FCAR (Fc alpha receptor) or CD89 is a single-pass type I transmembrane protein belonging to the immunoglobulin (Ig) superfamily (IgSF).  ICD89 contains two Ig-like C2-type domains in the extracellular region.  CD89 binds to the Fc region of immunoglobulins alpha (IgA) and mediates several functions including cytokine production. CD89 associates with ITAM­containing signaling subunit FcRγ (Ig epsilon receptor subunit gamma) to form a functional signaling complex.  CD89 is present on the surface of myeloid lineage cells such as neutrophils, monocytes, macrophages, and eosinophils, where it mediates immunologic responses to pathogens.  CD89 recognizes IgA-opsonized targets and triggers several immunologic defense processes, including phagocytosis, antibody-dependent cell-mediated cytotoxicity (ADCC), and stimulation of the release of inflammatory mediators. There are multiple alternatively splicing variants encoding different isoforms of CD89. 

FCAR (Fc alpha receptor) or CD89 is a single-pass type I transmembrane protein belonging to the immunoglobulin (Ig) superfamily (IgSF).  ICD89 contains two Ig-like C2-type domains in the extracellular region.  CD89 binds to the Fc region of immunoglobulins alpha (IgA) and mediates several functions including cytokine production. CD89 associates with ITAM­containing signaling subunit FcRγ (Ig epsilon receptor subunit gamma) to form a functional signaling complex.  CD89 is present on the surface of myeloid lineage cells such as neutrophils, monocytes, macrophages, and eosinophils, where it mediates immunologic responses to pathogens.  CD89 recognizes IgA-opsonized targets and triggers several immunologic defense processes, including phagocytosis, antibody-dependent cell-mediated cytotoxicity (ADCC), and stimulation of the release of inflammatory mediators. There are multiple alternatively splicing variants encoding different isoforms of CD89. 

 

Product Details

 

Gene Symbol: CD89; FCAR; FcalphaRI; CTB-61M7.2

 

NCBI Gene ID: 2204

 

Uniprot Entry: P24071

 

Construct Details: The recombinant human FCAR-Fc protein is expressed as a 433 amino acid protein consisting of Gln22 - Gln226 region of FCAR/CD89 and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.

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Source: Human cells stably expressing human FCAR/CD89-Fc and growing in chemical-defined media with no animal components or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60 

QEGDFPMPFI SAKSSPVIPL DGSVKIQCQA IREAYLTQLM IIKNSTYREI GRRLKFWNET  

        70         80         90        100        110        120 

DPEFVIDHMD ANKAGRYQCQ YRIGHYRFRY SDTLELVVTG LYGKPFLSAD RGLVLMPGEN 

       130        140        150        160        170        180 

ISLTCSSAHI PFDRFSLAKE GELSLPQHQS GEHPANFSLG PVDLNVSGIY RCYGWYNRSP 

       190        200        210        220        230        240 

YLWSFPSNAL ELVVTDSIHQ DYTTQSTGTH TCPPCPAPEL LGGPSVFLFP PKPKDTLMIS 

       250        260        270        280        290        300 

RTPEVTCVVV DVSHEDPEVK FNWYVDGVEV HNAKTKPREE QYNSTYRVVS VLTVLHQDWL 

       310        320        330        340        350        360 

NGKEYKCKVS NKALPAPIEK TISKAKGQPR EPQVYTLPPS REEMTKNQVS LTCLVKGFYP 

       370        380        390        400        410        420 

SDIAVEWESN GQPENNYKTT PPVLDSDGSF FLYSKLTVDK SRWQQGNVFS CSVMHEALHN 

       430 

HYTQKSLSLS PGK 

 

M.W.: Calculated molecular mass (kDa): 48.9; Estimated by SDS-PAGE under reducing condition (kDa): 60-65 

 

Calculated PI: 6.53

 

Calculated extinction coefficients (M-1 cm-1, at 280nm): 70415

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above)

 

Formulation: Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method 

 

Bioactivity: Binds human IgA and the Fc of IgA

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.