Cadherins are calcium-dependent cell adhesion proteins that preferentially interact with themselves in a homophilic manner in connecting cells.  Cadherins thus contribute to the sorting of heterogeneous cell types.  Cadherin-2 or CDH2 is a 906 amino acid (aa) single pass type I transmembrane protein of the cadherin superfamily.  CDH2 contains an N-terminal signal peptide, a 565-aa extracellular domain (ECD) with 5 cadherin domains and 7 potential glycosylation sites, a transmembrane domain (TM), and a 161-aa highly conserved cytoplasmic tail. CDH2 is identified in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN.  It also interacts with CDCP1 and PCDH8.  CDH2 plays a role in the establishment of left-right asymmetry, development of the nervous system and the formation of cartilage and bone.  CDH2 may be leaved by MMP24, leading to the generation of a soluble 90 kDa form of ECD and a 45 kDa membrane-bound carboxy-terminal fragment 1 (CTF1), which is further cleaved by gamma-secretase into a 35 kDa. Cleavage in neural stem cells by MMP24 affects CDH2-mediated anchorage of neural stem cells, leading to modulate neural stem cell quiescence.

Cadherins are calcium-dependent cell adhesion proteins that preferentially interact with themselves in a homophilic manner in connecting cells.  Cadherins thus contribute to the sorting of heterogeneous cell types.  Cadherin-2 or CDH2 is a 906 amino acid (aa) single pass type I transmembrane protein of the cadherin superfamily.  CDH2 contains an N-terminal signal peptide, a 565-aa extracellular domain (ECD) with 5 cadherin domains and 7 potential glycosylation sites, a transmembrane domain (TM), and a 161-aa highly conserved cytoplasmic tail. CDH2 is identified in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN.  It also interacts with CDCP1 and PCDH8.  CDH2 plays a role in the establishment of left-right asymmetry, development of the nervous system and the formation of cartilage and bone.  CDH2 may be leaved by MMP24, leading to the generation of a soluble 90 kDa form of ECD and a 45 kDa membrane-bound carboxy-terminal fragment 1 (CTF1), which is further cleaved by gamma-secretase into a 35 kDa. Cleavage in neural stem cells by MMP24 affects CDH2-mediated anchorage of neural stem cells, leading to modulate neural stem cell quiescence.

 

Product Details

 

Gene Symbol: CDH2; CD325; CDHN; NCAD; N-cadherin; CDw325; Cadherin-2

 

NCBI Gene ID: 1000

 

Uniprot Entry: P19022

 

Construct Details: Full length human CDH2/CD325 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter +/- a selection marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 2721 (bp)

 

Gene Insert Sequence:  

ATGTGCCGGATAGCGGGAGCGCTGCGGACCCTGCTGCCGCTGCTGGCGGCCCTGCTTCAGGCGTCTGTAG

AGGCTTCTGGTGAAATCGCATTATGCAAGACTGGATTTCCTGAAGATGTTTACAGTGCAGTCTTATCGAA

GGATGTGCATGAAGGACAGCCTCTTCTCAATGTGAAGTTTAGCAACTGCAATGGAAAAAGAAAAGTACAA

TATGAGAGCAGTGAGCCTGCAGATTTTAAGGTGGATGAAGATGGCATGGTGTATGCCGTGAGAAGCTTTC

CACTCTCTTCTGAGCATGCCAAGTTCCTGATATATGCCCAAGACAAAGAGACCCAGGAAAAGTGGCAAGT

GGCAGTAAAATTGAGCCTGAAGCCAACCTTAACTGAGGAGTCAGTGAAGGAGTCAGCAGAAGTTGAAGAA

ATAGTGTTCCCAAGACAATTCAGTAAGCACAGTGGCCACCTACAAAGGCAGAAGAGAGACTGGGTCATCC

CTCCAATCAACTTGCCAGAAAACTCCAGGGGACCTTTTCCTCAAGAGCTTGTCAGGATCAGGTCTGATAG

AGATAAAAACCTTTCACTGCGGTACAGTGTAACTGGGCCAGGAGCTGACCAGCCTCCAACTGGTATCTTC

ATTATCAACCCCATCTCGGGTCAGCTGTCGGTGACAAAGCCCCTGGATCGCGAGCAGATAGCCCGGTTTC

ATTTGAGGGCACATGCAGTAGATATTAATGGAAATCAAGTGGAGAACCCCATTGACATTGTCATCAATGT

TATTGACATGAATGACAACAGACCTGAGTTCTTACACCAGGTTTGGAATGGGACAGTTCCTGAGGGATCA

AAGCCTGGAACATATGTGATGACCGTAACAGCAATTGATGCTGACGATCCCAATGCCCTCAATGGGATGT

TGAGGTACAGAATCGTGTCTCAGGCTCCAAGCACCCCTTCACCCAACATGTTTACAATCAACAATGAGAC

TGGTGACATCATCACAGTGGCAGCTGGACTTGATCGAGAAAAAGTGCAACAGTATACGTTAATAATTCAA

GCTACAGACATGGAAGGCAATCCCACATATGGCCTTTCAAACACAGCCACGGCCGTCATCACAGTGACAG

ATGTCAATGACAATCCTCCAGAGTTTACTGCCATGACGTTTTATGGTGAAGTTCCTGAGAACAGGGTAGA

CATCATAGTAGCTAATCTAACTGTGACCGATAAGGATCAACCCCATACACCAGCCTGGAACGCAGTGTAC

AGAATCAGTGGCGGAGATCCTACTGGACGGTTCGCCATCCAGACCGACCCAAACAGCAACGACGGGTTAG

TCACCGTGGTCAAACCAATCGACTTTGAAACAAATAGGATGTTTGTCCTTACTGTTGCTGCAGAAAATCA

AGTGCCATTAGCCAAGGGAATTCAGCACCCCCCTCAGTCAACTGCAACCGTGTCTGTTACAGTTATTGAC

GTAAATGAAAACCCTTATTTTGCCCCCAATCCTAAGATCATTCGCCAAGAAGAAGGGCTTCATGCCGGTA

CCATGTTGACAACATTCACTGCTCAGGACCCAGATCGATATATGCAGCAAAATATTAGATACACTAAATT

ATCTGATCCTGCCAATTGGCTAAAAATAGATCCTGTGAATGGACAAATAACTACAATTGCTGTTTTGGAC

CGAGAATCACCAAATGTGAAAAACAATATATATAATGCTACTTTCCTTGCTTCTGACAATGGAATTCCTC

CTATGAGTGGAACAGGAACGCTGCAGATCTATTTACTTGATATTAATGACAATGCCCCTCAAGTGTTACC

TCAAGAGGCAGAGACTTGCGAAACTCCAGACCCCAATTCAATTAATATTACAGCACTTGATTATGACATT

GATCCAAATGCTGGACCATTTGCTTTTGATCTTCCTTTATCTCCAGTGACTATTAAGAGAAATTGGACCA

TCACTCGGCTTAATGGTGATTTTGCTCAGCTTAATTTAAAGATAAAATTTCTTGAAGCTGGTATCTATGA

AGTTCCCATCATAATCACAGATTCGGGTAATCCTCCCAAATCAAATATTTCCATCCTGCGTGTGAAGGTT

TGCCAGTGTGACTCCAACGGGGACTGCACAGATGTGGACAGGATTGTGGGTGCGGGGCTTGGCACCGGTG

CCATCATTGCCATCCTGCTCTGCATCATCATCCTGCTTATCCTTGTGCTGATGTTTGTGGTATGGATGAA

ACGCCGGGATAAAGAACGCCAGGCCAAACAACTTTTAATTGATCCAGAAGATGATGTAAGAGATAATATT

TTAAAATATGATGAAGAAGGTGGAGGAGAAGAAGACCAGGACTATGACTTGAGCCAGCTGCAGCAGCCTG

ACACTGTGGAGCCTGATGCCATCAAGCCTGTGGGAATCCGACGAATGGATGAAAGACCCATCCACGCCGA

GCCCCAGTATCCGGTCCGATCTGCAGCCCCACACCCTGGAGACATTGGGGACTTCATTAATGAGGGCCTT

AAAGCGGCTGACAATGACCCCACAGCTCCACCATATGACTCCCTGTTAGTGTTTGACTATGAAGGCAGTG

GCTCCACTGCTGGGTCCTTGAGCTCCCTTAATTCCTCAAGTAGTGGTGGTGAGCAGGACTATGATTACCT

GAACGACTGGGGGCCACGGTTCAAGAAACTTGCTGACATGTATGGTGGAGGTGATGACTGA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.