CD56, also known as NCAM1 (Neural cell adhesion molecule 1) is a single pass type I transmembrane or GPI-anchored protein of the Ig superfamily (IgSF). There are 3 main forms of human CD56 that arise by alternate splicing. CD56 contains 2 fibronectin type-III motifs and 5 Ig-like C2-type motifs in the extracellular domain (ECD). CD56 may be involved in Ca++ independent cell matrix and homophilic or heterophilic cell-cell interactions.  CD56 is implicated in neural development and in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites. CD56 appears to be highly sialylated and the polysialyation reduces its adhesive property and increases its neurite outgrowth promoting features. CD56 specifically binds to heparan sulfate proteoglycans, the extracellular matrix protein agrin, and several chondroitin sulfate proteoglycans. CD56 has also been shown to be involved in the expansion of T cells, NK cells and dendritic cells which play an important role in immune surveillance.  CD56 also acts as a receptor for rabies virus. 

CD56, also known as NCAM1 (Neural cell adhesion molecule 1) is a single pass type I transmembrane or GPI-anchored protein of the Ig superfamily (IgSF). There are 3 main forms of human CD56 that arise by alternate splicing. CD56 contains 2 fibronectin type-III motifs and 5 Ig-like C2-type motifs in the extracellular domain (ECD). CD56 may be involved in Ca++ independent cell matrix and homophilic or heterophilic cell-cell interactions.  CD56 is implicated in neural development and in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites. CD56 appears to be highly sialylated and the polysialyation reduces its adhesive property and increases its neurite outgrowth promoting features. CD56 specifically binds to heparan sulfate proteoglycans, the extracellular matrix protein agrin, and several chondroitin sulfate proteoglycans. CD56 has also been shown to be involved in the expansion of T cells, NK cells and dendritic cells which play an important role in immune surveillance.  CD56 also acts as a receptor for rabies virus. 

 

Product Details

 

Gene Symbol: CD56; NCAM1; NCAM; N-CAM-1; NCAM-1; MSK39

 

NCBI Gene ID: 4684

 

Uniprot Entry: P13591

 

Construct Details: The recombinant human CD56-Fc fusion is expressed as a 926 amino acid protein consisting of Gln21 - Gly718 region of CD56 (UniProt#P13591) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.

   

Source: Human cells stably expressing human CD56-Fc and growing in chemical-defined media with no animal components or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60 

QVDIVPSQGE ISVGESKFFL CQVAGDAKDK DISWFSPNGE KLTPNQQRIS VVWNDDSSST 

        70         80         90        100        110        120 

LTIYNANIDD AGIYKCVVTG EDGSESEATV NVKIFQKLMF KNAPTPQEFR EGEDAVIVCD 

       130        140        150        160        170        180 

VVSSLPPTII WKHKGRDVIL KKDVRFIVLS NNYLQIRGIK KTDEGTYRCE GRILARGEIN 

       190        200        210        220        230        240 

FKDIQVIVNV PPTIQARQNI VNATANLGQS VTLVCDAEGF PEPTMSWTKD GEQIEQEEDD 

       250        260        270        280        290        300 

EKYIFSDDSS QLTIKKVDKN DEAEYICIAE NKAGEQDATI HLKVFAKPKI TYVENQTAME 

       310        320        330        340        350        360 

LEEQVTLTCE ASGDPIPSIT WRTSTRNISS EEKTLDGHMV VRSHARVSSL TLKSIQYTDA  

       370        380        390        400        410        420 

GEYICTASNT IGQDSQSMYL EVQYAPKLQG PVAVYTWEGN QVNITCEVFA YPSATISWFR 

       430        440        450        460        470        480 

DGQLLPSSNY SNIKIYNTPS ASYLEVTPDS ENDFGNYNCT AVNRIGQESL EFILVQADTP 

       490        500        510        520        530        540 

SSPSIDQVEP YSSTAQVQFD EPEATGGVPI LKYKAEWRAV GEEVWHSKWY DAKEASMEGI  

       550        560        570        580        590        600 

VTIVGLKPET TYAVRLAALN GKGLGEISAA SEFKTQPVQG EPSAPKLEGQ MGEDGNSIKV 

       610        620        630        640        650        660 

NLIKQDDGGS PIRHYLVRYR ALSSEWKPEI RLPSGSDHVM LKSLDWNAEY EVYVVAENQQ 

       670        680        690        700        710        720 

GKSKAAHFVF RTSAQPTAIP ANGSPTSGST TENLYFQGST GTHTCPPCPA PELLGGPSVF  

       730        740        750        760        770        780 

LFPPKPKDTL MISRTPEVTC VVVDVSHEDP EVKFNWYVDG VEVHNAKTKP REEQYNSTYR  

       790        800        810        820        830        840 

VVSVLTVLHQ DWLNGKEYKC KVSNKALPAP IEKTISKAKG QPREPQVYTL PPSREEMTKN 

       850        860        870        880        890        900 

QVSLTCLVKG FYPSDIAVEW ESNGQPENNY KTTPPVLDSD GSFFLYSKLT VDKSRWQQGN 

       910        920 

VFSCSVMHEA LHNHYTQKSL SLSPGK 

 

 

M.W.: Calculated molecular mass (kDa): 102.8; Estimated by SDS-PAGE under reducing condition (kDa): 100-110

 

Calculated PI: 5.02

 

Calculated extinction coefficients (M-1 cm-1, at 280nm): 141150

 

Purity: >90% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "DTT: +")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Supports the adhesion of mammalian cells such as neuroblastoma cells a dose dependent manner

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. Cell 50:1119 (1987)

2. J. Immunol. 146:4421 (1991)

3. Mol Cell Biol 11: 1654 (1991)

4. J. Neurosci. Res. 41:49 (1995)

5. J Biol. Chem.273:27124 (1998)

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: FCL2487B-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.