Connective Tissue Growth Factor (CTGF) is a member of the CCN (CYR61/CTGF/NOV) family of secreted proteins that share a multimodular organization. Each CCN member contains an IGF­binding protein domain, a von Willebrand factor type C domain, a thrombospondin type I domain, and a cysteine knot domain. The multimodular CTGF binds multiple ligands and shows effects in development, differentiation, and tumorigenesis. CTGF is a mitogen that is secreted by vascular endothelial cells. It plays a role in chondrocyte proliferation and differentiation, cell adhesion in many cell types, and is related to platelet-derived growth factor. The C­terminal cysteine knot motif contains the heparin and low-density lipoprotein receptor (LDLR) binding sites that contributes to the adhesive and mitogenic properties of CTGF.  Certain polymorphisms in this gene have been linked with a higher incidence of systemic sclerosis. CTGF is the central mediator of tissue remodeling and fibrosis.  It is implicated in tumor progression and metastasis.  It promotes epithelial to mesenchymal transition (EMT), a process whereby normal epithelial cells become migratory, matrix-producing fibroblasts important for invasion and metastasis.  Elevated CTGF levels are found in desmoplastic cancer where it supports the formation of the extensive fibrous connective tissue (desmoplasia) around cancer.  Blockade of the expression or activity of CTGF reduces tumor growth and metastasis in serveral cancer animal model.

Connective Tissue Growth Factor (CTGF) is a member of the CCN (CYR61/CTGF/NOV) family of secreted proteins that share a multimodular organization. Each CCN member contains an IGF­binding protein domain, a von Willebrand factor type C domain, a thrombospondin type I domain, and a cysteine knot domain. The multimodular CTGF binds multiple ligands and shows effects in development, differentiation, and tumorigenesis. CTGF is a mitogen that is secreted by vascular endothelial cells. It plays a role in chondrocyte proliferation and differentiation, cell adhesion in many cell types, and is related to platelet-derived growth factor. The C­terminal cysteine knot motif contains the heparin and low-density lipoprotein receptor (LDLR) binding sites that contributes to the adhesive and mitogenic properties of CTGF.  Certain polymorphisms in this gene have been linked with a higher incidence of systemic sclerosis. CTGF is the central mediator of tissue remodeling and fibrosis.  It is implicated in tumor progression and metastasis.  It promotes epithelial to mesenchymal transition (EMT), a process whereby normal epithelial cells become migratory, matrix-producing fibroblasts important for invasion and metastasis.  Elevated CTGF levels are found in desmoplastic cancer where it supports the formation of the extensive fibrous connective tissue (desmoplasia) around cancer.  Blockade of the expression or activity of CTGF reduces tumor growth and metastasis in serveral cancer animal model.

 

Product Details

 

Gene Symbol: CCN2; NOV2; HCS24; IGFBP; IBP-8; IGFBP-8

 

NCBI Gene ID: 1490

 

Uniprot Entry: Q5M8T4

 

Construct Details: The recombinant human CTGF-Fc fusion is expressed as a 561 amino acid protein consisting of Gln27 - Ala349 region of CTGF2 and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions  

 

Source: Human cells stably expressing human CTGF-Fc and growing in chemical-defined media with no animal components or antibiotics

 

Deduced Amino Acid Sequence: 

 

        10         20         30         40         50         60 

QNCSGPCRCP DEPAPRCPAG VSLVLDGCGC CRVCAKQLGE LCTERDPCDP HKGLFCDFGS 

        70         80         90        100        110        120 

PANRKIGVCT AKDGAPCIFG GTVYRSGESF QSSCKYQCTC LDGAVGCMPL CSMDVRLPSP 

       130        140        150        160        170        180 

DCPFPRRVKL PGKCCEEWVC DEPKDQTVVG PALAAYRLED TFGPDPTMIR ANCLVQTTEW 

       190        200        210        220        230        240 

SACSKTCGMG ISTRVTNDNA SCRLEKQSRL CMVRPCEADL EENIKKGKKC IRTPKISKPI 

       250        260        270        280        290        300 

KFELSGCTSM KTYRAKFCGV CTDGRCCTPH RTTTLPVEFK CPDGEVMKKN MMFIKTCACH  

       310        320        330        340        350        360 

YNCPGDNDIF ESLYYRKMYG DMASTTENLY FQGSTGTHTC PPCPAPELLG GPSVFLFPPK 

       370        380        390        400        410        420 

PKDTLMISRT PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN AKTKPREEQY NSTYRVVSVL 

       430        440        450        460        470        480 

TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI SKAKGQPREP QVYTLPPSRE EMTKNQVSLT 

       490        500        510        520        530        540 

CLVKGFYPSD IAVEWESNGQ PENNYKTTPP VLDSDGSFFL YSKLTVDKSR WQQGNVFSCS 

       550        560 

VMHEALHNHY TQKSLSLSPG K 

  

M.W.: Calculated molecular mass (kDa): 62.2 Estimated by SDS-PAGE under reducing condition (kDa): 65, 50 kDa (two distinct bands)

 

Calculated PI: 8.07

 

Calculated extinction coefficients (M-1 cm-1, at 280nm): 62570

 

Purity: >90% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "DTT: +")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free). 

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Binds to anti-CTGF antibodies (SKU#MAB1466 & MAB1998) in a functional ELISA

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. J. Cell Biol. 114:1285 (1991).

2. J Cell Physiol. 181:153 (1999).

3. Endocr. Rev. 20:189 (1999).

4. Cancer Res. 66: 5817 (2006)

5. J. Hum. Genet. 51: 383 (2006) 

6. Dev. Cell 14: 818 (2008) 

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.