Human B7-H3b/4Ig-B7-H3 Protein, ECD, Fc-fusion, Recombinant

Product ID: B7-H3b-Fc (SKU#: FCL1662)

Price:

$608.00

Description

B7H3 (B7 homolog 3), also known as CD276, is a single pass type I transmembrane glycoprotein that belongs to the B7 family of the Ig superfamily. Like other B7 family members, B7-H3 contains one Ig V-like and one Ig C-like domain in the extracellular region. Among the family members, they share about 20-25% amino acid identity. B7-H3 serves as an accessory modulator of T-cell response. It has been reported that B7-H3 can provide both a stimulatory and inhibitory signal to T cells. As a negative regulator, it preferentially affects Th1 responses. B7-H3 may also play an important role in muscle-immune interactions. A longer form of B7-H3 has been identified in human but not in mouse. It is termed 4IgB7H3 or B7H3b with two additional Iglike domains (one V-type and one C-type). B7-H3b appears to be the dominantly expressed form in human and is found on human dendritic cells, activated T, B and NK cells. Human B7H3b binding to an undefined receptor may inhibit NK cell killing and cytokine release. It is also involved in late stage osteoblast differentiation. B7-H3 is detected in a variety of cancer types and its expression in some cancers correlates with poor outcome of cancer patients.

Gene Symbol: B7-H3; CD276; B7H3; B7RP-2; 4Ig-B7-H3; PSEC0249; UNQ309/PRO352

NCBI Gene ID: 80381

Uniprot Entry: Q5ZPR3

Construct Details: The recombinant human B7-H3b-Fc fusion is expressed as a 666 amino acid protein consisting of Leu29 - Ala466 region of B7-H3b or 4IgB7-H3 (UniProt accession #Q5ZPR3 - isoform 1) and a C-terminal Fc fusion from human IgG1, which exists as a dimer under non-reducing condition

Source: Human cells stably expressing B7-H3b-Fc and growing in chemical-defined media with no animal components or antibiotics

Deduced Amino Acid Sequence:

10 20 30 40 50 60

LEVQVPEDPV VALVGTDATL CCSFSPEPGF SLAQLNLIWQ LTDTKQLVHS FAEGQDQGSA

70 80 90 100 110 120

YANRTALFPD LLAQGNASLR LQRVRVADEG SFTCFVSIRD FGSAAVSLQV AAPYSKPSMT

130 140 150 160 170 180

LEPNKDLRPG DTVTITCSSY QGYPEAEVFW QDGQGVPLTG NVTTSQMANE QGLFDVHSIL

190 200 210 220 230 240

RVVLGANGTY SCLVRNPVLQ QDAHSSVTIT PQRSPTGAVE VQVPEDPVVA LVGTDATLRC

250 260 270 280 290 300

SFSPEPGFSL AQLNLIWQLT DTKQLVHSFT EGRDQGSAYA NRTALFPDLL AQGNASLRLQ

310 320 330 340 350 360

RVRVADEGSF TCFVSIRDFG SAAVSLQVAA PYSKPSMTLE PNKDLRPGDT VTITCSSYRG

370 380 390 400 410 420

YPEAEVFWQD GQGVPLTGNV TTSQMANEQG LFDVHSVLRV VLGANGTYSC LVRNPVLQQD

430 440 450 460 470 480

AHGSVTITGQ PMTFPPEAST GTHTCPPCPA PELLGGPSVF LFPPKPKDTL MISRTPEVTC

490 500 510 520 530 540

VVVDVSHEDP EVKFNWYVDG VEVHNAKTKP REEQYNSTYR VVSVLTVLHQ DWLNGKEYKC

550 560 570 580 590 600

KVSNKALPAP IEKTISKAKG QPREPQVYTL PPSREEMTKN QVSLTCLVKG FYPSDIAVEW

610 620 630 640 650 660

ESNGQPENNY KTTPPVLDSD GSFFLYSKLT VDKSRWQQGN VFSCSVMHEA LHNHYTQKSL

SLSPGK

M.W.: Calculated molecular mass 72.6 kDa; estimated by SDS-PAGE under reducing condition ~95 kDa and ~65 kDa probably representing the 4Ig-B7-H3 and 2Ig-B7-H3 isoforms

Calculated PI: 5.29

Calculated Extinction Coefficients (M-1 cm-1, at 280nm): 73185

Purity:>95% judged by SDS-PAGE under reducing condition with two major products: 25% of 95 kDa and 75% of 65 kDa (see the gel image above)

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier free).

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

Biological Activity: Inhibits antiCD3induced proliferation of stimulated human T cells. Binds anti-B7-H3 monoclonal antibodies, human IgG1 (SKU#MAB1755) and rabbit IgG (SKU#MAB1753) in a functional ELISA (see Technical Data)

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

Recombinant B7-H3b-Fc protein (SKU#FCL1662) was immobilized at 50 ng/well on a 96-well NuncMaxisorp plate for ELISA with serially diluted biotinylated anti-B7-H3/CD276 monoclonal antibody, human IgG1 (SKU#MAB1755B). Biotinylated anti-OX40 (SKU#MAB0103B) human IgG1 was used as a control to demonstrate the specificity of B7-H3b binding under the experimental conditions. The binding curve was fit and apparent K_D was calculated for anti-B7-H3:B7-H3b binding to be 152 ± 8 pM.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

1. Nat. Immunol. 2:269 (2001).

2. Nat. Immunol. 2:203 (2001).

3. J. Immunol. 168:6294 (2002).

4. J. Immunol. 172:2352 (2004).

5. Proc. Natl. Acad. Sci. 101:12969 (2004).

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Product datasheet (pdf) can be downloaded here: FCL1662-PDS.pdf