PTCH/PTCH1/PTC/PTC1 is a member of the patched (PTCH) gene family. The PTCH gene encodes a 12-pass tranmembrrane protein containing 1 SSD (sterol-sensing) domain.  PTCH acts as a receptor for sonic hedgehog (SHH), a secreted molecule implicated in the formation of embryonic structures and in tumorigenesis, as well as indian hedgehog (IHH) and desert hedgehog (DHH). PTCH also associates with the smoothened (SMO) protien to transduce the hedgehog's proteins signal. PTCH may function as a tumor suppressor as inactivation of this gene is probably a necessary, if not sufficient step for tumorigenesis. Mutations of the PTCH gene are associated with basal cell nevus syndrome (BCNS), esophageal squamous cell carcinoma, trichoepitheliomas, transitional cell carcinomas of the bladder, as well as holoprosencephaly 7 (HPE7). Multiple transcript variants due to alternative splicing have been described but the full-length sequences and biological activity remain undetermined.

PTCH/PTCH1/PTC/PTC1 is a member of the patched (PTCH) gene family. The PTCH gene encodes a 12-pass tranmembrrane protein containing 1 SSD (sterol-sensing) domain.  PTCH acts as a receptor for sonic hedgehog (SHH), a secreted molecule implicated in the formation of embryonic structures and in tumorigenesis, as well as indian hedgehog (IHH) and desert hedgehog (DHH). PTCH also associates with the smoothened (SMO) protien to transduce the hedgehog's proteins signal. PTCH may function as a tumor suppressor as inactivation of this gene is probably a necessary, if not sufficient step for tumorigenesis. Mutations of the PTCH gene are associated with basal cell nevus syndrome (BCNS), esophageal squamous cell carcinoma, trichoepitheliomas, transitional cell carcinomas of the bladder, as well as holoprosencephaly 7 (HPE7). Multiple transcript variants due to alternative splicing have been described but the full-length sequences and biological activity remain undetermined.

 

Product Details

 

Gene Symbol: PTCH; PTCH1; PTC; BCNS; HPE7; PTC1; NBCCS; PTCH11

 

NCBI Gene ID: 19206

 

Uniprot Entry: Q61115

 

Construct Details: Full length mouse PTCH gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 4305 (bp)

 

Gene Insert Sequence:  

ATGGCCTCGGCTGGTAACGCCGCCGGGGCCCTGGGCAGGCAGGCCGGCGGCGGGAGGCGCAGACGGACCG

GGGGACCGCACCGCGCCGCGCCGGACCGGGACTATCTGCACCGGCCCAGCTACTGCGACGCCGCCTTCGC

TCTGGAGCAGATTTCCAAGGGGAAGGCTACTGGCCGGAAAGCGCCGCTGTGGCTGAGAGCGAAGTTTCAG

AGACTCTTATTTAAACTGGGTTGTTACATTCAAAAGAACTGCGGCAAGTTTTTGGTTGTGGGTCTCCTCA

TATTTGGGGCCTTCGCTGTGGGATTAAAGGCAGCTAATCTCGAGACCAACGTGGAGGAGCTGTGGGTGGA

AGTTGGTGGACGAGTGAGTCGAGAATTAAATTATACCCGTCAGAAGATAGGAGAAGAGGCTATGTTTAAT

CCTCAACTCATGATACAGACTCCAAAAGAAGAAGGCGCTAATGTTCTGACCACAGAGGCTCTCCTGCAAC

ACCTGGACTCAGCACTCCAGGCCAGTCGTGTGCACGTCTACATGTATAACAGGCAATGGAAGTTGGAACA

TTTGTGCTACAAATCAGGGGAACTTATCACGGAGACAGGTTACATGGATCAGATAATAGAATACCTTTAC

CCTTGCTTAATCATTACACCTTTGGACTGCTTCTGGGAAGGGGCAAAGCTACAGTCCGGGACAGCATACC

TCCTAGGTAAGCCTCCTTTACGGTGGACAAACTTTGACCCCTTGGAATTCCTAGAAGAGTTAAAGAAAAT

AAACTACCAAGTGGACAGCTGGGAGGAAATGCTGAATAAAGCCGAAGTTGGCCATGGGTACATGGACCGG

CCTTGCCTCAACCCAGCCGACCCAGATTGCCCTGCCACAGCCCCTAACAAAAATTCAACCAAACCTCTTG

ATGTGGCCCTTGTTTTGAATGGTGGATGTCAAGGTTTATCCAGGAAGTATATGCATTGGCAGGAGGAGTT

GATTGTGGGTGGTACCGTCAAGAATGCCACTGGAAAACTTGTCAGCGCTCACGCCCTGCAAACCATGTTC

CAGTTAATGACTCCCAAGCAAATGTATGAACACTTCAGGGGCTACGACTATGTCTCTCACATCAACTGGA

ATGAAGACAGGGCAGCCGCCATCCTGGAGGCCTGGCAGAGGACTTACGTGGAGGTGGTTCATCAAAGTGT

CGCCCCAAACTCCACTCAAAAGGTGCTTCCCTTCACAACCACGACCCTGGACGACATCCTAAAATCCTTC

TCTGATGTCAGTGTCATCCGAGTGGCCAGCGGCTACCTACTGATGCTTGCCTATGCCTGTTTAACCATGC

TGCGCTGGGACTGCTCCAAGTCCCAGGGTGCCGTGGGGCTGGCTGGCGTCCTGTTGGTTGCGCTGTCAGT

GGCTGCAGGATTGGGCCTCTGCTCCTTGATTGGCATTTCTTTTAATGCTGCGACAACTCAGGTTTTGCCG

TTTCTTGCTCTTGGTGTTGGTGTGGATGATGTCTTCCTCCTGGCCCATGCATTCAGTGAAACAGGACAGA

ATAAGAGGATTCCATTTGAGGACAGGACTGGGGAGTGCCTCAAGCGCACCGGAGCCAGCGTGGCCCTCAC

CTCCATCAGCAATGTCACCGCCTTCTTCATGGCCGCATTGATCCCTATCCCTGCCCTGCGAGCGTTCTCC

CTCCAGGCTGCTGTGGTGGTGGTATTCAATTTTGCTATGGTTCTGCTCATTTTTCCTGCAATTCTCAGCA

TGGATTTATACAGACGTGAGGACAGAAGATTGGATATTTTCTGCTGTTTCACAAGCCCCTGTGTCAGCAG

GGTGATTCAAGTTGAGCCACAGGCCTACACAGAGCCTCACAGTAACACCCGGTACAGCCCCCCACCCCCA

TACACCAGCCACAGCTTCGCCCACGAAACCCATATCACTATGCAGTCCACCGTTCAGCTCCGCACAGAGT

ATGACCCTCACACGCACGTGTACTACACCACCGCCGAGCCACGCTCTGAGATCTCTGTACAGCCTGTTAC

CGTCACCCAGGACAACCTCAGCTGTCAGAGTCCCGAGAGCACCAGCTCTACCAGGGACCTGCTCTCCCAG

TTCTCAGACTCCAGCCTCCACTGCCTCGAGCCCCCCTGCACCAAGTGGACACTCTCTTCGTTTGCAGAGA

AGCACTATGCTCCTTTCCTCCTGAAACCCAAAGCCAAGGTTGTGGTAATCCTTCTTTTCCTGGGCTTGCT

GGGGGTCAGCCTTTATGGGACCACCCGAGTGAGAGACGGGCTGGACCTCACGGACATTGTTCCCCGGGAA

ACCAGAGAATATGACTTCATAGCTGCCCAGTTCAAGTACTTCTCTTTCTACAACATGTATATAGTCACCC

AGAAAGCAGACTACCCGAATATCCAGCACCTACTTTACGACCTTCATAAGAGTTTCAGCAATGTGAAGTA

TGTCATGCTGGAGGAGAACAAGCAACTTCCCCAAATGTGGCTGCACTACTTTAGAGACTGGCTTCAAGGA

CTTCAGGATGCATTTGACAGTGACTGGGAAACTGGGAGGATCATGCCAAACAATTATAAAAATGGATCAG

ATGACGGGGTCCTCGCTTACAAACTCCTGGTGCAGACTGGCAGCCGAGACAAGCCCATCGACATTAGTCA

GTTGACTAAACAGCGTCTGGTAGACGCAGATGGCATCATTAATCCGAGCGCTTTCTACATCTACCTGACC

GCTTGGGTCAGCAACGACCCTGTAGCTTACGCTGCCTCCCAGGCCAACATCCGGCCTCACCGGCCGGAGT

GGGTCCATGACAAAGCCGACTACATGCCAGAGACCAGGCTGAGAATCCCAGCAGCAGAGCCCATCGAGTA

CGCTCAGTTCCCTTTCTACCTCAACGGCCTACGAGACACCTCAGACTTTGTGGAAGCCATAGAAAAAGTG

AGAGTCATCTGTAACAACTATACGAGCCTGGGACTGTCCAGCTACCCCAATGGCTACCCCTTCCTGTTCT

GGGAGCAATACATCAGCCTGCGCCACTGGCTGCTGCTATCCATCAGCGTGGTGCTGGCCTGCACGTTTCT

AGTGTGCGCAGTCTTCCTCCTGAACCCCTGGACGGCCGGGATCATTGTCATGGTCCTGGCTCTGATGACC

GTTGAGCTCTTTGGCATGATGGGCCTCATTGGGATCAAGCTGAGTGCTGTGCCTGTGGTCATCCTGATTG

CATCTGTTGGCATCGGAGTGGAGTTCACCGTCCACGTGGCTTTGGCCTTTCTGACAGCCATTGGGGACAA

GAACCACAGGGCTATGCTCGCTCTGGAGCACATGTTTGCTCCCGTTCTGGACGGTGCTGTGTCCACTCTG

CTGGGTGTACTGATGCTTGCAGGGTCCGAATTTGATTTCATTGTCAGATACTTCTTTGCCGTCCTGGCCA

TTCTCACCGTCTTGGGGGTTCTCAATGGACTGGTTCTGCTGCCTGTCCTCTTATCCTTCTTTGGACCGTG

TCCTGAGGTGTCTCCAGCCAATGGCCTAAACCGACTGCCCACTCCTTCGCCTGAGCCGCCTCCAAGTGTC

GTCCGGTTTGCCGTGCCTCCTGGTCACACGAACAATGGGTCTGATTCCTCCGACTCGGAGTACAGCTCTC

AGACCACGGTGTCTGGCATCAGTGAGGAGCTCAGGCAATACGAAGCACAGCAGGGTGCCGGAGGCCCTGC

CCACCAAGTGATTGTGGAAGCCACAGAAAACCCTGTCTTTGCCCGGTCCACTGTGGTCCATCCGGACTCC

AGACATCAGCCTCCCTTGACCCCTCGGCAACAGCCCCACCTGGACTCTGGCTCCTTGTCCCCTGGACGGC

AAGGCCAGCAGCCTCGAAGGGATCCCCCTAGAGAAGGCTTGCGGCCACCCCCCTACAGACCGCGCAGAGA

CGCTTTTGAAATTTCTACTGAAGGGCATTCTGGCCCTAGCAATAGGGACCGCTCAGGGCCCCGTGGGGCC

CGTTCTCACAACCCTCGGAACCCAACGTCCACCGCCATGGGCAGCTCTGTGCCCAGCTACTGCCAGCCCA

TCACCACTGTGACGGCTTCTGCTTCGGTGACTGTTGCTGTGCATCCCCCGCCTGGACCTGGGCGCAACCC

CCGAGGGGGGCCCTGTCCAGGCTATGAGAGCTACCCTGAGACTGATCACGGGGTATTTGAGGATCCTCAT

GTGCCTTTTCATGTCAGGTGTGAGAGGAGGGACTCAAAGGTGGAGGTCATAGAGCTACAGGACGTGGAAT

GTGAGGAGAGGCCGTGGGGGAGCAGCTCCAACTGA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

References

 

1. Science 272:1668-1671(1996) 

2. Cancer Res. 56:4599-4601(1996)

3. J. Biol. Chem. 271:12125-12128(1996)

4. Hum. Genet. 100:497-502(1997)

5. Hum. Mutat. 11:480-480(1998) 

 

 

  

 

Additional supporting documents, including product datasheet, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.