Human CDH17/HPT1/Cadherin17 Protein, ECD (Extracellular Domain), Fc-fusion, Biotinylated, Recombinant

Cadherins are calcium-dependent cell adhesion proteins that preferentially interact with themselves in a homophilic manner in connecting cells.  Cadherins thus contribute to the sorting of heterogeneous cell types.  Cadherin17 or CDH17 is an 832 amino acid (aa) single pass type I transmembrane protein of the cadherin superfamily.  CDH17 contains an N-terminal signal peptide, a 765-aa extracellular domain (ECD) with 7 cadherin domains and 8 potential glycosylation sites, a transmembrane domain (TM), and a 24-aa short cytoplasmic tail. CDH17 is expressed in the gastrointestinal tract and pancreatic duct.  CDH17 is a component of the gastrointestinal tract and pancreatic ducts, acting as an intestinal proton-dependent peptide transporter in the first step in oral absorption of many medically important peptide-based drugs. CDH17 may also play a role in the morphological organization of liver and intestine. 

Gene Symbol: CDH17; Cadherin-17; LI-cadherin; HPT1; HPT-1; Cadherin17

NCBI Gene ID: 1015

Uniprot Entry: Q12864

Construct Details: The recombinant human CDH17-Fc is expressed as a 991 amino acid protein consisting of Gln23 - Val785 region of CD99 (UniProt #P14209 - isoform 1) and a C-terminal Fc fusion from human IgG1

Source: Human cells stably expressing CDH17-Fc and growing in chemical-defined media with no animal component or antibiotics

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60
QEGKFSGPLK PMTFSIYEGQ EPSQIIFQFK ANPPAVTFEL TGETDNIFVI EREGLLYYNR

        70         80         90        100        110        120
ALDRETRSTH NLQVAALDAN GIIVEGPVPI TIKVKDINDN RPTFLQSKYE GSVRQNSRPG

       130        140        150        160        170        180
KPFLYVNATD LDDPATPNGQ LYYQIVIQLP MINNVMYFQI NNKTGAISLT REGSQELNPA

       190        200        210        220        230        240
KNPSYNLVIS VKDMGGQSEN SFSDTTSVDI IVTENIWKAP KPVEMVENST DPHPIKITQV

       250        260        270        280        290        300
RWNDPGAQYS LVDKEKLPRF PFSIDQEGDI YVTQPLDREE KDAYVFYAVA KDEYGKPLSY

       310        320        330        340        350        360
PLEIHVKVKD INDNPPTCPS PVTVFEVQEN ERLGNSIGTL TAHDRDEENT ANSFLNYRIV

       370        380        390        400        410        420
EQTPKLPMDG LFLIQTYAGM LQLAKQSLKK QDTPQYNLTI EVSDKDFKTL CFVQINVIDI

       430        440        450        460        470        480
NDQIPIFEKS DYGNLTLAED TNIGSTILTI QATDADEPFT GSSKILYHII KGDSEGRLGV

       490        500        510        520        530        540
DTDPHTNTGY VIIKKPLDFE TAAVSNIVFK AENPEPLVFG VKYNASSFAK FTLIVTDVNE

       550        560        570        580        590        600
APQFSQHVFQ AKVSEDVAIG TKVGNVTAKD PEGLDISYSL RGDTRGWLKI DHVTGEIFSV

       610        620        630        640        650        660
APLDREAGSP YRVQVVATEV GGSSLSSVSE FHLILMDVND NPPRLAKDYT GLFFCHPLSA

       670        680        690        700        710        720
PGSLIFEATD DDQHLFRGPH FTFSLGSGSL QNDWEVSKIN GTHARLSTRH TEFEEREYVV

       730        740        750        760        770        780
LIRINDGGRP PLEGIVSLPV TFCSCVEGSC FRPAGHQTGI PTVSTGTHTC PPCPAPELLG

       790        800        810        820        830        840
GPSVFLFPPK PKDTLMISRT PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN AKTKPREEQY

       850        860        870        880        890        900
NSTYRVVSVL TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI SKAKGQPREP QVYTLPPSRE

       910        920        930        940        950        960
EMTKNQVSLT CLVKGFYPSD IAVEWESNGQ PENNYKTTPP VLDSDGSFFL YSKLTVDKSR

       970        980        990
WQQGNVFSCS VMHEALHNHY TQKSLSLSPG K

M.W.: Calculated molecular mass 111 kDa; estimated by SDS-PAGE under reducing condition 80-110 kDa (2 major bands probably due to proteolytic cleavage).

Calculated PI: 5.12

Calculated extinction coefficients (M-1 cm-1, at 280nm):  96900

Purity: >90% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "S")

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (concentration determined by UV spectrometry and verified by SDS-PAGE and Coomassie blue staining).  The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Additional supporting documents, including PDS, COA and MSDS are available upon request.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.