GPR156 (probable G-protein coupled receptor 156) is a 7 transmembrane (7TM) receptor belonging to the family 3 (also known as family C) of the G-protein-coupled receptors (GPCRs). Structurally they are composed of 4 elements; an N-terminal signal peptide; a large hydrophilic extracellular agonist-binding region; a region containing 7TM domains; and a C-terminal cytoplasmic domain of variable length. Family 3 members include the metabotropic glutamate receptors (mGluRs), the extracellular calcium-sensing receptors (CASRs), the gamma-amino-butyric acid (GABA) type B receptors, and the vomeronasal type-2 receptors.  These GPCRs regulate many important physiological processes and many of them are promising targets for drug development. GPR156 is an orphan GPCR which specific function is unknown. 

GPR156 (probable G-protein coupled receptor 156) is a 7 transmembrane (7TM) receptor belonging to the family 3 (also known as family C) of the G-protein-coupled receptors (GPCRs). Structurally they are composed of 4 elements; an N-terminal signal peptide; a large hydrophilic extracellular agonist-binding region; a region containing 7TM domains; and a C-terminal cytoplasmic domain of variable length. Family 3 members include the metabotropic glutamate receptors (mGluRs), the extracellular calcium-sensing receptors (CASRs), the gamma-amino-butyric acid (GABA) type B receptors, and the vomeronasal type-2 receptors.  These GPCRs regulate many important physiological processes and many of them are promising targets for drug development. GPR156 is an orphan GPCR which specific function is unknown. 

 

Product Details

 

Gene Symbol: GPR156; PGR28; GABABL

 

NCBI Gene ID: 165829

 

Uniprot Entry: Q8NFN8

 

Construct Details: Full length human GPR156 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter +/- a selection marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 2445 (bp)

 

Gene Insert Sequence:  

ATGGAGCCTGAAATAAACTGCTCTGAATTGTGTGACAGTTTTCCTGGCCAGGAGCTGGATCGGAGACCCC

TTCATGATCTCTGCAAGACAACAATTACATCTTCCCACCACAGCAGTAAGACCATCTCTTCATTATCTCC

TGTCCTCTTGGGTATTGTTTGGACTTTTCTCAGCTGTGGACTTCTGCTGATACTTTTCTTTCTTGCCTTT

ACAATTCACTGCAGGAAGAACAGGATTGTGAAGATGTCCAGTCCCAATCTGAACATTGTGACCTTACTGG

GCAGTTGTCTCACTTACAGTAGCGCTTACCTCTTTGGGATTCAGGATGTTTTAGTGGGGAGCTCAATGGA

AACTCTCATTCAGACAAGACTGTCCATGCTGTGCATTGGGACCTCCCTTGTGTTTGGCCCCATTCTGGGA

AAGAGCTGGCGACTCTACAAGGTGTTTACCCAAAGGGTCCCGGACAAGAGAGTGATTATCAAAGACCTGC

AGTTGCTGGGGTTGGTGGCAGCCCTGTTGATGGCTGATGTGATCCTGCTCATGACGTGGGTGCTGACTGA

TCCCATCCAGTGCCTCCAGATTCTCAGTGTCAGTATGACGGTGACAGGGAAAGACGTGTCCTGCACTTCG

ACCAGCACCCACTTCTGTGCTTCCCGGTATTCCGATGTTTGGATTGCTCTCATTTGGGGATGCAAGGGTC

TGCTCCTGCTGTATGGTGCCTACCTGGCTGGCCTGACTGGCCATGTCAGCTCCCCTCCTGTGAATCAGTC

CTTAACCATCATGGTGGGGGTCAACCTCCTTGTACTGGCTGCTGGGCTGCTTTTTGTAGTCACCAGATAC

TTGCATTCCTGGCCCAACCTGGTCTTTGGACTCACATCTGGAGGGATCTTTGTTTGTACAACTACAATCA

ACTGCTTCATCTTCATTCCCCAGCTGAAGCAATGGAAGGCATTTGAAGAGGAAAACCAAACAATCAGACG

CATGGCCAAATATTTCAGCACTCCCAACAAAAGCTTCCATACCCAGTATGGTGAGGAGGAGAACTGCCAC

CCGAGGGGAGAGAAAAGCTCCATGGAGAGGCTCCTCACAGAAAAAAATGCTGTGATTGAAAGCCTGCAGG

AACAAGTAAACAACGCCAAAGAGAAGATTGTGAGGCTGATGTCAGCTGAGTGCACCTATGACCTCCCAGA

GGGGGCTGCCCCACCTGCCTCTTCCCCGAACAAGGACGTCCAGGCGGTAGCCTCGGTCCACACCCTGGCA

GCTGCTCAGGGGCCTTCGGGTCACCTCTCTGACTTTCAGAATGATCCTGGCATGGCTGCCCGGGATTCCC

AGTGCACTTCAGGGCCCTCCTCATATGCACAAAGCCTTGAGGGGCCTGGGAAGGACTCCAGCTTCTCCCC

AGGGAAGGAGGAGAAGATATCTGACTCAAAAGACTTTTCTGATCATTTAGACTCAGGTTGTAGCCAGAAG

CCATGGACTGAGCAAAGCCTGGGTCCAGAAAGAGGAGACCAAGTCCCCATGAACCCCAGCCAGAGTCTCC

TACCAGAAAGAGGCGGCTCAGATCCCCAGAGACAGAGGCATCTGGAGAACTCAGAGGAGCCCCCAGAGCG

GCGGTCACGGGTTAGTTCAGTAATCAGGGAGAAACTTCAGGAGGTCTTACAAGATCTGGGCCTGGGCCCT

GAGGCTTCCCTCTCCACCGCCCCCTCTTGTCATCAGCAAACCTGGAAGAACAGTGCTGCCTTCAGCCCCC

AAAAGATGCCCCTCTCCAAGGAGCTCGGCTTTAGCCCTTACATGGTGAGGAGAAGGCGGGCAGCTCAGCG

GGCCCGCTCACACTTTCCTGGCTCTGCACCCTCATCTGTGGGGCATCGGGCAAACAGGACTGTTCCTGGG

GCACACAGCAGGCTACATGTGCAGAATGGGGACAGCCCCAGCCTGGCCCCACAAACTACTGATTCCAGAG

TACGAAGACCTTCTTCCAGGAAGCCTTCACTACCTTCAGATCCTCAAGACAGACCAGGTACCCTGGAGGG

CAGCAAACAAAGCCAGACAGAGCCCGAGGGGGCTAGAGGGAGCAAAGCAGCCTTTCTTCGCCAGCCTTCT

GGTTCTGGCCGGGCCCCAAGTCCTGCTGCCCCATGCCTCTCCAAAGCCTCACCTGACTTGCCTGAACAGT

GGCAGCTGTGGCCCCCAGTGCCCTCAGGCTGTGCCTCCCTGTCTTCTCAACACAGCTATTTTGATACTGA

GTCCAGCAGCTCAGATGAGTTCTTCTGCCGCTGCCACCGGCCCTACTGTGAAATCTGCTTCCAGAGCTCT

TCTGACTCTAGTGACAGTGGCACATCAGACACTGACCCTGAGCCTACTGGGGGGCTGGCTTCCTGGGAAA

AGCTGTGGGCCCGCTCCAAGCCTATTGTGAACTTCAAAGATGACTTGAAACCCACGCTGGTGTGA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.