Human CD226/DNAM1 Full-length Gene in Lentivector, Endotoxin-free DNA

Description

The leukocyte adhesion molecule CD226, also known as DNAM1 (DNAX accessory molecule­1) and PTA1 (platelet and T cell activation antigen 1), is a single-pass type I transmembrane glycoprotein belonging to the immunoglobulin (Ig) superfamily (IgSF). CD226 contains a 236 amino acid (aa) extracellular domain (ECD) with 2 Ig­like C2­set (IgC2) domains and a 61 aa cytoplasmic region that contains motifs for binding PDZ domains and band 4.1 family proteins. CD226 is identified as an intercellular adhesion molecule, which is constitutively expressed on the majority of CD4+ and CD8+ T lymphocytes, natural killer (NK) cells, monocytes/macrophages, and a subset of B lymphocytes. CD226 is expressed on multiple lymphoid and myeloid cells and interacts with the poliovirus receptor CD155/PVR and its family member CD112/Nectin­2. Ligation of CD226 promotes the activation of NK cells, CD8+ T cells, and mast cells, dendritic cell maturation, megakaryocyte and activated platelet adhesion to vascular endothelial cells, and monocyte extravasation; but inhibits the forrmation of osteoclasts. CD226 is critical for NK cell- and CD8+ T cell-mediated cytotoxicity.  It also plays an important role in immune surveillance of tumor development and is involved in triggering naive T cell differentiation and proliferation.  Platelet­endothelium interactions mediated by CD226 may contribute to the metastasis of tumor cells to the lung.

 

 

Gene Symbol: CD226; PTA1; DNAM1; DNAM-1; TLiSA1

 

NCBI Gene ID: 10666

 

Uniprot Entry: Q15762

 

Construct Details: Full length human CD226 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles.

 

Vector Type: pLTC (lentiviral expression vector containing a heterologous CMV promoter with or without a selection marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1011 (bp)

 

Gene Insert Sequence:  

ATGGATTATCCTACTTTACTTTTGGCTCTTCTTCATGTATACAGAGCTCTATGTGAAGAGGTGCTTTGGCATACATCAGT

TCCCTTTGCCGAGAACATGTCTCTAGAATGTGTGTATCCATCAATGGGCATCTTAACACAGGTGGAGTGGTTCAAGATCG

GGACCCAGCAGGATTCCATAGCCATTTTCAGCCCTACTCATGGCATGGTCATAAGGAAGCCCTATGCTGAGAGGGTTTAC

TTTTTGAATTCAACGATGGCTTCCAATAACATGACTCTTTTCTTTCGGAATGCCTCTGAAGATGATGTTGGCTACTATTC

CTGCTCTCTTTACACTTACCCACAGGGAACTTGGCAGAAGGTGATACAGGTGGTTCAGTCAGATAGTTTTGAGGCAGCTG

TGCCATCAAATAGCCACATTGTTTCGGAACCTGGAAAGAATGTCACACTCACTTGTCAGCCTCAGATGACGTGGCCTGTG

CAGGCAGTGAGGTGGGAAAAGATCCAGCCCCGTCAGATCGACCTCTTAACTTACTGCAACTTGGTCCATGGCAGAAATTT

CACCTCCAAGTTCCCAAGACAAATAGTGAGCAACTGCAGCCACGGAAGGTGGAGCGTCATCGTCATCCCCGATGTCACAG

TCTCAGACTCGGGGCTTTACCGCTGCTACTTGCAGGCCAGCGCAGGAGAAAACGAAACCTTCGTGATGAGATTGACTGTA

GCCGAGGGTAAAACCGATAACCAATATACCCTCTTTGTGGCTGGAGGGACAGTTTTATTGTTGTTGTTTGTTATCTCAAT

TACCACCATCATTGTCATTTTCCTTAACAGAAGGAGAAGGAGAGAGAGAAGAGATCTATTTACAGAGTCCTGGGATACAC

AGAAGGCACCCAATAACTATAGAAGTCCCATCTCTACCGGTCAACCTACCAATCAATCCATGGATGATACAAGAGAGGAT

ATTTATGTCAACTATCCAACCTTCTCTCGCAGACCAAAGACTAGAGTTTAA

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) Reverse (or 3'-end) primer: 5’-CGGGAAGCAATAGCATGATA-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

1. Immunity 4:573 (1996)

2. J. Immunol. 166:1671 (1998)

3. J. Exp. Med. 198:557 (2003)

4. J. Biol. Chem. 278:36748 (2003)

5. J. Immunol. 175:1558 (2005)

6. Blood 107:1491 (2006)

 

 

 

Product datasheet (pdf) can be downloaded here: LTP0964-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.