LY75 (Lymphocyte antigen 75), also known as CD205 and CLEC13B (C-type lectin domain family 13 member B), is a single-pass type I transmembrane protein of the C-type lectin superfamily. LY75/CD205 contains 10 C-type lectin domains, 1 fibronectin type-II domain and 1 ricin B-type lectin domain in the extracellular region.  LY75/CD205 is expressed in spleen, thymus, colon and peripheral blood lymphocytes. It is detected in myeloid and B-lymphoid cell lines. This LY75-CD302 gene locus represents naturally occurring read-through transcription between the neighboring LY75 and CD302 genes. Alternative splicing results in multiple transcript variants of LY75 encoding fusion products that share sequence identity with each individual gene product. The isoform 2 (produced by intergenic splicing of LY75 and CD302) and isoform 3 of LY75/CD205 are expressed in malignant Hodgkin lymphoma cells called Hodgkin and Reed-Sternberg (HRS) cells.  LY75/CD205 acts as an endocytic receptor to direct captured antigens from the extracellular space to a specialized antigen-processing compartment.  Its signaling activity causes reduced proliferation of B-lymphocytes.

LY75 (Lymphocyte antigen 75), also known as CD205 and CLEC13B (C-type lectin domain family 13 member B), is a single-pass type I transmembrane protein of the C-type lectin superfamily. LY75/CD205 contains 10 C-type lectin domains, 1 fibronectin type-II domain and 1 ricin B-type lectin domain in the extracellular region.  LY75/CD205 is expressed in spleen, thymus, colon and peripheral blood lymphocytes. It is detected in myeloid and B-lymphoid cell lines. This LY75-CD302 gene locus represents naturally occurring read-through transcription between the neighboring LY75 and CD302 genes. Alternative splicing results in multiple transcript variants of LY75 encoding fusion products that share sequence identity with each individual gene product. The isoform 2 (produced by intergenic splicing of LY75 and CD302) and isoform 3 of LY75/CD205 are expressed in malignant Hodgkin lymphoma cells called Hodgkin and Reed-Sternberg (HRS) cells.  LY75/CD205 acts as an endocytic receptor to direct captured antigens from the extracellular space to a specialized antigen-processing compartment.  Its signaling activity causes reduced proliferation of B-lymphocytes.

 

Product Details

 

Gene Symbol: LY75; CD205; DEC205; LY-75; CLEC13B; DEC-205; GP200-MR6

 

NCBI Gene ID: 4065

 

Uniprot Entry: O60449

 

Construct Details: Full length human CD205 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Gene Insert Size: 5169 (bp)

 

Gene Insert Sequence:  

ATGAGGACAGGCTGGGCGACCCCTCGCCGCCCGGCGGGGCTCCTCATGCTGCTCTTCTGGTTCTTCGATC

TCGCGGAGCCCTCTGGCCGCGCAGCTAATGACCCCTTCACCATCGTCCATGGAAATACGGGCAAGTGCAT

CAAGCCAGTGTATGGCTGGATAGTAGCAGACGACTGTGATGAAACTGAGGACAAGTTATGGAAGTGGGTG

TCCCAGCATCGGCTCTTTCATTTGCACTCCCAAAAGTGCCTTGGCCTCGATATTACCAAATCGGTAAATG

AGCTGAGAATGTTCAGCTGTGACTCCAGTGCCATGCTGTGGTGGAAATGTGAGCACCACTCTCTGTACGG

AGCTGCCCGGTACCGGCTGGCTCTGAAGGATGGACATGGCACAGCAATCTCAAATGCATCTGATGTCTGG

AAGAAAGGAGGCTCAGAGGAAAGCCTTTGTGACCAGCCTTATCATGAGATCTATACCAGAGATGGGAACT

CTTATGGGAGACCTTGTGAATTTCCATTCTTAATTGATGGGACCTGGCATCATGATTGCATTCTTGATGA

AGATCATAGTGGGCCATGGTGTGCCACCACCTTAAATTATGAATATGACCGAAAGTGGGGCATCTGCTTA

AAGCCTGAAAACGGTTGTGAAGATAATTGGGAAAAGAACGAGCAGTTTGGAAGTTGCTACCAATTTAATA

CTCAGACGGCTCTTTCTTGGAAAGAAGCTTATGTTTCATGTCAGAATCAAGGAGCTGATTTACTGAGCAT

CAACAGTGCTGCTGAATTAACTTACCTTAAAGAAAAAGAAGGCATTGCTAAGATTTTCTGGATTGGTTTA

AATCAGCTATACTCTGCTAGAGGCTGGGAATGGTCAGACCACAAACCATTAAACTTTCTCAACTGGGATC

CAGACAGGCCCAGTGCACCTACTATAGGTGGCTCCAGCTGTGCAAGAATGGATGCTGAGTCTGGTCTGTG

GCAGAGCTTTTCCTGTGAAGCTCAACTGCCCTATGTCTGCAGGAAACCATTAAATAATACAGTGGAGTTA

ACAGATGTCTGGACATACTCAGATACCCGCTGTGATGCAGGCTGGCTGCCAAATAATGGATTTTGCTATC

TGCTGGTAAATGAAAGTAATTCCTGGGATAAGGCACATGCGAAATGCAAAGCCTTCAGTAGTGACCTAAT

CAGCATTCATTCTCTAGCAGATGTGGAGGTGGTTGTCACAAAACTCCATAATGAGGATATCAAAGAAGAA

GTGTGGATAGGCCTTAAGAACATAAACATACCAACTTTATTTCAGTGGTCAGATGGTACTGAAGTTACTC

TAACATATTGGGATGAGAATGAGCCAAATGTTCCCTACAATAAGACGCCCAACTGTGTTTCCTACTTAGG

AGAGCTAGGTCAGTGGAAAGTCCAATCATGTGAGGAGAAACTAAAATATGTATGCAAGAGAAAGGGAGAA

AAACTGAATGACGCAAGTTCTGATAAGATGTGTCCTCCAGATGAGGGCTGGAAGAGACATGGAGAAACCT

GTTACAAGATTTATGAGGATGAGGTCCCTTTTGGAACAAACTGCAATCTGACTATCACTAGCAGATTTGA

GCAAGAATACCTAAATGATTTGATGAAAAAGTATGATAAATCTCTAAGAAAATACTTCTGGACTGGCCTG

AGAGATGTAGATTCTTGTGGAGAGTATAACTGGGCAACTGTTGGTGGAAGAAGGCGGGCTGTAACCTTTT

CCAACTGGAATTTTCTTGAGCCAGCTTCCCCGGGCGGCTGCGTGGCTATGTCTACTGGAAAGTCTGTTGG

AAAGTGGGAGGTGAAGGACTGCAGAAGCTTCAAAGCACTTTCAATTTGCAAGAAAATGAGTGGACCCCTT

GGGCCTGAAGAAGCATCCCCTAAGCCTGATGACCCCTGTCCTGAAGGCTGGCAGAGTTTCCCCGCAAGTC

TTTCTTGTTATAAGGTATTCCATGCAGAAAGAATTGTAAGAAAGAGGAACTGGGAAGAAGCTGAACGATT

CTGCCAAGCCCTTGGAGCACACCTTTCTAGCTTCAGCCATGTGGATGAAATAAAGGAATTTCTTCACTTT

TTAACGGACCAGTTCAGTGGCCAGCATTGGCTGTGGATTGGTTTGAATAAAAGGAGCCCAGATTTACAAG

GATCCTGGCAATGGAGTGATCGTACACCAGTGTCTACTATTATCATGCCAAATGAGTTTCAGCAGGATTA

TGACATCAGAGACTGTGCTGCTGTCAAGGTATTTCATAGGCCATGGCGAAGAGGCTGGCATTTCTATGAT

GATAGAGAATTTATTTATTTGAGGCCTTTTGCTTGTGATACAAAACTTGAATGGGTGTGCCAAATTCCAA

AAGGCCGTACTCCAAAAACACCAGACTGGTACAATCCAGACCGTGCTGGAATTCATGGACCTCCACTTAT

AATTGAAGGAAGTGAATATTGGTTTGTTGCTGATCTTCACCTAAACTATGAAGAAGCCGTCCTGTACTGT

GCCAGCAATCACAGCTTTCTTGCAACTATAACATCTTTTGTGGGACTAAAAGCCATCAAAAACAAAATAG

CAAATATATCTGGTGATGGACAGAAGTGGTGGATAAGAATTAGCGAGTGGCCAATAGATGATCATTTTAC

ATACTCACGATATCCATGGCACCGCTTTCCTGTGACATTTGGAGAGGAATGCTTGTACATGTCTGCCAAG

ACTTGGCTTATCGACTTAGGTAAACCAACAGACTGTAGTACCAAGTTGCCCTTCATCTGTGAAAAATATA

ATGTTTCTTCGTTAGAGAAATACAGCCCAGATTCTGCAGCTAAAGTGCAATGTTCTGAGCAATGGATTCC

TTTTCAGAATAAGTGTTTTCTAAAGATCAAACCCGTGTCTCTCACATTTTCTCAAGCAAGCGATACCTGT

CACTCCTATGGTGGCACCCTTCCTTCAGTGTTGAGCCAGATTGAACAAGACTTTATTACATCCTTGCTTC

CGGATATGGAAGCTACTTTATGGATTGGTTTGCGCTGGACTGCCTATGAAAAGATAAACAAATGGACAGA

TAACAGAGAGCTGACGTACAGTAACTTTCACCCATTATTGGTTAGTGGGAGGCTGAGAATACCAGAAAAT

TTTTTTGAGGAAGAGTCTCGCTACCACTGTGCCCTAATACTCAACCTCCAAAAATCACCGTTTACTGGGA

CGTGGAATTTTACATCCTGCAGTGAACGCCACTTTGTGTCTCTCTGTCAGAAATATTCAGAAGTTAAAAG

CAGACAGACGTTGCAGAATGCTTCAGAAACTGTAAAGTATCTAAATAATCTGTACAAAATAATCCCAAAG

ACTCTGACTTGGCACAGTGCTAAAAGGGAGTGTCTGAAAAGTAACATGCAGCTGGTGAGCATCACGGACC

CTTACCAGCAGGCATTCCTCAGTGTGCAGGCGCTCCTTCACAACTCTTCCTTATGGATCGGACTCTTCAG

TCAAGATGATGAACTCAACTTTGGTTGGTCAGATGGGAAACGTCTTCATTTTAGTCGCTGGGCTGAAACT

AATGGGCAACTCGAAGACTGTGTAGTATTAGACACTGATGGATTCTGGAAAACAGTTGATTGCAATGACA

ATCAACCAGGTGCTATTTGCTACTATTCAGGAAATGAGACTGAAAAAGAGGTCAAACCAGTTGACAGTGT

TAAATGTCCATCTCCTGTTCTAAATACTCCGTGGATACCATTTCAGAACTGTTGCTACAATTTCATAATA

ACAAAGAATAGGCATATGGCAACAACACAGGATGAAGTTCATACTAAATGCCAGAAACTGAATCCAAAAT

CACATATTCTGAGTATTCGAGATGAAAAGGAGAATAACTTTGTTCTTGAGCAACTGCTGTACTTCAATTA

TATGGCTTCATGGGTCATGTTAGGAATAACTTATAGAAATAAGTCTCTTATGTGGTTTGATAAGACCCCA

CTGTCATATACACATTGGAGAGCAGGAAGACCAACTATAAAAAATGAGAAGTTTTTGGCTGGTTTAAGTA

CTGACGGCTTCTGGGATATTCAAACCTTTAAAGTTATTGAAGAAGCAGTTTATTTTCACCAGCACAGCAT

TCTTGCTTGTAAAATTGAAATGGTTGACTACAAAGAAGAATATAATACTACACTGCCACAGTTTATGCCA

TATGAAGATGGTATTTACAGTGTTATTCAAAAAAAGGTAACATGGTATGAAGCATTAAACATGTGTTCTC

AAAGTGGAGGTCACTTGGCAAGCGTTCACAACCAAAATGGCCAGCTCTTTCTGGAAGATATTGTAAAACG

TGATGGATTTCCACTATGGGTTGGGCTCTCAAGTCATGATGGAAGTGAATCAAGTTTTGAATGGTCTGAT

GGTAGTACATTTGACTATATCCCATGGAAAGGCCAAACATCTCCTGGAAATTGTGTTCTCTTGGATCCAA

AAGGAACTTGGAAACATGAAAAATGCAACTCTGTTAAGGATGGTGCTATTTGTTATAAACCTACAAAATC

TAAAAAGCTGTCCCGTCTTACATATTCATCAAGATGTCCAGCAGCAAAAGAGAATGGGTCACGGTGGATC

CAGTACAAGGGTCACTGTTACAAGTCTGATCAGGCATTGCACAGTTTTTCAGAGGCCAAAAAATTGTGTT

CAAAACATGATCACTCTGCAACTATCGTTTCCATAAAAGATGAAGATGAGAATAAATTTGTGAGCAGACT

GATGAGGGAAAATAATAACATTACCATGAGAGTTTGGCTTGGATTATCTCAACATTCTGTTGACCAGTCT

TGGAGTTGGTTAGATGGATCAGAAGTGACATTTGTCAAATGGGAAAATAAAAGTAAGAGTGGTGTTGGAA

GATGTAGCATGTTGATAGCTTCAAATGAAACTTGGAAAAAAGTTGAATGTGAACATGGTTTTGGAAGAGT

TGTCTGCAAAGTGCCTCTGGGCCCTGATTACACAGCAATAGCTATCATAGTTGCCACACTAAGTATCTTA

GTTCTCATGGGCGGACTGATTTGGTTCCTCTTCCAAAGGCACCGTTTGCACCTGGCGGGTTTCTCATCAG

TTCGATATGCACAAGGAGTGAATGAAGATGAGATTATGCTTCCTTCTTTCCATGACTAA

 

Formulation: Lentivector encoded and pre-packaged viral particles (typical titer 10⁶ - 10⁷ IFU/ml) in the conditional medium (serum-free) from HEK293 cells that have been transfected with the lentivector gene clone and the LentiPAK DNA mix

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Important Safety Information: With the safety features in place, our lentiviral vectors and viral particles can be employed in standard Biosafety Level 2 tissue culture facilities and should be treated with the same level of caution as any other potentially infectious agent. Any investigator who purchases our lentiviral/retroviral products & services is responsible for following Biosafety Level 2 requirements on the handling of viral particles. For more information on Biosafety Level 2 agents and practices, please refer to NIH’s “Biosafety Considerations for Research with Lentiviral Vectors”.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Transduction Protocol

 

The product is shipped at 4°C for immediate use.  Upon receipt, centrifuge the vial briefly before opening.  Store at –80°C or lower and the product is stable for 3 months.  Avoid repeated freeze-thaw cycles.

 

 

The product should be employed in a Biosafety Level 2 tissue culture facility.

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.