TPBG (trophoblast glycoprotein), also known as oncofetal antigen 5T4 and WAIF1 (wnt-activated inhibitory factor 1), is a single-pass type I transmembrane glycoprotein of the LRR (leucine-rich repeat) protein family.  TPBG is an oncofetal antigen that is specific to trophoblast cells except for amniotic epithelium. TPBG is expressed by all types of trophoblasts as early as 9 weeks of development.  In adults its expression is limited to a few epithelial cell types but highly abundant in many types of  tumor cells. TPBG’s expression is associated with poor clinical outcome in numerous cancers.  TPBG contains 1 LRRNT (LRR N-terminal cap), 7 LRR (LRR1-7), and 1 LRRCT (LRR C-terminal cap) domains in the extracellular region. TPBG may function as an inhibitor of Wnt/beta-catenin signaling by indirectly interacting with LRP6 and blocking Wnt3a-dependent LRP6 internalization.  The C-terminal part of LRRNT and LRR1 are essential for the inhibition of the Wnt signaling pathway.  TPBG may also be involved in cell adhesion and motility.

TPBG (trophoblast glycoprotein), also known as oncofetal antigen 5T4 and WAIF1 (wnt-activated inhibitory factor 1), is a single-pass type I transmembrane glycoprotein of the LRR (leucine-rich repeat) protein family.  TPBG is an oncofetal antigen that is specific to trophoblast cells except for amniotic epithelium. TPBG is expressed by all types of trophoblasts as early as 9 weeks of development.  In adults its expression is limited to a few epithelial cell types but highly abundant in many types of  tumor cells. TPBG’s expression is associated with poor clinical outcome in numerous cancers.  TPBG contains 1 LRRNT (LRR N-terminal cap), 7 LRR (LRR1-7), and 1 LRRCT (LRR C-terminal cap) domains in the extracellular region. TPBG may function as an inhibitor of Wnt/beta-catenin signaling by indirectly interacting with LRP6 and blocking Wnt3a-dependent LRP6 internalization.  The C-terminal part of LRRNT and LRR1 are essential for the inhibition of the Wnt signaling pathway.  TPBG may also be involved in cell adhesion and motility.

 

Product Details

 

Gene Symbol: TPBG; 5T4; M6P1; 5T4AG; WAIF1; 5T4M6P1

 

NCBI Gene ID: 7162

 

Uniprot Entry: Q13641

 

Construct Details: The recombinant human TPBG-Fc fusion is expressed as a 561 amino acid protein consisting of Ser32 - Ser355 region of TPBG and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing condition.  

 

Source: Human cells stably expressing human TPBG-Fc and growing in chemical-defined media with no animal components or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60 

SSPTSSASSF SSSAPFLASA VSAQPPLPDQ CPALCECSEA ARTVKCVNRN LTEVPTDLPA  

        70         80         90        100        110        120 

YVRNLFLTGN QLAVLPAGAF ARRPPLAELA ALNLSGSRLD EVRAGAFEHL PSLRQLDLSH 

       130        140        150        160        170        180 

NPLADLSPFA FSGSNASVSA PSPLVELILN HIVPPEDERQ NRSFEGMVVA ALLAGRALQG 

       190        200        210        220        230        240 

LRRLELASNH FLYLPRDVLA QLPSLRHLDL SNNSLVSLTY VSFRNLTHLE SLHLEDNALK 

       250        260        270        280        290        300 

VLHNGTLAEL QGLPHIRVFL DNNPWVCDCH MADMVTWLKE TEVVQGKDRL TCAYPEKMRN 

       310        320        330        340        350        360 

RVLLELNSAD LDCDPILPPS LQTSTTENLY FQGSTGTHTC PPCPAPELLG GPSVFLFPPK 

       370        380        390        400        410        420 

PKDTLMISRT PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN AKTKPREEQY NSTYRVVSVL 

       430        440        450        460        470        480 

TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI SKAKGQPREP QVYTLPPSRE EMTKNQVSLT 

       490        500        510        520        530        540 

CLVKGFYPSD IAVEWESNGQ PENNYKTTPP VLDSDGSFFL YSKLTVDKSR WQQGNVFSCS  

       550        560 

VMHEALHNHY TQKSLSLSPG K 

  

M.W.: Calculated molecular mass (kDa): 62; Estimated by SDS-PAGE under reducing condition (kDa): 90-100

 

Calculated PI: 5.96

 

Calculated extinction coefficients (M-1 cm-1, at 280nm): 54735

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above)

 

Formulation: Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free). 

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. J. Biol. Chem. 269:9319-9324(1994)

2. Mol. Cancer Ther. 1:1129-1137(2002)

3. Dev. Cell 21:1129-1143(2011)

4. Structure 22:612-620(2014) 

5. Semin Cancer Biol. 29:13-20 (2014).

 

 

Additional supporting documents, including product data sheet (PDS), COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.