PROM2 (Prominin-2) is a member of the prominin family of pentaspan transmembrane glycoproteins. PROM2 is expressed in saliva within small membrane particles.  In submucosal tumor, PROM2 is expressed in spindle-shaped or stellate stromal cells. PROM2 is also expressed in prostate cancer cell lines.  PROM2 localizes to the apical and basolateral membranes of epithelial cells and may be involved in the organization of plasma membrane microdomains. PROM2 colocalizes with PROM1. PROM2 associates with membrane in a cholesterol-dependent manner. 

PROM2 (Prominin-2) is a member of the prominin family of pentaspan transmembrane glycoproteins. PROM2 is expressed in saliva within small membrane particles.  In submucosal tumor, PROM2 is expressed in spindle-shaped or stellate stromal cells. PROM2 is also expressed in prostate cancer cell lines.  PROM2 localizes to the apical and basolateral membranes of epithelial cells and may be involved in the organization of plasma membrane microdomains. PROM2 colocalizes with PROM1. PROM2 associates with membrane in a cholesterol-dependent manner. 

 

Product Details

 

Gene Symbol: PROM2; PROML2; hPROML2; prominin2

 

NCBI Gene ID: 150696

 

Uniprot Entry: Q8N271

 

Construct Details: Full length human PROM2 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 2505 (bp)

 

Gene Insert Sequence:  

ATGAAGCACACACTGGCTCTGCTGGCTCCCCTGCTGGGCCTGGGCCTGGGGCTGGCCCTGAGTCAGCTGG

CTGCAGGGGCCACAGACTGCAAGTTCCTTGGCCCGGCAGAGCACCTGACATTCACCCCAGCAGCCAGGGC

CCGGTGGCTGGCCCCTCGAGTTCGTGCGCCAGGACTCCTGGACTCCCTCTATGGCACCGTGCGCCGCTTC

CTCTCGGTGGTGCAGCTCAATCCTTTCCCTTCAGAGTTGGTAAAGGCCCTACTGAATGAGCTGGCCTCCG

TGAAGGTGAATGAGGTGGTGCGGTACGAGGCGGGCTACGTGGTATGCGCTGTGATCGCGGGCCTCTACCT

GCTGCTGGTGCCCACTGCCGGGCTTTGCTTCTGCTGCTGCCGCTGCCACCGGCGCTGCGGGGGACGAGTG

AAGACAGAGCACAAGGCGCTGGCCTGTGAGCGCGCGGCCCTCATGGTCTTCCTGCTGCTGACCACCCTCT

TGCTGCTGATTGGTGTGGTCTGTGCCTTTGTCACCAACCAGCGCACGCATGAACAGATGGGCCCCAGCAT

CGAGGCCATGCCTGAGACCCTGCTCAGCCTCTGGGGCCTGGTCTCTGATGTCCCCCAAGAGCTGCAGGCC

GTGGCACAGCAATTCTCCCTGCCCCAGGAGCAAGTCTCAGAGGAGCTGGATGGTGTTGGTGTGAGCATTG

GGAGCGCGATCCACACTCAGCTCAGGAGCTCCGTGTACCCCTTGCTGGCGGCCGTGGGCAGTTTGGGCCA

GGTCCTGCAGGTCTCCGTGCACCACCTGCAAACCTTGAATGCTACAGTGGTAGAGCTGCAGGCCGGGCAG

CAGGACCTGGAGCCAGCCATCCGGGAACACCGGGACCGCCTCCTTGAGCTGCTGCAGGAGGCCAGGTGCC

AGGGAGATTGTGCAGGGGCCCTGAGCTGGGCCCGCACCCTGGAGCTGGGTGCTGACTTCAGCCAGGTGCC

CTCTGTGGACCATGTCCTGCACCAGCTAAAAGGTGTCCCCGAGGCCAACTTCTCCAGCATGGTCCAGGAG

GAGAACAGCACCTTCAACGCCCTTCCAGCCCTGGCTGCCATGCAGACATCCAGCGTGGTGCAAGAGCTGA

AGAAGGCAGTGGCCCAGCAGCCGGAAGGGGTGAGGACACTGGCTGAAGGGTTCCCGGGCTTGGAGGCAGC

TTCCCGCTGGGCCCAGGCACTGCAGGAGGTGGAGGAGAGCAGCCGCCCCTACCTGCAGGAGGTGCAGAGA

TACGAGACCTACAGGTGGATCGTGGGCTGCGTGCTGTGCTCCGTGGTCCTATTCGTGGTGCTCTGCAACC

TGCTGGGCCTCAATCTGGGCATCTGGGGCCTGTCTGCCAGGGACGACCCCAGCCACCCAGAAGCCAAGGG

CGAGGCTGGAGCCCGCTTCCTCATGGCAGGTGTGGGCCTCAGCTTCCTCTTTGCTGCACCCCTCATCCTC

CTGGTGTTCGCCACCTTCCTGGTGGGTGGCAACGTGCAGACGCTGGTGTGCCAGAGCTGGGAGAACGGCG

AGCTCTTTGAGTTTGCAGACACCCCAGGGAACCTGCCCCCGTCCATGAACCTGTCGCAACTTCTTGGCCT

GAGGAAGAACATCAGCATCCACCAAGCCTATCAGCAGTGCAAGGAAGGGGCAGCGCTCTGGACAGTCCTG

CAGCTCAACGACTCCTACGACCTGGAGGAGCACCTGGATATCAACCAGTATACCAACAAGCTACGGCAGG

AGTTGCAGAGCCTGAAAGTAGACACACAGAGCCTGGACCTGCTGAGCTCAGCCGCCCGCCGGGACCTGGA

GGCCCTGCAGAGCAGTGGGCTTCAGCGCATCCACTACCCCGACTTCCTCGTTCAGATCCAGAGGCCCGTG

GTGAAGACCAGCATGGAGCAGCTGGCCCAGGAGCTGCAAGGACTGGCCCAGGCCCAAGACAATTCTGTGC

TGGGGCAGCGGCTGCAGGAGGAGGCCCAAGGACTCAGAAACCTTCACCAGGAGAAGGTCGTCCCCCAGCA

GAGCCTTGTGGCAAAGCTCAACCTCAGCGTCAGGGCCCTGGAGTCCTCTGCCCCGAATCTCCAGCTGGAG

ACCTCAGATGTCCTAGCCAATGTCACCTACCTGAAAGGAGAGCTGCCTGCCTGGGCAGCCAGGATCCTGA

GGAATGTGAGTGAGTGTTTCCTGGCCCGGGAGATGGGCTACTTCTCCCAGTACGTGGCCTGGGTGAGAGA

GGAGGTGACTCAGCGCATTGCCACCTGCCAGCCCCTCTCCGGAGCCCTGGACAACAGCCGTGTGATCCTG

TGTGACATGATGGCTGACCCCTGGAATGCCTTCTGGTTCTGCCTGGCATGGTGCACCTTCTTCCTGATCC

CCAGCATCATCTTTGCCGTCAAGACCTCCAAATACTTCCGTCCTATCCGGAAACGCCTCAGCTCCACCAG

CTCTGAGGAGACTCAGCTCTTCCACATCCCCCGGGTTACCTCCCTGAAGCTGTAG

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.