CEA (carcinoembryonic antigen), also kown as CEACAM5, BGP1 and CD66e, is a member of the CEA gene family belonging to the immunoglobulin superfamily (IgSF). CEA family members are either transmembrane or GPI-anchored within two subgroups: the CEA cell adhesion molecules (CEACAM) and the pregnancy-specific glycoproteins.  In human, 7 CEACAMs have been identified including 3 transmembrane proteins (CEACAM1, 3, 4) and 4 GPI-anchored molecules (CEACAM5-8). Human CEA or CEACAM-5 contains 1Ig-like V-type (IgV) domain at the N-terminus, followed by 6 Ig-like C2-type (IgC2) domain and a GPI anchor.  It exists as a homodimer. Intriguingly orthologs of human CEA have not been found in other species. Human CEA is expressed primarily in epithelial cells and functions as an adhesion molecule through homophilic and heterophilic interactions with CEACAM1/CD66a. CEA plays an important role in the development and maintenance of epithelial architecture. CEA is up-regulated in a wide array of human tumors and has been used a s a common cancer biomarker. CEA can promote tumor cell migration, invasion, adhesion, and metastasis.  It may also contribute to tumorigenicity by inhibiting cellular differentiation. GPI-anchored CEA can be released by GPI­PLD, resulting in a soluble molecule that induces tumor metastasis.  CEA has been considered as a a target for immunotherapy. 

CEA (carcinoembryonic antigen), also kown as CEACAM5, BGP1 and CD66e, is a member of the CEA gene family belonging to the immunoglobulin superfamily (IgSF). CEA family members are either transmembrane or GPI-anchored within two subgroups: the CEA cell adhesion molecules (CEACAM) and the pregnancy-specific glycoproteins.  In human, 7 CEACAMs have been identified including 3 transmembrane proteins (CEACAM1, 3, 4) and 4 GPI-anchored molecules (CEACAM5-8). Human CEA or CEACAM-5 contains 1Ig-like V-type (IgV) domain at the N-terminus, followed by 6 Ig-like C2-type (IgC2) domain and a GPI anchor.  It exists as a homodimer. Intriguingly orthologs of human CEA have not been found in other species. Human CEA is expressed primarily in epithelial cells and functions as an adhesion molecule through homophilic and heterophilic interactions with CEACAM1/CD66a. CEA plays an important role in the development and maintenance of epithelial architecture. CEA is up-regulated in a wide array of human tumors and has been used a s a common cancer biomarker. CEA can promote tumor cell migration, invasion, adhesion, and metastasis.  It may also contribute to tumorigenicity by inhibiting cellular differentiation. GPI-anchored CEA can be released by GPI­PLD, resulting in a soluble molecule that induces tumor metastasis.  CEA has been considered as a a target for immunotherapy. 

 

Product Details

 

Gene Symbol: CEA; CEACAM5; CEACAM-5; CD66e; CEAM5

 

NCBI Gene ID: 1048

 

Uniprot Entry: P06731

 

Construct Details: Expressed as a single-chain Fv (scFv) from anti-CEA VH and VL region fused with a scFv of anti-CD3e VH and VL region with a His-tag at the C-terminus. Exists as a monomer under non-reducing and reducing condition.

  

Source:  Mammalian cells stably expressing anti-CEA:anti-CD3e  bispecific antibody (in a scFv-scFv’ format) and growing in chemical-defined media with no animal component or antibiotics

 

M.W.: Calculated molecular mass (kDa): 55.4; Estimated by SDS-PAGE under reducing condition(kDa): 55-60

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image inserted).

 

Formulation: Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier and preservative free). 

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Binds CEA extracellular domain (SKU#FCL2139) with high affinity and recognizes CD3e in the context of TCR complex on T cells.  Potently promotes T cell-mediated anti-tumor cytotoxicity after engaging with tumor cell surface antigen CEA

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks.

 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. Proc. Natl. Acad. Sci. 85:4648 (1988).

2. Mol. Cell. Biol. 10: 2738 (1990).

3. Cancer Res. 51:5679 (1991).

4. J. Exp. Med. 185:1557 (1997).

5. Semin. Cancer Biol. 9:67 (1999).

6. J. Biol. Chem. 275: 26935 (2000).

 

  

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.