Human DcR1 Full-length Gene in Lentivector, Endotoxin-free DNA

Description

DcR1 ( decoy receptor 1), also known as CD263, TRAILR3, LIT and TRID, is a glycosyl­phosphatidylinositol (GPI)­linked membrane protein of the TNF receptor superfamily (TNFRSF10C).  DcR1 is highly expressed in peripheral blood lymphocytes, spleen, skeletal muscle, placenta, lung and heart. DcR1 is one of 5 receptors for TRAIL (tumor necrosis factor-related apoptosis inducing ligand), also known as Apo2L (Apo-2 ligand) and TNFSF10. TRAIL preferentially induces apoptosis in tumor cell lines, but not in normal cells. In addition to DcR2, DcR3 and OPG, DcR1 is another TNFRSF member that modulates cytotoxic ligands-induced apoptosis. DcR1 binds TRAIL with high affinity. However DcR1 lacks a cytoplasmic death domain and hence is not capable of inducing apoptosis. DcR1 may protect cells against TRAIL mediated apoptosis by competing with DR4/TRAILR1 and DR5/TRAILR2 for binding to the ligand. Expression of DcR1 has been shown to protect cells bearing DR4 and/or DR5 from TRAIL­induced apoptosis. DcR1 is also a p53-regulated DNA damage-inducible gene. DcR1 is detected in many normal tissues but not in most cancer cell lines, which may explain the specific sensitivity of cancer cells to the apoptosis-inducing activity of TRAIL. The soluble DcR1-Fc fusion protein neutralizes the ability of TRAIL to induce apoptosis.

  

 

Gene Symbol: DcR1; LIT; TRID; CD263; TRAILR3; TRAIL-R3; DCR1-TNFR; TNFRSF10C

  

NCBI Gene ID: 8794

 

Uniprot Entry: O14798

 

Construct Details: Full length human DcR1/CD263 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles.

 

Vector Type: pLTC (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Sequence:  

ATGGCCCGGATCCCCAAGACCCTAAAGTTCGTCGTCGTCATCGTCGCGGTCCTGCTGCCAGTCCTAGCTTACTCTGCCAC

CACTGCCCGGCAGGAGGAAGTTCCCCAGCAGACAGTGGCCCCACAGCAACAGAGGCACAGCTTCAAGGGGGAGGAGTGTC

CAGCAGGATCTCATAGATCAGAACATACTGGAGCCTGTAACCCGTGCACAGAGGGTGTGGATTACACCAACGCTTCCAAC

AATGAACCTTCTTGCTTCCCATGTACAGTTTGTAAATCAGATCAAAAACATAAAAGTTCCTGCACCATGACCAGAGACAC

AGTGTGTCAGTGTAAAGAAGGCACCTTCCGGAATGAAAACTCCCCAGAGATGTGCCGGAAGTGTAGCAGGTGCCCTAGTG

GGGAAGTCCAAGTCAGTAATTGTACGTCCTGGGATGATATCCAGTGTGTTGAAGAATTTGGTGCCAATGCCACTGTGGAA

ACCCCAGCTGCTGAAGAGACAATGAACACCAGCCCGGGGACTCCTGCCCCAGCTGCTGAAGAGACAATGAACACCAGCCC

GGGGACTCCTGCCCCAGCTGCTGAAGAGACAATGACCACCAGCCCGGGGACTCCTGCCCCAGCTGCTGAAGAGACAATGA

CCACCAGCCCGGGGACTCCTGCCCCAGCTGCTGAAGAGACAATGATCACCAGCCCGGGGACTCCTGCCTCTTCTCATTAC

CTCTCATGCACCATCGTAGGGATCATAGTTCTAATTGTGCTTCTGATTGTGTTTGTTTGA

  

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CGGGAAGCAATAGCATGATA-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

1. FEBS Lett. 416:329-334(1997).

2. J. Biol. Chem. 272:25417 (1997). 

3. J. Exp. Med. 186:1165 (1997).

4. Science 277:815 (1997). 

5. Science 277:818 (1997).

6. J. Immunol. 160:3 (1998).

 

 

 

 

 

Product datasheet (pdf) can be downloaded here: LTPSF71-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.