Human NPR2/ANPRB/GC-B/GUC2B/GUCY2B/AMDM/ECDM Protein, ECD(Extracellular Domain), Fc-fusion, Recombinant

NPR2 (natriuretic peptide receptor 1), also known as GC-B, ANPRB and GUCY2B, is a single pass type I transmembrane glycoprotein that belongs to the adenylyl cyclase class-4/guanylyl cyclase family. Guanylyl cyclases catalyze the production of cGMP from GTP and exist as soluble and membrane forms.  The membrane guanylyl cyclases, often termed guanylyl cyclases A through F, are a family of cell-surface receptors with a similar topographic structure: an extracellular domain (ECD) for ligand-binding, a transmembrane (TM) domain, and an intracellular region that contains a protein kinase-like domain, a helical hinge region involved in oligomerization, and a carboxyl-terminal guanylyl cyclase catalytic domain. NPR2 is a membrane-bound guanylate cyclase that serves as the primary receptor for the C-type natriuretic peptide NPPC/CNP hormone. NPR2 may play a role in the regulation of skeletal growth. The mutations in the NPR2 gene are associated with AMD1 (acromesomelic dysplasia 1), a skeletal disorder characterized by short stature, very short limbs and hand/foot malformations, and ECDM (epiphyseal chondrodysplasia, miura type), an overgrowth syndrome characterized by tall stature, long hands and feet.

Gene Symbol: NPR2; ANPRB; GCB; AMDM; ANPb; ECDM; GC-B; NPRB; SNSK; GUC2B; NPRBi; GUCY2B

NCBI Gene ID: 4882

Uniprot Entry: P20594

Construct Details: The recombinant human NGFR-Fc fusion is expressed as a 659 amino acid protein consisting of Arg23 -Thr455 region of NPR2 and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions. 

Source: Human cells stably expressing NPR2-Fc and growing in chemical-defined media with no animal component or antibiotics

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60
RNLTLAVVLP EHNLSYAWAW PRVGPAVALA VEALGRALPV DLRFVSSELE GACSEYLAPL

        70         80         90        100        110        120
SAVDLKLYHD PDLLLGPGCV YPAASVARFA SHWRLPLLTA GAVASGFSAK NDHYRTLVRT

       130        140        150        160        170        180
GPSAPKLGEF VVTLHGHFNW TARAALLYLD ARTDDRPHYF TIEGVFEALQ GSNLSVQHQV

       190        200        210        220        230        240
YAREPGGPEQ ATHFIRANGR IVYICGPLEM LHEILLQAQR ENLTNGDYVF FYLDVFGESL

       250        260        270        280        290        300
RAGPTRATGR PWQDNRTREQ AQALREAFQT VLVITYREPP NPEYQEFQNR LLIRAREDFG

       310        320        330        340        350        360
VELGPSLMNL IAGCFYDGIL LYAEVLNETI QEGGTREDGL RIVEKMQGRR YHGVTGLVVM

       370        380        390        400        410        420
DKNNDRETDF VLWAMGDLDS GDFQPAAHYS GAEKQIWWTG RPIPWVKGAP PSDNPPCAFD

       430        440        450        460        470        480
LDDPSCDKTP LSTGTHTCPP CPAPELLGGP SVFLFPPKPK DTLMISRTPE VTCVVVDVSH

       490        500        510        520        530        540
EDPEVKFNWY VDGVEVHNAK TKPREEQYNS TYRVVSVLTV LHQDWLNGKE YKCKVSNKAL

       550        560        570        580        590        600
PAPIEKTISK AKGQPREPQV YTLPPSREEM TKNQVSLTCL VKGFYPSDIA VEWESNGQPE

       610        620        630        640        650
NNYKTTPPVL DSDGSFFLYS KLTVDKSRWQ QGNVFSCSVM HEALHNHYTQ KSLSLSPGK  

M.W.: Calculated molecular mass 73.6 kDa; estimated by SDS-PAGE under reducing condition: ~75-80 kDa 

Calculated PI: 5.79

Calculated extinction coefficients (M-1 cm-1, at 280nm):  110240

Purity: >90% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "S")

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (concentration determined by UV spectrometry and verified by SDS-PAGE and Coomassie blue staining).  

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Additional supporting documents, including PDS, COA and MSDS are available upon request.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.