LILRB2 (Leukocyte immunoglobulin like receptor B2), also known as CD85d, LIR2 (Leukocyte immunoglobulin-like receptor 2), ILT4 (Ig-like transcript 4), and MIR10 (myeloid inhibitory receptor 10), is a member of the leukocyte immunoglobulin (Ig)-like receptor (LIR) family of the Ig superfamily (IgSF).  It belongs to the subfamily B class of LIR receptors (LILRBs), which are single-pass type I trnasmembrane proteins with 2 or 4 extracellular Ig-like domains, a transmembrane domain (TM), and 2-4 cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). LILRB2 has 4 Ig-like C2 type domains and 3 TIIMs. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases to modulate cellular responses.  LILRB2 is expressed predominantly on B-cells and monocytes, and at lower levels on dendritic cells. It is also detected at low levelson natural killer (NK) cells.  Like LILRB1, LILRB2 binds to MHC class I molecules on antigen-presenting cells (APCs) and transduces a negative signal that inhibits stimulation of an immune response. LILRB2 may control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. LILRB2 recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. Engagement of LILRB2 present on natural killer cells or T-cells by class I MHC molecules protects the target cells from lysis. LILRB2 also binds FCGR1A.  

LILRB2 (Leukocyte immunoglobulin like receptor B2), also known as CD85d, LIR2 (Leukocyte immunoglobulin-like receptor 2), ILT4 (Ig-like transcript 4), and MIR10 (myeloid inhibitory receptor 10), is a member of the leukocyte immunoglobulin (Ig)-like receptor (LIR) family of the Ig superfamily (IgSF).  It belongs to the subfamily B class of LIR receptors (LILRBs), which are single-pass type I trnasmembrane proteins with 2 or 4 extracellular Ig-like domains, a transmembrane domain (TM), and 2-4 cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). LILRB2 has 4 Ig-like C2 type domains and 3 TIIMs. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases to modulate cellular responses.  LILRB2 is expressed predominantly on B-cells and monocytes, and at lower levels on dendritic cells. It is also detected at low levelson natural killer (NK) cells.  Like LILRB1, LILRB2 binds to MHC class I molecules on antigen-presenting cells (APCs) and transduces a negative signal that inhibits stimulation of an immune response. LILRB2 may control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. LILRB2 recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. Engagement of LILRB2 present on natural killer cells or T-cells by class I MHC molecules protects the target cells from lysis. LILRB2 also binds FCGR1A.  

 

Product Details

 

Gene Symbol: LILRB2; ILT4; LIR2; MIR10; CD85d; ILT-4; LIR-2; MIR-10

 

NCBI Gene ID: 

 

Uniprot Entry: Q8N423

 

Construct Details: The recombinant human LILRB2-Fc fusion is expressed as a 667 amino acid protein consisting of Gln22 - Val461 region of LILRB2 and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.

 

Source: Human cells stably expressing human LILRB2-Fc and growing in chemical-defined media with no animal components or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60 

QTGTIPKPTL WAEPDSVITQ GSPVTLSCQG SLEAQEYRLY REKKSASWIT RIRPELVKNG 

        70         80         90        100        110        120 

QFHIPSITWE HTGRYGCQYY SRARWSELSD PLVLVMTGAY PKPTLSAQPS PVVTSGGRVT 

       130        140        150        160        170        180 

LQCESQVAFG GFILCKEGED EHPQCLNSQP HARGSSRAIF SVGPVSPNRR WSHRCYGYDL 

       190        200        210        220        230        240 

NSPYVWSSPS DLLELLVPGV SKKPSLSVQP GPVMAPGESL TLQCVSDVGY DRFVLYKEGE 

       250        260        270        280        290        300 

RDLRQLPGRQ PQAGLSQANF TLGPVSRSYG GQYRCYGAHN LSSECSAPSD PLDILITGQI 

       310        320        330        340        350        360 

RGTPFISVQP GPTVASGENV TLLCQSWRQF HTFLLTKAGA ADAPLRLRSI HEYPKYQAEF 

       370        380        390        400        410        420 

PMSPVTSAHA GTYRCYGSLN SDPYLLSHPS EPLELVVSGP SMGSSPPPTG PISTPAGPED 

       430        440        450        460        470        480 

QPLTPTGSDP QSGLGRHLGV DKTHTCPPCP APELLGGPSV FLFPPKPKDT LMISRTPEVT 

       490        500        510        520        530        540 

CVVVDVSHED PEVKFNWYVD GVEVHNAKTK PREEQYNSTY RVVSVLTVLH QDWLNGKEYK 

       550        560        570        580        590        600 

CKVSNKALPA PIEKTISKAK GQPREPQVYT LPPSREEMTK NQVSLTCLVK GFYPSDIAVE 

       610        620        630        640        650        660 

WESNGQPENN YKTTPPVLDS DGSFFLYSKL TVDKSRWQQG NVFSCSVMHE ALHNHYTQKS 

 

LSLSPGK 

  

M.W.: Calculated molecular mass (kDa): 73.3; Estimated by SDS-PAGE under reducing condition (kDa): 2 major bands 75-85 & 50-60 kDa probably due to proteolytic cleavage within the ECD of LILRB2

 

Calculated PI: 7.31

 

Calculated extinction coefficients (M-1 cm-1, at 280nm): 103220

 

Purity: >90% judged by SDS-PAGE under reducing condition (see the gel image above)

 

Formulation: Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free). 

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

1. J. Exp. Med. 186:1809-1818(1997)

2. J. Immunol. 159:5192-5196(1997)

3. Eur. J. Immunol. 28:3423-3434(1998)

4. Immunity 11:603-613(1999) 

5. J. Immunol. 168:3351-3359(2002)

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.