Mouse Fc (IgG2a) Control Protein, Biotinylated, Recombinant

Description

The mus musculus or mouse IGHG (immunoglobulin heavy constant gamma) gene encodes the Immunoglobulin (Ig) heavy chain gamma polypeptide or Ig gamma-2A chain C region, A allele. The C region contains 3 Ig-like domains including the CH1, hinge, CH2 and CH3 domains.  The hinge, CH2 and CH3 region is also commonly referred to as the Fc region.  Fc meditates the interactions with the complement components and Fc-gamma receptors (FCGRs) to induce innate immune responses such as antibody-dependent complement activation and phagocytosis, respectively. The mouse Fc control protein is expressed as a recombinant protein that include the hinge , CH2 and CH3 domains of mouse Ig gamma-2a chain C region (which can be used for making mouse Fc-fusion proteins).  The purified protein can be used as a negative control for mouse  Fc-fusion proteins in a variety of biological assays. 

 

 

Gene Symbol: IGHG; IGHG2A; AU044919

 

NCBI Gene ID: 380794

 

Uniprot Entry: P01863

 

Construct Details: The recombinant mouse Fc control protein is expressed as a 233 amino acid protein consisting of Glu98 - Lys330 region of mouse IGHG or Ig gamma-2A chain C region, A allele (UniProt accession #P01863), which exists as a dimer under non-reducing condition (see the gel image inserted).

 

Source:  Human cells stably expressing mouse Fc and growing in chemical-defined media with no animal components or antibiotics

 

Deduced Amino Acid Sequence:

        10         20         30         40         50         60 

EPRGPTIKPC PPCKCPAPNL LGGPSVFIFP PKIKDVLMIS LSPIVTCVVV DVSEDDPDVQ 

        70         80         90        100        110        120 

ISWFVNNVEV HTAQTQTHRE DYNSTLRVVS ALPIQHQDWM SGKEFKCKVN NKDLPAPIER 

       130        140        150        160        170        180 

TISKPKGSVR APQVYVLPPP EEEMTKKQVT LTCMVTDFMP EDIYVEWTNN GKTELNYKNT 

       190        200        210        220        230 

EPVLDSDGSY FMYSKLRVEK KNWVERNSYS CSVVHEGLHN HHTTKSFSRT PGK 

 

M.W.:  Calculated molecular mass 26.4 kDa; estimated by SDS-PAGE under reducing condition 30-35 kDa probably due to glycosylation

 

Calculated PI: 6.74

 

Calculated Extinction Coefficients (M-1 cm-1, at 280nm): 32805

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above)

 

Formulation: Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier free & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

  

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks.

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

1. Eur. J. Biochem. 43:423 (1974).

2. Nucleic Acids Res. 8:3143 (1980).

3. Nucleic Acids Res. 9:1365 (1981).

4. Proc. Natl. Acad. Sci. 78:2442 (1981).

 

 

 

 

 FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

Product datasheet (pdf) can be downloaded here: FCL0121B-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.