CD300a, CLM8 (CMRF35-like molecule 8) or IgSF12 is a 299 amino acid (aa) single pass type I transmembrane glycoprotein that belongs to the CD300 family of the Immunoglobulin superfamily (IgSF).  The CD300 family members contain a single Ig-like V-type domain and are found on leukocytes involved in immune response signaling pathways. CD300a contains an N-terminal signal peptide, a 163-aa extracellular domain (ECD) with 1 Ig-like V-type domain and 2 potential glycosylation sites, a transmembrane domain (TM), and a 98-aa cytoplasmic tail. CD300a is expressed not only by natural killer (NK) cells but also by T-cell subsets, B-cells, dendritic cells, mast cells, granulocytes and monocytes. CD300a acta as an inhibitory receptor which may contribute to the down-regulation of cytolytic activity in NK cells, and to the down-regulation of mast cell degranulation. It also negatively regulates the Toll-like receptor (TLR) signaling mediated by MYD88.

CD300a, CLM8 (CMRF35-like molecule 8) or IgSF12 is a 299 amino acid (aa) single pass type I transmembrane glycoprotein that belongs to the CD300 family of the Immunoglobulin superfamily (IgSF).  The CD300 family members contain a single Ig-like V-type domain and are found on leukocytes involved in immune response signaling pathways. CD300a contains an N-terminal signal peptide, a 163-aa extracellular domain (ECD) with 1 Ig-like V-type domain and 2 potential glycosylation sites, a transmembrane domain (TM), and a 98-aa cytoplasmic tail. CD300a is expressed not only by natural killer (NK) cells but also by T-cell subsets, B-cells, dendritic cells, mast cells, granulocytes and monocytes. CD300a acta as an inhibitory receptor which may contribute to the down-regulation of cytolytic activity in NK cells, and to the down-regulation of mast cell degranulation. It also negatively regulates the Toll-like receptor (TLR) signaling mediated by MYD88.

 

Product Details

 

Gene Symbol: CD300a; IRC1; IRC2; CLM-8; CLM8; IRp60; IGSF12; CMRF35H; CMRF-35H; CMRF35-H; CMRF35H9; CMRF35-H9; IRC1/IRC2; CMRF-35-H9

 

NCBI Gene ID: 11314

 

Uniprot Entry: Q9UGN4

 

Construct Details: Full length human CD300a gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without an antibiotic selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter +/- a selection marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 900 (bp)

 

Gene Insert Sequence:  

ATGTGGCTGCCTTGGGCTCTGTTGCTTCTCTGGGTCCCAGGATGTTTTGCTCTGAGCAAATGCAGGACCG

TGGCGGGCCCCGTGGGGGGATCCCTGAGTGTGCAGTGTCCCTATGAGAAGGAACACAGGACCCTCAACAA

ATACTGGTGCAGACCACCACAGATTTTCCTATGTGACAAGATTGTGGAGACCAAAGGGTCAGCAGGAAAA

AGGAACGGCCGAGTGTCCATCAGGGACAGTCCTGCAAACCTCAGCTTCACAGTGACCCTGGAGAATCTCA

CAGAGGAGGATGCAGGCACCTACTGGTGTGGGGTGGATACACCATGGCTCCGAGACTTTCATGATCCCGT

TGTCGAGGTTGAGGTGTCCGTGTTCCCGGCATCAACGTCAATGACACCTGCAAGTATCACTGCGGCCAAG

ACCTCAACAATCACAACTGCATTTCCACCTGTATCATCCACTACCCTGTTTGCAGTGGGTGCCACCCACA

GTGCCAGCATCCAGGAGGAAACTGAGGAGGTGGTGAACTCACAGCTCCCGCTGCTCCTCTCCCTGCTGGC

ATTGTTGCTGCTTCTGTTGGTGGGGGCCTCCCTGCTAGCCTGGAGGATGTTTCAGAAATGGATCAAAGCT

GGTGACCATTCAGAGCTGTCCCAGAACCCCAAGCAGGCTGCCACGCAGAGTGAGCTGCACTACGCAAATC

TGGAGCTGCTGATGTGGCCTCTGCAGGAAAAGCCAGCACCACCAAGGGAGGTGGAGGTGGAATACAGCAC

TGTGGCCTCCCCCAGGGAAGAACTTCACTATGCCTCGGTGGTGTTTGATTCTAACACCAACAGGATAGCT

GCTCAGAGGCCTCGGGAGGAGGAACCAGATTCAGATTACAGTGTGATAAGGAAGACATAG

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.