CDC1P1 (CUB domain-containing protein 1) or CD318 is an 836 amino acid (aa) single pass type I transmembrane protein. CDCP1 contains an N-terminal signal peptide, a 638-aa extracellular domain (ECD) with 3 CUB domains and 7 potential glycosylation sites, a transmembrane domain (TM), and a 148-aa cytoplasmic tail. DCP1 is highly expressed in mitotic cells with low expression during interphase. It is expressed in cells with phenotypes reminiscent of mesenchymal stem cells and neural stem cells. CDCP1 is up-regulated in a number of human tumor cell lines, as well as in colorectal cancer, breast carcinoma and lung cancer. CDCP1 interacts with CDH2/N-cadherin, CDH3/P-cadherin, SDC1/syndecan-1, SDC4/syndecan-4 and the serine protease ST14/MT-SP1.  CDCP1 acts as a substrate for Src family kinases such as SRC and YES as well as by the protein kinase C gamma/PRKCG.  CDCP1 may be involved in cell adhesion and cell matrix association. CDCP1 may play a role in the regulation of anchorage versus migration or proliferation versus differentiation via its phosphorylation.  CDCP1 belongs to the tetraspanin web involved in the tyrosine phosphorylation-dependent regulation of tumor invasion and metastasis. CDCP1 may be a novel marker for leukemia diagnosis and for immature hematopoietic stem cell subsets.  A soluble form of CDCP1 can be produced by proteolytic cleavage at an unidentified site on its N-terminal side and converted to p80 by plasmin, a trypsin-like protease.

CDC1P1 (CUB domain-containing protein 1) or CD318 is an 836 amino acid (aa) single pass type I transmembrane protein. CDCP1 contains an N-terminal signal peptide, a 638-aa extracellular domain (ECD) with 3 CUB domains and 7 potential glycosylation sites, a transmembrane domain (TM), and a 148-aa cytoplasmic tail. DCP1 is highly expressed in mitotic cells with low expression during interphase. It is expressed in cells with phenotypes reminiscent of mesenchymal stem cells and neural stem cells. CDCP1 is up-regulated in a number of human tumor cell lines, as well as in colorectal cancer, breast carcinoma and lung cancer. CDCP1 interacts with CDH2/N-cadherin, CDH3/P-cadherin, SDC1/syndecan-1, SDC4/syndecan-4 and the serine protease ST14/MT-SP1.  CDCP1 acts as a substrate for Src family kinases such as SRC and YES as well as by the protein kinase C gamma/PRKCG.  CDCP1 may be involved in cell adhesion and cell matrix association. CDCP1 may play a role in the regulation of anchorage versus migration or proliferation versus differentiation via its phosphorylation.  CDCP1 belongs to the tetraspanin web involved in the tyrosine phosphorylation-dependent regulation of tumor invasion and metastasis. CDCP1 may be a novel marker for leukemia diagnosis and for immature hematopoietic stem cell subsets.  A soluble form of CDCP1 can be produced by proteolytic cleavage at an unidentified site on its N-terminal side and converted to p80 by plasmin, a trypsin-like protease.

 

Product Details

 

Gene Symbol: CDCP1; CD318; TRASK; SIMA135; gp140

 

NCBI Gene ID: 64866

 

Uniprot Entry: Q9H5V8

 

Construct Details: Full length human CDCP1/CD3218 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter +/- a selection marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 2511 (bp)

 

Gene Insert Sequence:  

ATGGCCGGCCTGAACTGCGGGGTCTCTATCGCACTGCTAGGGGTTCTGCTGCTGGGTGCGGCGCGCCTGC

CGCGCGGGGCAGAAGCTTTTGAGATTGCTCTGCCACGAGAAAGCAACATTACAGTTCTCATAAAGCTGGG

GACCCCGACTCTGCTGGCAAAACCCTGTTACATCGTCATTTCTAAAAGACATATAACCATGTTGTCCATC

AAGTCTGGAGAAAGAATAGTCTTTACCTTTAGCTGCCAGAGTCCTGAGAATCACTTTGTCATAGAGATCC

AGAAAAATATTGACTGTATGTCAGGCCCATGTCCTTTTGGGGAGGTTCAGCTTCAGCCCTCGACATCGTT

GTTGCCTACCCTCAACAGAACTTTCATCTGGGATGTCAAAGCTCATAAGAGCATCGGTTTAGAGCTGCAG

TTTTCCATCCCTCGCCTGAGGCAGATCGGTCCGGGTGAGAGCTGCCCAGACGGAGTCACTCACTCCATCA

GCGGCCGAATCGATGCCACCGTGGTCAGGATCGGAACCTTCTGCAGCAATGGCACTGTGTCCCGGATCAA

GATGCAAGAAGGAGTGAAAATGGCCTTACACCTCCCATGGTTCCACCCCAGAAATGTCTCCGGCTTCAGC

ATTGCAAACCGCTCATCTATAAAACGTCTGTGCATCATCGAGTCTGTGTTTGAGGGTGAAGGCTCAGCAA

CCCTGATGTCTGCCAACTACCCAGAAGGCTTCCCTGAGGATGAGCTCATGACGTGGCAGTTTGTCGTTCC

TGCACACCTGCGGGCCAGCGTCTCCTTCCTCAACTTCAACCTCTCCAACTGTGAGAGGAAGGAGGAGCGG

GTTGAATACTACATCCCGGGCTCCACCACCAACCCCGAGGTGTTCAAGCTGGAGGACAAGCAGCCTGGGA

ACATGGCGGGGAACTTCAACCTCTCTCTGCAAGGCTGTGACCAAGATGCCCAAAGTCCAGGGATCCTCCG

GCTGCAGTTCCAAGTTTTGGTCCAACATCCACAAAATGAAAGCAATAAAATCTACGTGGTTGACTTGAGT

AATGAGCGAGCCATGTCACTCACCATCGAGCCACGGCCCGTCAAACAGAGCCGCAAGTTTGTCCCTGGCT

GTTTCGTGTGTCTAGAATCTCGGACCTGCAGTAGCAACCTCACCCTGACATCTGGCTCCAAACACAAAAT

CTCCTTCCTTTGTGATGATCTGACACGTCTGTGGATGAATGTGGAAAAAACCATAAGCTGCACAGACCAC

CGGTACTGCCAAAGGAAATCCTACTCACTCCAGGTGCCCAGTGACATCCTCCACCTGCCTGTGGAGCTGC

ATGACTTCTCCTGGAAGCTGCTGGTGCCCAAGGACAGGCTCAGCCTGGTGCTGGTGCCAGCCCAGAAGCT

GCAGCAGCATACACACGAGAAGCCCTGCAACACCAGCTTCAGCTACCTCGTGGCCAGTGCCATACCCAGC

CAGGACCTGTACTTCGGCTCCTTCTGCCCGGGAGGCTCTATCAAGCAGATCCAGGTGAAGCAGAACATCT

CGGTGACCCTTCGCACCTTTGCCCCCAGCTTCCAACAAGAGGCCTCCAGGCAGGGTCTGACGGTGTCCTT

TATACCTTATTTCAAAGAGGAAGGCGTTTTCACGGTGACCCCTGACACAAAAAGCAAGGTCTACCTGAGG

ACCCCCAACTGGGACCGGGGCCTGCCATCCCTCACCTCTGTGTCCTGGAACATCAGCGTGCCCAGAGACC

AGGTGGCCTGCCTGACTTTCTTTAAGGAGCGGAGCGGCGTGGTCTGCCAGACAGGGCGCGCATTCATGAT

CATCCAGGAGCAGCGGACCCGGGCTGAGGAGATCTTCAGCCTGGACGAGGATGTGCTCCCCAAGCCAAGC

TTCCACCATCACAGCTTCTGGGTCAACATCTCTAACTGCAGCCCCACGAGCGGCAAGCAGCTAGACCTGC

TCTTCTCGGTGACACTTACCCCAAGGACTGTGGACTTGACTGTCATCCTCATCGCAGCGGTGGGAGGTGG

AGTCTTACTGCTGTCTGCCCTCGGGCTCATCATTTGCTGTGTGAAAAAGAAGAAAAAGAAGACAAACAAG

GGCCCCGCTGTGGGTATCTACAATGACAACATCAATACTGAGATGCCGAGGCAGCCAAAAAAGTTTCAGA

AAGGGCGAAAGGACAATGACTCCCATGTGTATGCAGTCATCGAGGACACCATGGTATATGGGCATCTGCT

ACAGGATTCCAGCGGCTCCTTCCTGCAGCCAGAGGTGGACACCTACCGGCCGTTCCAGGGCACCATGGGG

GTCTGTCCTCCCTCCCCACCCACCATATGCTCCAGGGCCCCAACTGCAAAGTTGGCCACTGAGGAGCCAC

CTCCTCGCTCCCCTCCTGAGTCTGAGAGTGAACCGTACACCTTCTCCCATCCCAACAATGGGGATGTAAG

CAGCAAGGACACAGACATTCCCTTACTGAACACTCAGGAGCCCATGGAGCCAGCAGAATAA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.