Human LTA/TNFSF1/TNFB/Lymphotoxin-alpha/LT-alpha Protein, Biotinylated, Recombinant

Description

LTA (lymphotoxin alpha) or TNFB (tumor necrosis factor beta) is a member of the tumor necrosis factor superfamily (TNFSF1). LTA/TNFSF1 is a cytokine produced by lymphocytes. LTA is highly inducible, secreted, and forms heterotrimers with LTB/TNFSF3 (lymphotoxin-beta), which anchor LTA/TNFSF1 to the cell surface. LTA/TNFSF1 also mediates a large variety of inflammatory, immunostimulatory, and antiviral responses, is involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis. Genetic variations in the LTA gene are associated with susceptibility to leprosy type 4, myocardial infarction, non-Hodgkin's lymphoma, and psoriatic arthritis. 

  

 

Gene Symbol: TNFSF1;  LTA; LT; TNFB; TNFSF-1; TNLG1E

  

NCBI Gene ID: 4049

 

Uniprot Entry: P01374

 

Construct Details: The recombinant human LTA protein  is expressed as a 192-amino acid protein consisting of Leu35 - Leu205aa region of LTA (UniProt accession #P01374) and a C-terminal His-tag.  It contains 1 potential N-linked glycosylation sites.

 

Source: Human cells stably expressing LTA and growing in chemical-defined media with no animal component or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60 

LPGVGLTPSA AQTARQHPKM HLAHSTLKPA AHLIGDPSKQ NSLLWRANTD RAFLQDGFSL  

        70         80         90        100        110        120 

SNNSLLVPTS GIYFVYSQVV FSGKAYSPKA TSSPLYLAHE VQLFSSQYPF HVPLLSSQKM  

       130        140        150        160        170        180 

VYPGLQEPWL HSMYHGAAFQ LTQGDQLSTH TDGIPHLVLS PSTVFFGAFA LSTTENLYFQ 

       190 

GSTGHHHHHH HH 

 

  

M.W.: Calculated molecular mass (kDa): 21.1; Estimated by SDS-PAGE under reducing condition (kDa): ~25-28

 

Calculated PI: 8.25

 

Calculated extinction coefficients (M-1 cm-1, at 280nm):  22920

  

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "S")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (concentration determined by UV spectrometry and verified by SDS-PAGE and Coomassie blue staining).  The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

 

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.