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Human CD27 Protein, ECD (Extracellular Domain), Fc-fusion, Biotinylated, Recombinant

Product ID: CD27-Fc-Biot (SKU#: FCL0102B)

Price:

$628.00

Description

CD27 is a single-pass, type I transmembrane glycoprotein in the TNF receptor superfamily (TNFRSF7). CD27 is expressed as a disulfidelinked homodimer on the cell surface. Proteolytic cleavage of CD27 results in the shedding of a 2832 kDa fragment of the extracellular domain (ECD) with three TNFR cysteinerich repeats. The expression of CD27 is limited to cells of the lymphoid lineage. CD27 is weakly expressed on naïve T cells and NK cells and is upregulated upon cell activation. It is also upregulated on activated germinal center B cells, plasma cells, and a subset of memory B cells. CD27 acts as a costimulatory molecule that supports lymphocyte activation and survival. As a T and B cell co-stimulatory molecule, the activity of CD27 is governed by a TNF-like ligand CD70 on lymphocytes and dendritic cells. CD27 binds to the transmembrane glycoprotein CD70 that is expressed on activated B cells, activated T cells, and dendritic cells. CD27 transduces signals and subsequently leads to the activation of NF-κB and JNK pathways that contribute to the activation and survival of T cells and NK cells as well as the long-term maintenance of T cell immunity. CD27 also plays a key role in regulating B-cell differentiation, activation and immunoglobulin synthesis. Ligation of CD27 on B cells promotes germinal center formation and the expansion and affinity maturation of memory B cell responses. Mutations in the CD27 gene are associated with an autosomal recessive immunodeficiency disorder, lymphoproliferative syndrome 2 (LPFS2).

Gene Symbol: TNFRSF7; T14; S152; Tp55; S152. LPFS2

NCBI Gene ID: 939

Uniprot Entry: P26842

Construct Details: The recombinant human CD27-Fc fusion is expressed as a 400 amino acid protein consisting of Ala20 - Arg191 region of CD27 (UniProt accession #P26842) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions (see the gel image inserted above).

Source: Human cells stably expressing human CD27-Fc and growing in chemical-defined media with no animal components or antibiotics

Deduced Amino Acid Sequence:

10 20 30 40 50 60

ATPAPKSCPE RHYWAQGKLC CQMCEPGTFL VKDCDQHRKA AQCDPCIPGV SFSPDHHTRP

70 80 90 100 110 120

HCESCRHCNS GLLVRNCTIT ANAECACRNG WQCRDKECTE CDPLPNPSLT ARSSQALSPH

130 140 150 160 170 180

PQPTHLPYVS EMLEARTAGH MQTLADFRQL PARTLSTHWP PQRSLCSSDF IRSTGTHTCP

190 200 210 220 230 240

PCPAPELLGG PSVFLFPPKP KDTLMISRTP EVTCVVVDVS HEDPEVKFNW YVDGVEVHNA

250 260 270 280 290 300

KTKPREEQYN STYRVVSVLT VLHQDWLNGK EYKCKVSNKA LPAPIEKTIS KAKGQPREPQ

310 320 330 340 350 360

VYTLPPSREE MTKNQVSLTC LVKGFYPSDI AVEWESNGQP ENNYKTTPPV LDSDGSFFLY

370 380 390 400

SKLTVDKSRW QQGNVFSCSV MHEALHNHYT QKSLSLSPGK

M.W.: Calculated molecular mass (kDa): 44.8; Estimated by SDS-PAGE under reducing condition (kDa): 65-70

Calculated PI: 7.81

Calculated extinction coefficients (M-1 cm-1, at 280nm): 56265

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "DTT: +")

Formulation: Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

Biological Activity: Binds to its ligand CD70 and inhibits CD70-mediated signaling activities including proliferation of T cells induced by CD70 and antiCD3 antibody.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

1. J. Immunol. 139:1589 (1987).

2. Cell 73:447 (1993).

3. J. Immunol. 152:1756 (1994).

4. Proc. Natl. Acad. Sci. 92: 11249 (1995).

5. Immunol. Rev. 229:216 (2009).

Product datasheet (pdf) can be downloaded here: FCL0102B-PDS.pdf