SLC34A3 (sodium-dependent phosphate transport protein 2C) or NaPi2c is a 8-pass type II transmembrane protein belonging to the SLC34A transporter family. SLC34A3 acts as a type II sodium-phosphate cotransporter.  SLC34A3 is expressed primarily in the kidney and contributes to the maintenance of inorganic phosphate concentration in the kidney. SLC34A3 is involved in actively transporting phosphate into cells via Na+ cotransport in the renal brush border membrane.  SLC34A3 probably mediates 20-30% of the apical influx.Mutations in the SLC34A3 gene are associated with hereditary hypophosphatemic rickets with hypercalciuria (HHRH), an autosomal recessive form of hypophosphatemia characterized by reduced renal phosphate reabsorption and rickets.

SLC34A3 (sodium-dependent phosphate transport protein 2C) or NaPi2c is a 8-pass type II transmembrane protein belonging to the SLC34A transporter family. SLC34A3 acts as a type II sodium-phosphate cotransporter.  SLC34A3 is expressed primarily in the kidney and contributes to the maintenance of inorganic phosphate concentration in the kidney. SLC34A3 is involved in actively transporting phosphate into cells via Na+ cotransport in the renal brush border membrane.  SLC34A3 probably mediates 20-30% of the apical influx.Mutations in the SLC34A3 gene are associated with hereditary hypophosphatemic rickets with hypercalciuria (HHRH), an autosomal recessive form of hypophosphatemia characterized by reduced renal phosphate reabsorption and rickets.

 

Product Details

 

Gene Symbol: SLC34A3; NaPi2c; NPTIIc; HHRH; NPT-2c; NaPi-2c

 

NCBI Gene ID: 142680

 

Uniprot Entry: Q8N130

 

Construct Details: Full length human SLC34A3 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter +/- a selection marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1800 (bp)

 

Gene Insert Sequence:  

ATGCCGAGTTCCCTTCCCGGCAGCCAGGTCCCCCACCCCACTCTGGACGCGGTTGACCTAGTGGAAAAGA

CTCTGAGGAATGAAGGGACCTCCAGTTCTGCTCCAGTCTTGGAGGAAGGGGACACAGACCCCTGGACCCT

CCCTCAGCTGAAGGACACAAGCCAGCCCTGGAAAGAGCTCCGCGTGGCCGGCAGGCTGCGCCGCGTGGCC

GGCAGCGTCCTCAAGGCCTGCGGGCTCCTCGGCAGCCTGTACTTCTTCATCTGCTCTCTGGACGTCCTCA

GCTCCGCCTTCCAGCTGCTGGGCAGCAAAGTGGCCGGAGACATCTTCAAGGACAACGTGGTGCTGTCCAA

CCCTGTGGCTGGACTGGTCATTGGCGTGCTGGTCACAGCCCTGGTGCAGAGTTCCAGCACGTCCTCCTCC

ATCGTGGTCAGCATGGTGGCTGCTAAGCTGCTGACTGTCCGGGTGTCTGTGCCCATCATCATGGGTGTCA

ACGTAGGCACATCCATCACCAGCACCCTGGTCTCAATGGCGCAGTCAGGGGACCGGGATGAATTTCAGAG

GGCTTTCAGCGGCTCGGCGGTGCACGGGATCTTCAACTGGCTCACAGTGCTGGTCCTGCTGCCACTGGAG

AGCGCCACGGCCCTGCTGGAGAGGCTAAGTGAGCTAGCCCTGGGTGCCGCCAGCCTGACACCCAGGGCGC

AGGCGCCCGACATCCTCAAGGTGCTGACGAAGCCGCTCACACACCTCATCGTGCAGTTGGACTCCGACAT

GATCATGAGCAGTGCCACAGGCAACGCCACTAACAGCAGTCTCATTAAGCACTGGTGCGGCACCACGGGG

CAGCCGACCCAGGAGAACAGCAGCTGTGGCGCCTTCGGCCCGTGCACAGAGAAGAACAGCACAGCCCCGG

CGGACAGGCTGCCCTGCCGCCACCTGTTTGCGGGCACGGAGCTCACGGACCTGGCCGTGGGCTGCATCCT

GCTGGCCGGCTCCCTGCTGGTGCTCTGCGGCTGCCTGGTCCTCATAGTCAAGCTGCTCAACTCTGTGCTG

CGCGGCCGCGTGGCCCAGGTCGTGAGGACAGTCATCAATGCGGACTTCCCCTTCCCGCTGGGCTGGCTCG

GCGGCTACCTGGCCGTCCTCGCGGGCGCCGGCCTGACCTTCGCACTGCAGAGCAGCAGCGTCTTCACGGC

GGCCGTCGTGCCCCTCATGGGGGTCGGGGTGATCAGTCTGGACCGGGCGTACCCCCTCTTACTGGGCTCC

AACATCGGCACCACTACCACAGCCCTGCTGGCTGCCCTGGCCAGCCCCGCAGACAGGATGCTCAGCGCCC

TGCAGGTCGCCCTCATCCACTTCTTCTTCAACCTGGCCGGCATCCTGCTGTGGTACCTGGTGCCTGCACT

GCGGCTGCCCATCCCGCTGGCCAGGCACTTCGGGGTGGTGACCGCCCGTTACCGCTGGGTGGCTGGGGTC

TACCTGCTGCTCGGATTCCTGCTGCTGCCCCTGGCGGCCTTCGGGCTCTCCCTGGCAGGGGGCATGGAGC

TGGCCGCTGTCGGGGGTCCCCTGGTGGGGCTGGTGCTCCTCGTCATCCTGGTTACTGTCCTGCAGCGGCG

CCGGCCGGCCTGGCTGCCTGTCCGCCTGCGCTCCTGGGCCTGGCTCCCCGTCTGGCTCCATTCTCTGGAG

CCCTGGGACCGCCTGGTGACCCGCTGCTGCCCCTGCAACGTCTGCAGCCCCCCGAAGGCCACCACCAAAG

AGGCCTACTGCTACGAGAACCCTGAGATCTTGGCCTCCCAGCAGTTGTGA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.