATCCAGAAAACACTGGTCTGTATGTTATTGTGGAATCTTATGGCCCAAAAATA

TTACAAGAGAGTCATGAGATTTCCTTTGAAGCTGCCTTTGGATACTGCACCAAAACTCTGACACTAACAT

TTTATCAGAATGTAGATTATGAGAGAATATCTGATTACTTTGAGACACAAGACAAGCACACGGGTCTTGT

GCTGGTTCAGTTTCGACCTAGTGAATATTCAAAAGCATGTCCAATAGCCCAAAAGGTGTTCCAAATAGCT

GTTGGCTGTGATGATAAAAAATTCATTGCAATTAAAGGATTTAGTAAAAAAGGATGTCATCACCATGATT

TTTCATACGTGATTGAAAAGTCATATCTGAGGCATCAGCCATCGAAAAACTTGAGAGTAAGGTATATTTG

GGGAGAATATGGCTGCCCTCTGAGGCTTGACTTCACAGAAAAGTTTCAACCTGTGGTTCAACTATTTGAT

GATAATGGCTATGTTAAAGACGTTGAAGCAAATTTCATAGTGTGGGAAATACACGGCAGGGATGACTATA

GCTTTAATAATACTATGGCACAGAGTGGTTGTTTACATGAAGCACAGACATGGAAGTCAATGATTGAACT

TAACAAGCACCTCCCACTAGAAGAAGTCTGGGGACCTGAGAACTATAAACACTGTTTTTCTTATGCTATT

GGAAAACCAGGAGACTTAAATCAACCATACGAGATTATCAACAGTTCTAATGGTAACCATATATTTTGGC

CCATGGGCCATTCTGGAATGTATGTATTTCGTGTGAAGATCCTGGATCCAAACTATAGTTTCTGTAACCT

AACAGCTATGTTTGCAATAGAGACATTTGGACTGATTCCCAGTCCAAGTGTCTACCTGGTAGCTTCTTTC

CTCTTCGTCCTGATGCTGCTCTTCTTCACTATTCTTGTTTTGAGCTACTTTCGGTACATGAGGATTTATA

GACGATATATTTATGAACCACTTCACAAACCTCAAAGAAAACGTAAGAAGAATTAG

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.