Human B7-H6 Full-length Gene in Lentivector, Endotoxin-free DNA

Product ID: pLTC-B7-H6 (SKU#: LTP1148)

Price:

$696.00

Description

B7H6 (B7 homolog 6), also known as NCR3LG1 (Natural cytotoxicity triggering receptor 3 ligand 1), is a single pass, type I transmembrane glycoprotein that belongs to the B7 family of the Immunoglobulin (Ig) superfamily. Among the family members, they share about 20-25% amino acid (aa) identity. Like other B7 family members, B7-H6 contains one Ig V-like and one Ig C-like domain in the extracellular region. Within the ECD, human B7H6 shares 99% aa identity with chimpanzee B7H6 and 53% - 56% with bovine, canine, and equine B7H6. Orthologs in rodents have not been identified. The Ig V-like domain of B7-H6 mediates the interaction with NKp30 (also known as NCR3 and CD337) expressed on NK cells, resulting in natural killer (NK) cell activation and cytotoxicity. B7-H6 does not show binding to other natural killer cell-activating receptors, such as NKp44, NKp46, or NKG2D. Ligation of NKp30 by B7H6 induces NK cell activation and target cell cytolysis. B7-H6 is expressed on a wide range of tumor cells, including T and B-lymphomas, myeloid leukemias, melanomas, carcinomas and large T SV40 antigen-transformed cells, The B7-H6 expression is consistent with the detection of NKp30 binding sites on these tumor cells and correlates with tumor cell sensitivity to NKp30-dependent cell lysis.

Gene Symbol: B7-H6; B7H6; NCR3LG1; DKFZp686O24166

NCBI Gene ID: 374383

Uniprot Entry: Q68D85

Construct Details: Full length human B7-H6 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter, which can be used for both transient and stable expression in mammalian cells. It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles.

Vector Type: pLTC (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

Gene Insert Size: 1365 (bp)

Gene Insert Sequence:

ATGACGTGGAGGGCTGCCGCCTCCACGTGCGCGGCGCTCCTGATTCTGCTGTGGGCGCTGACGACCGAAGGTGATCTGAA

AGTAGAGATGATGGCAGGGGGGACTCAGATCACACCCCTGAATGACAATGTCACCATATTCTGCAATATCTTTTATTCCC

AACCCCTCAACATCACGTCTATGGGTATCACCTGGTTTTGGAAGAGTCTGACGTTTGACAAAGAAGTCAAAGTCTTTGAA

TTTTTTGGAGATCACCAAGAGGCATTCCGACCTGGAGCCATTGTGTCTCCATGGAGGCTGAAGAGTGGGGACGCCTCACT

GCGGCTGCCTGGAATCCAGCTGGAGGAAGCAGGAGAGTACCGATGTGAGGTGGTGGTCACCCCTCTGAAGGCACAGGGAA

CAGTCCAGCTTGAAGTTGTGGCTTCCCCAGCCAGCAGATTGTTGCTGGATCAAGTGGGCATGAAAGAGAATGAAGACAAA

TATATGTGTGAGTCAAGTGGGTTCTACCCAGAGGCTATTAATATAACATGGGAGAAGCAGACCCAGAAGTTTCCCCATCC

CATAGAGATTTCTGAGGATGTCATCACTGGTCCCACCATCAAGAATATGGATGGCACATTTAATGTCACTAGCTGCTTGA

AGCTGAACTCCTCTCAGGAAGACCCTGGGACTGTCTACCAGTGTGTGGTACGGCATGCGTCCTTGCATACCCCCTTGAGG

AGCAACTTTACCCTGACTGCTGCTCGGCACAGTCTTTCTGAAACTGAGAAGACAGATAATTTTTCCATTCATTGGTGGCC

TATTTCATTCATTGGTGTTGGACTGGTTTTATTAATTGTTTTGATTCCTTGGAAAAAGATATGTAACAAATCATCTTCAG

CCTATACTCCTCTCAAGTGCATTCTGAAACACTGGAACTCCTTTGACACTCAGACTCTGAAGAAAGAGCACCTCATATTC

TTTTGCACTCGGGCATGGCCGTCTTACCAGCTGCAGGATGGGGAGGCTTGGCCTCCTGAGGGAAGTGTTAATATTAATAC

TATTCAACAACTAGATGTTTTCTGCAGACAGGAGGGCAAATGGTCCGAGGTTCCTTATGTGCAAGCCTTCTTTGCCTTGC

GAGACAACCCAGATCTTTGTCAGTGTTGTAGAATTGACCCTGCTCTCCTAACAGTTACATCAGGCAAGTCCATAGATGAT

AATTCCACAAAGTCTGAGAAACAAACCCCTAGGGAACACTCGGATGCAGTTCCGGATGCCCCAATCCTTCCTGTCTCCCC

TATCTGGGAACCTCCTCCAGCCACAACATCAACAACTCCAGTTCTATCCTCCCAACCCCCAACTTTACTGTTACCCCTAC

AGTAA

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CGGGAAGCAATAGCATGATA-3’

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification. High fidelity PCR systems should always be used.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

1. J. Exp. Med. 206:1495 (2009).

2. J. Exp. Med. 208:703 (2011).

3. Proc. Natl. Acad. Sci. 108:6223 (2011).

4. Immunol. Rev. 241:180 (2011).

5. J Exp Med. 11;208:703 (2011).

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Product datasheet (pdf) can be downloaded here: LTP1148-PDS.pdf