MICA is the highly polymorphic major histocompatability complex class I chain-related protein A. MICA is a single pass type I transmembrane glycoprotein that belongs to the MIC subfamily of MHC-I family of Immunoglobulin superfamily (IgSF). MICA contains 1 Ig-like C1-type motifs (1 IgC1) in the extracellular domain (ECD) in addition to a transmembrane (TM) domain and a cytoplasmic tail. MICA is expressed on the cell surface, although unlike canonical class I molecules it does not seem to associate with beta-2-microglobulin. MICA acts as a ligand for the KLRK1/NKG2-D type II integral membrane protein receptor. Binding to KLRK1 leads to cell lysis.  MICA also functions as a stress-induced antigen that is broadly recognized by intestinal epithelial gamma delta T cells. Variations in this gene have been associated with susceptibility to psoriasis 1 and psoriatic arthritis, and the shedding of MICA-related antibodies and ligands is involved in the progression from monoclonal gammopathy of undetermined significance to multiple myeloma (MM).

MICA is the highly polymorphic major histocompatability complex class I chain-related protein A. MICA is a single pass type I transmembrane glycoprotein that belongs to the MIC subfamily of MHC-I family of Immunoglobulin superfamily (IgSF). MICA contains 1 Ig-like C1-type motifs (1 IgC1) in the extracellular domain (ECD) in addition to a transmembrane (TM) domain and a cytoplasmic tail. MICA is expressed on the cell surface, although unlike canonical class I molecules it does not seem to associate with beta-2-microglobulin. MICA acts as a ligand for the KLRK1/NKG2-D type II integral membrane protein receptor. Binding to KLRK1 leads to cell lysis.  MICA also functions as a stress-induced antigen that is broadly recognized by intestinal epithelial gamma delta T cells. Variations in this gene have been associated with susceptibility to psoriasis 1 and psoriatic arthritis, and the shedding of MICA-related antibodies and ligands is involved in the progression from monoclonal gammopathy of undetermined significance to multiple myeloma (MM).

 

Product Details

 

Gene Symbol: MICA; MIC-A; PERB11.1

 

NCBI Gene ID: 100507436

 

Uniprot Entry: Q29983

 

Construct Details: The recombinant human MICA-Fc fusion is expressed as a 511 amino acid protein consisting of Glu24 - His306 region of MICA and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.

 

Source: Human cells stably expressing human MICA-Fc and growing in chemical-defined media with no animal components or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60 

EPHSLRYNLT VLSWDGSVQS GFLTEVHLDG QPFLRCDRQK CRAKPQGQWA EDVLGNKTWD 

        70         80         90        100        110        120 

RETRDLTGNG KDLRMTLAHI KDQKEGLHSL QEIRVCEIHE DNSTRSSQHF YYDGELFLSQ 

       130        140        150        160        170        180 

NLETKEWTMP QSSRAQTLAM NVRNFLKEDA MKTKTHYHAM HADCLQELRR YLKSGVVLRR 

       190        200        210        220        230        240 

TVPPMVNVTR SEASEGNITV TCRASGFYPW NITLSWRQDG VSLSHDTQQW GDVLPDGNGT 

       250        260        270        280        290        300 

YQTWVATRIC QGEEQRFTCY MEHSGNHSTH PVPSGKVLVL QSHVDKTHTC PPCPAPELLG 

       310        320        330        340        350        360 

GPSVFLFPPK PKDTLMISRT PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN AKTKPREEQY 

       370        380        390        400        410        420 

NSTYRVVSVL TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI SKAKGQPREP QVYTLPPSRE 

       430        440        450        460        470        480 

EMTKNQVSLT CLVKGFYPSD IAVEWESNGQ PENNYKTTPP VLDSDGSFFL YSKLTVDKSR 

       490        500        510 

WQQGNVFSCS VMHEALHNHY TQKSLSLSPG K 

 

M.W.: Calculated molecular mass (kDa): 58.2; Estimated by SDS-PAGE under reducing condition (kDa): 85-90 (major) and 65-70 (minor)

 

Calculated PI: 6.92

 

Calculated extinction coefficients (M-1 cm-1, at 280nm): 92080

 

Purity: >90% judged by SDS-PAGE under reducing condition (see the gel image above)

 

Formulation: Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free). 

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method 

 

Bioactivity:  Binds to its receptor KLRK1/NKG2D protein.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.