B7­H6 (B7 homolog 6), also known as NCR3LG1 (Natural cytotoxicity triggering receptor 3 ligand 1), is a single pass, type I transmembrane glycoprotein that belongs to the B7 family of the Immunoglobulin (Ig) superfamily. Among the family members, they share about 20-25% amino acid (aa) identity. Like other B7 family members, B7-H6 contains one Ig V-like and one Ig C-like domain in the extracellular region. Within the ECD, human B7­H6 shares 99% aa identity with chimpanzee B7­-H6 and 53% -­ 56% with bovine, canine, and equine B7­-H6. Orthologs in rodents have not been identified. The Ig­ V-like domain of B7-H6 mediates the interaction with NKp30 (also known as NCR3 and CD337) expressed on NK cells, resulting in natural killer (NK) cell activation and cytotoxicity. B7-H6 does not show binding to other natural killer cell-activating receptors, such as NKp44, NKp46, or NKG2D. Ligation of NKp30 by B7­H6 induces NK cell activation and target cell cytolysis. B7-­H6 is expressed on a wide range of tumor cells, including T and B-lymphomas, myeloid leukemias, melanomas, carcinomas and large T SV40 antigen-transformed cells, The B7-H6 expression is consistent with the detection of NKp30 binding sites on these tumor cells and correlates with tumor cell sensitivity to NKp30-dependent cell lysis.  

B7­H6 (B7 homolog 6), also known as NCR3LG1 (Natural cytotoxicity triggering receptor 3 ligand 1), is a single pass, type I transmembrane glycoprotein that belongs to the B7 family of the Immunoglobulin (Ig) superfamily. Among the family members, they share about 20-25% amino acid (aa) identity. Like other B7 family members, B7-H6 contains one Ig V-like and one Ig C-like domain in the extracellular region. Within the ECD, human B7­H6 shares 99% aa identity with chimpanzee B7­-H6 and 53% -­ 56% with bovine, canine, and equine B7­-H6. Orthologs in rodents have not been identified. The Ig­ V-like domain of B7-H6 mediates the interaction with NKp30 (also known as NCR3 and CD337) expressed on NK cells, resulting in natural killer (NK) cell activation and cytotoxicity. B7-H6 does not show binding to other natural killer cell-activating receptors, such as NKp44, NKp46, or NKG2D. Ligation of NKp30 by B7­H6 induces NK cell activation and target cell cytolysis. B7-­H6 is expressed on a wide range of tumor cells, including T and B-lymphomas, myeloid leukemias, melanomas, carcinomas and large T SV40 antigen-transformed cells, The B7-H6 expression is consistent with the detection of NKp30 binding sites on these tumor cells and correlates with tumor cell sensitivity to NKp30-dependent cell lysis.  

 

Product Details

 

Gene Symbol: B7-H6; B7H6; NCR3LG1; DKFZp686O24166

 

NCBI Gene ID: 374383

 

Uniprot Entry: Q68D85

 

Construct Details: The recombinant human B7-H6-Fc fusion is expressed as a 466 amino acid protein consisting of Asp25 - Ser262 region of B7-H6 (UniProt accession #Q68D85) and a C-terminal Fc fusion from human IgG1, which exists as a dimer under non-reducing condition.

 

Source:  Human cells stably expressing B7-H6-Fc and growing in chemical-defined media with no animal components or antibiotics

 

Deduced Amino Acid Sequence:

DLKVEMMAGGTQITPLNDNVTIFCNIFYSQPLNITSMGITWFWKSLTFDKEVKVFEFFGDHQEAFRP

GAIVSPWRLKSGDASLRLPGIQLEEAGEYRCEVVVTPLKAQGTVQLEVVASPASRLLLDQVGMKENE

DKYMCESSGFYPEAINITWEKQTQKFPHPIEISEDVITGPTIKNMDGTFNVTSCLKLNSSQEDPGTV

YQCVVRHASLHTPLRSNFTLTAARHSLSETEKTDNFSSTGTHTCPPCPAPELLGGPSVFLFPPKPKD

TLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNG

KEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWES

NGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK

 

M.W.:  Calculated molecular mass 52.2 kDa; estimated by SDS-PAGE under reducing condition 75-85 kDa probably due to glycosylation

 

Calculated PI: 5.80

 

Calculated Extinction Coefficients (M-1 cm-1, at 280nm):  65485

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above)

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier free).

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Binds to its receptor NKp30/NCR3 and Induces IFN-γ secretion by NK-92 human natural killer lymphoma cells with an ED₅₀ of 0.4 - 2.6 µg/ml.

  

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks.

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. J. Exp. Med. 206:1495 (2009). 

2. J. Exp. Med. 208:703 (2011). 

3. Proc. Natl. Acad. Sci. 108:6223 (2011). 

4. Immunol. Rev. 241:180 (2011). 

5. J Exp Med. 11;208:703 (2011).

 

 

 FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

Documentation

 

Product datasheet (pdf) can be downloaded here: FCL1178-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.