MRC2 (mannose receptor C type 2), also known as CD280, UPARAP (Urokinase-type plasminogen activator receptor-associated protein) and CLEC13E (C-type lectin domain family 13 member E), is a single-pass type I transmembrane protein and a member of the mannose receptor family of the C-type lectin superfamily.  MRC2 contains  8 C-type lectin domains, 1 fibronectin type-II domain and 1 ricin B-type lectin domain in the extracellular region.  MRC2 plays a role in extracellular matrix remodeling by mediating the internalization and lysosomal degradation of collagen ligands. MRC2 m play a role as endocytotic lectin receptor displaying calcium-dependent lectin activity. It internalizes glycosylated ligands from the extracellular space for release in an endosomal compartment via clathrin-mediated endocytosis. MRC2 may be involved in plasminogen activation system controlling the extracellular level of PLAUR/PLAU, and thus may regulate protease activity at the cell surface. MRC2 may contribute to cellular uptake, remodeling and degradation of extracellular collagen matrices. It may play a role during cancer progression as well as in other chronic tissue destructive diseases acting on collagen turnover. MRC2 may participate in remodeling of extracellular matrix cooperating with the matrix metalloproteinases (MMPs).  Expression of MRC2 may play a role in the tumorigenesis and metastasis of several malignancies including breast cancer, gliomas and metastatic bone disease. 

MRC2 (mannose receptor C type 2), also known as CD280, UPARAP (Urokinase-type plasminogen activator receptor-associated protein) and CLEC13E (C-type lectin domain family 13 member E), is a single-pass type I transmembrane protein and a member of the mannose receptor family of the C-type lectin superfamily.  MRC2 contains  8 C-type lectin domains, 1 fibronectin type-II domain and 1 ricin B-type lectin domain in the extracellular region.  MRC2 plays a role in extracellular matrix remodeling by mediating the internalization and lysosomal degradation of collagen ligands. MRC2 m play a role as endocytotic lectin receptor displaying calcium-dependent lectin activity. It internalizes glycosylated ligands from the extracellular space for release in an endosomal compartment via clathrin-mediated endocytosis. MRC2 may be involved in plasminogen activation system controlling the extracellular level of PLAUR/PLAU, and thus may regulate protease activity at the cell surface. MRC2 may contribute to cellular uptake, remodeling and degradation of extracellular collagen matrices. It may play a role during cancer progression as well as in other chronic tissue destructive diseases acting on collagen turnover. MRC2 may participate in remodeling of extracellular matrix cooperating with the matrix metalloproteinases (MMPs).  Expression of MRC2 may play a role in the tumorigenesis and metastasis of several malignancies including breast cancer, gliomas and metastatic bone disease. 

 

Product Details

 

Gene Symbol: MRC2; CD280; UPARAP; CLEC13E; ENDO180

 

NCBI Gene ID: 9902

 

Uniprot Entry: Q9UBG0

 

Construct Details: Full length human CD280 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Gene Insert Size: 4440 (bp)

 

Gene Insert Sequence:  

ATGGGGCCCGGCCGGCCGGCCCCCGCGCCCTGGCCTCGTCACCTGCTGCGCTGCGTCCTGCTCCTCGGGT

GCCTGCACCTCGGCCGTCCCGGCGCCCCTGGGGACGCCGCCCTCCCGGAACCCAACGTCTTCCTCATCTT

CAGCCATGGACTGCAGGGCTGCCTGGAGGCCCAGGGCGGGCAGGTCAGAGTCACCCCGGCTTGCAATACC

AGCCTCCCTGCCCAGCGCTGGAAGTGGGTCTCCCGAAACCGGCTATTCAACCTGGGTACCATGCAGTGCC

TGGGCACAGGCTGGCCAGGCACCAACACCACGGCCTCCCTGGGCATGTATGAGTGTGACCGGGAAGCACT

GAATCTTCGCTGGCATTGTCGTACACTGGGTGACCAGCTGTCCTTGCTCCTGGGGGCCCGCACCAGCAAC

ATATCCAAGCCTGGCACCCTTGAGCGTGGTGACCAGACCCGCAGTGGCCAGTGGCGCATCTACGGCAGCG

AGGAGGACCTATGTGCTCTGCCCTACCACGAGGTCTACACCATCCAGGGAAACTCCCACGGAAAGCCGTG

CACCATCCCCTTCAAATATGACAACCAGTGGTTCCACGGCTGCACCAGCACGGGCCGCGAGGATGGTCAC

CTGTGGTGTGCCACCACCCAGGACTACGGCAAAGACGAGCGCTGGGGCTTCTGCCCCATCAAGAGTAACG

ACTGCGAGACCTTCTGGGACAAGGACCAGCTGACTGACAGCTGCTACCAGTTTAACTTCCAGTCCACGCT

GTCGTGGAGGGAGGCCTGGGCCAGCTGCGAGCAGCAGGGTGCGGATCTGCTGAGCATCACGGAGATCCAC

GAGCAGACCTACATCAACGGCCTCCTCACTGGGTACAGCTCCACCCTGTGGATCGGCTTGAATGACTTGG

ACACGAGCGGAGGCTGGCAGTGGTCGGACAACTCGCCCCTCAAGTACCTCAACTGGGAGAGTGACCAGCC

GGACAACCCCAGTGAGGAGAACTGTGGAGTGATCCGCACTGAGTCCTCGGGCGGCTGGCAGAACCGTGAC

TGCAGCATCGCGCTGCCCTATGTGTGCAAGAAGAAGCCCAACGCCACGGCCGAGCCCACCCCTCCAGACA

GGTGGGCCAATGTGAAGGTGGAGTGCGAGCCGAGCTGGCAGCCCTTCCAGGGCCACTGCTACCGCCTGCA

GGCCGAGAAGCGCAGCTGGCAGGAGTCCAAGAAGGCATGTCTACGGGGCGGTGGCGACCTGGTCAGCATC

CACAGCATGGCGGAGCTGGAATTCATCACCAAGCAGATCAAGCAAGAGGTGGAGGAGCTGTGGATCGGCC

TCAACGATTTGAAACTGCAGATGAATTTTGAGTGGTCTGACGGGAGCCTTGTGAGCTTCACCCACTGGCA

CCCCTTTGAGCCCAACAACTTCCGGGACAGTCTGGAGGACTGTGTCACCATCTGGGGCCCGGAAGGCCGC

TGGAACGACAGTCCCTGTAACCAGTCCTTGCCATCCATCTGCAAGAAGGCAGGCCAGCTGAGCCAGGGGG

CCGCCGAGGAGGACCATGGCTGCCGGAAGGGTTGGACGTGGCACAGCCCATCCTGCTACTGGCTGGGAGA

AGACCAAGTGACCTACAGTGAGGCCCGGCGCCTGTGCACTGACCATGGCTCTCAGCTGGTCACCATCACC

AACAGGTTCGAGCAGGCCTTCGTCAGCAGCCTCATCTACAACTGGGAGGGCGAGTACTTCTGGACGGCCC

TGCAGGACCTCAACAGCACCGGCTCCTTCTTCTGGCTCAGTGGGGATGAAGTCATGTACACCCACTGGAA

CCGGGACCAGCCCGGGTACAGCCGTGGGGGCTGCGTGGCGCTGGCCACTGGCAGCGCCATGGGGCTGTGG

GAGGTGAAGAACTGTACCTCGTTCCGGGCCCGCTACATCTGCCGGCAGAGCCTGGGCACTCCAGTGACGC

CGGAGCTGCCGGGGCCAGATCCCACGCCCAGCCTCACTGGCTCCTGTCCCCAGGGCTGGGCCTCGGACAC

CAAACTCCGGTATTGCTATAAGGTGTTCAGCTCAGAGCGGCTGCAGGACAAGAAGAGCTGGGTCCAGGCC

CAGGGGGCCTGCCAGGAGCTGGGGGCCCAGCTGCTGAGCCTGGCCAGCTACGAGGAGGAGCACTTTGTGG

CCAACATGCTCAACAAGATCTTCGGTGAATCAGAACCCGAGATCCACGAGCAGCACTGGTTCTGGATCGG

CCTGAACCGTCGGGATCCCAGAGGGGGTCAGAGTTGGCGCTGGAGCGACGGCGTAGGGTTCTCTTACCAC

AATTTCGACCGGAGCCGGCACGACGACGACGACATCCGAGGCTGTGCGGTGCTGGACCTGGCCTCCCTGC

AGTGGGTGGCCATGCAGTGCGACACACAGCTGGACTGGATCTGCAAGATCCCCAGAGGTACGGACGTGCG

GGAGCCCGACGACAGCCCTCAAGGCCGACGGGAATGGCTGCGCTTCCAGGAGGCCGAGTACAAGTTCTTT

GAGCACCACTCCACGTGGGCGCAGGCGCAGCGCATCTGCACGTGGTTCCAGGCCGAGCTGACCTCGGTGC

ACAGCCAGGCGGAGCTAGACTTCCTGAGCCACAACTTGCAGAAGTTCTCCCGGGCCCAGGAGCAGCACTG

GTGGATCGGCCTGCACACCTCTGAGAGCGATGGGCGCTTCAGATGGACAGATGGTTCCATTATAAACTTC

ATCTCCTGGGCACCAGGCAAACCTCGGCCTGTCGGCAAGGACAAGAAGTGCGTGTACATGACAGCCAGCC

GAGAGGACTGGGGGGACCAGAGGTGCCTGACAGCCTTGCCCTACATCTGCAAGCGCAGCAACGTCACCAA

AGAAACGCAGCCCCCAGACCTGCCAACTACAGCCCTGGGGGGCTGCCCCTCTGACTGGATCCAGTTCCTC

AACAAGTGTTTTCAGGTCCAGGGCCAGGAACCCCAGAGCCGGGTGAAGTGGTCAGAGGCACAGTTCTCCT

GTGAACAGCAAGAGGCCCAGCTGGTCACCATCACAAACCCCTTAGAGCAAGCATTCATCACAGCCAGCCT

GCCCAATGTGACCTTTGACCTTTGGATTGGCCTCCATGCCTCGCAGAGGGACTTCCAGTGGGTGGAGCAG

GAGCCTTTGATGTATGCCAACTGGGCACCTGGGGAGCCCTCTGGCCCTAGCCCTGCTCCCAGTGGCAACA

AACCGACCAGCTGTGCAGTGGTCCTGCACAGCCCCTCAGCCCACTTCACTGGCCGCTGGGACGATCGGAG

CTGCACGGAGGAGACCCATGGCTTCATCTGCCAGAAGGGCACGGACCCCTCCCTGAGCCCGTCCCCAGCA

GCGCTGCCCCCCGCCCCGGGCACTGAGCTCTCCTACCTCAACGGCACCTTCCGGCTGCTTCAGAAGCCGC

TGCGCTGGCACGATGCCCTCCTGCTGTGTGAGAGCCGCAATGCCAGCCTGGCCTACGTGCCCGACCCCTA

CACCCAGGCCTTCCTCACGCAGGCTGCCCGAGGGCTGCGCACGCCGCTCTGGATTGGGCTGGCTGGCGAG

GAGGGCTCTCGGCGGTACTCCTGGGTCTCAGAGGAGCCGCTGAACTACGTGGGCTGGCAGGACGGGGAGC

CGCAGCAGCCGGGGGGCTGTACCTACGTAGATGTGGACGGGGCCTGGCGCACCACCAGCTGTGACACCAA

GCTGCAGGGGGCTGTGTGTGGGGTTAGCAGTGGGCCCCCTCCTCCCCGAAGAATAAGCTACCATGGCAGC

TGTCCCCAGGGACTGGCAGACTCCGCGTGGATTCCCTTCCGGGAGCACTGCTATTCTTTCCACATGGAGC

TGCTGCTGGGCCACAAGGAGGCGCGACAGCGCTGCCAGAGAGCGGGTGGGGCCGTCCTGTCTATCCTGGA

TGAGATGGAGAATGTGTTTGTCTGGGAGCACCTGCAGAGCTATGAGGGCCAGAGTCGGGGCGCCTGGCTG

GGCATGAACTTCAACCCCAAAGGAGGCACTCTGGTCTGGCAGGACAACACAGCTGTGAACTACTCCAACT

GGGGGCCCCCGGGCTTGGGCCCCAGCATGCTGAGCCACAACAGCTGCTACTGGATTCAGAGCAACAGCGG

GCTATGGCGCCCCGGCGCTTGCACCAACATCACCATGGGTGTCGTCTGCAAGCTTCCTCGTGCTGAGCAG

AGCAGCTTCTCCCCATCAGCGCTTCCAGAGAACCCAGCGGCCCTGGTGGTGGTGCTGATGGCGGTGCTGC

TGCTCCTGGCCTTGCTGACCGCAGCCCTCATCCTTTACCGGAGGCGCCAGAGCATCGAGCGCGGGGCCTT

TGAGGGTGCCCGCTACAGCCGCAGCAGCTCCAGCCCCACCGAGGCCACTGAGAAGAACATCCTGGTGTCA

GACATGGAAATGAATGAGCAACAAGAATAG

 

Formulation: Lentivector encoded and pre-packaged viral particles (typical titer 10⁶ - 10⁷ IFU/ml) in the conditional medium (serum-free) from HEK293 cells that have been transfected with the lentivector gene clone and the LentiPAK DNA mix

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Important Safety Information: With the safety features in place, our lentiviral vectors and viral particles can be employed in standard Biosafety Level 2 tissue culture facilities and should be treated with the same level of caution as any other potentially infectious agent. Any investigator who purchases our lentiviral/retroviral products & services is responsible for following Biosafety Level 2 requirements on the handling of viral particles. For more information on Biosafety Level 2 agents and practices, please refer to NIH’s “Biosafety Considerations for Research with Lentiviral Vectors”.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Transduction Protocol

 

The product is shipped at 4°C for immediate use.  Upon receipt, centrifuge the vial briefly before opening.  Store at –80°C or lower and the product is stable for 3 months.  Avoid repeated freeze-thaw cycles.

 

 

The product should be employed in a Biosafety Level 2 tissue culture facility.

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.