The Polymerase Chain Reaction (PCR) provides a revolutionary technique for amplifying a single or a few copies of a template DNA across many orders of magnitude. We proudly present you with G&P High Fidelity (G&P HiFi™) PCR system, featuring the world’s most accurate DNA polymerase, to help you achieve success in all routine PCR applications. As a single-enzyme PCR system, G&P HiFi™ is developed to deliver industry-leading performance with unique features that no other enzyme can match:
» To learn more about above features and view data examples, please click here: G&P High Fidelity PCR System
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G&P HiFi™ is derived from a Thermococcus DNA polymerase and engineered for superior fidelity and performance. The enzyme is supplied with two proprietary buffers for optimal performance and compatibility with downstream applications (SKU#P01301 & #P01302). G&P HiFi™ is suited for high fidelity PCR of a wide range of DNA templates and amplicons (up to 15 kb using a single enzyme). The PCR products amplified by G&P HiFi™ are blunt-ended, suitable for DNA cloning, sequencing, mutagenesis and assembly.
We also offer G&P HiFi™ PCR kit and ready-to-use 2x master mix, which are formulated to accommodate convenient reaction setup and high throughput needs. G&P HiFi™ PCR kit (SKU#K01301) contains all reagents for high fidelity PCR and optimization by the user, if necessary. The 2x master mix (SKU#M01303) comes "pre-mixed", containing all ingredients for high fidelity PCR, including the enzyme, salt, Mg2+, dNTPs, stabilizers, and enhancers. To start a PCR, simply add your template DNA and primers.
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