GFP (green fluorescent protein) was discovered in Aequorea victoria (Jellyfish).  GFP transduces the blue chemiluminescence of aequorin into green fluorescent light upon receiving energy from the Ca2+-activated photoprotein aequorin. Its chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. GFP has been engineered to produce a large number of differently colored variants for making chimeric proteins as a fluorescent protein tag. GFP proteins can be expressed in most known cell types as a noninvasive marker in living cells and organisms. Therefore GFP proteins enable a wide range of applications where they can act as a cell lineage tracer, reporter of gene expression, as a measure of protein-protein interactions, or as a molecular biosensor and thermometer. 

GFP (green fluorescent protein) was discovered in Aequorea victoria (Jellyfish).  GFP transduces the blue chemiluminescence of aequorin into green fluorescent light upon receiving energy from the Ca2+-activated photoprotein aequorin. Its chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. GFP has been engineered to produce a large number of differently colored variants for making chimeric proteins as a fluorescent protein tag. GFP proteins can be expressed in most known cell types as a noninvasive marker in living cells and organisms. Therefore GFP proteins enable a wide range of applications where they can act as a cell lineage tracer, reporter of gene expression, as a measure of protein-protein interactions, or as a molecular biosensor and thermometer. 

Product Details

 

Gene Symbol: GFP

 

Uniprot Entry: P42212

 

Construct Details: GFP (encoding UniProt #P42212) is cloned into the Lentiviral expression vector pLTC with an EF1α promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells. It was co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of antibiotic stable-selection markers are available.

 

Vector Type: pLTE or pLTE-IRES-Marker (see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl inα sterile TE buffer (pH8.0)

 

Gene Insert Size: 720 (bp)

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR USE IN HUMAN. NOT RESELLABLE OR TRANSFERABLE

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

References

 

1. Gene 111:229 (1992)

2. Science 273:1392 (1996)

3. Nat. Biotechnol. 14:1246 (1996)

4. J. Am. Chem. Soc. 129:10302 (2007)

 

 

Documentation

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.