Human IL18R1/CD218a/IL1RRP Gene, Full-length cDNA in Lentivector, Endotoxin-free DNA

Description

IL18R1 (interleukin-18 receptor 1), also known as IL18RA, IL1RRP and CD218a, is a single-pass, type I transmembrane glycoprotein that belongs to the interleukin-1 receptor (IL1R) family. Like other family members, IL18R1 contain 3 immunoglobulin (Ig)-like domains in the extracellular region and an intracellular TIR (Toll-like receptor/IL-1 receptor) signaling domain that is also conserved in the Toll-like receptor family. Within the IL18 receptor complex, IL18R1 is responsible for the binding of the proinflammatory cytokine IL18, but not IL1A nor IL1B and contributes to IL18-induced cytokine production, either independently of SLC12A3, or as a complex with SLC12A3.  IL18 is a proinflammatory cytokine involved in inducing cell-mediated immunity. IFN-alpha and IL12 are reported to induce the expression of IL18R1 in NK and T cells. IL18R1 along with four other members of the interleukin 1 receptor family, including IL1R2, IL1R1, ILRL2 (IL-1Rrp2), and IL1RL1 (T1/ST2), form a gene cluster on chromosome 2q. 

 

 

Gene Symbol: IL18R1; CD218a; IL18RA; IL1RRP; CDw218a; IL-1Rrp

 

NCBI Gene ID: 8809

 

Uniprot Entry: Q13478

 

Construct Details: Full length human IL18R1 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter -/+ an antibiotic selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles.

 

Vector Type: pLTC or pLTC-Marker (see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1626 (bp)

 

Gene Insert Sequence:  

ATGAATTGTAGAGAATTACCCTTGACCCTTTGGGTGCTTATATCTGTAAGCACTGCAGAATCTTGTACTT

CACGTCCCCACATTACTGTGGTTGAAGGGGAACCTTTCTATCTGAAACATTGCTCGTGTTCACTTGCACA

TGAGATTGAAACAACCACCAAAAGCTGGTACAAAAGCAGTGGATCACAGGAACATGTGGAGCTGAACCCA

AGGAGTTCCTCGAGAATTGCTTTGCATGATTGTGTTTTGGAGTTTTGGCCAGTTGAGTTGAATGACACAG

GATCTTACTTTTTCCAAATGAAAAATTATACTCAGAAATGGAAATTAAATGTCATCAGAAGAAATAAACA

CAGCTGTTTCACTGAAAGACAAGTAACTAGTAAAATTGTGGAAGTTAAAAAATTTTTTCAGATAACCTGT

GAAAACAGTTACTATCAAACACTGGTCAACAGCACATCATTGTATAAGAACTGTAAAAAGCTACTACTGG

AGAACAATAAAAACCCAACGATAAAGAAGAACGCCGAGTTTGAAGATCAGGGGTATTACTCCTGCGTGCA

TTTCCTTCATCATAATGGAAAACTATTTAATATCACCAAAACCTTCAATATAACAATAGTGGAAGATCGC

AGTAATATAGTTCCGGTTCTTCTTGGACCAAAGCTTAACCATGTTGCAGTGGAATTAGGAAAAAACGTAA

GGCTCAACTGCTCTGCTTTGCTGAATGAAGAGGATGTAATTTATTGGATGTTCGGGGAAGAAAATGGATC

GGATCCTAATATACATGAAGAGAAAGAAATGAGAATTATGACTCCAGAAGGCAAATGGCATGCTTCAAAA

GTATTGAGAATTGAAAATATTGGTGAAAGCAATCTAAATGTTTTATATAATTGCACTGTGGCCAGCACGG

GAGGCACAGACACCAAAAGCTTCATCTTGGTGAGAAAAGCAGACATGGCTGATATCCCAGGCCACGTCTT

CACAAGAGGAATGATCATAGCTGTTTTGATCTTGGTGGCAGTAGTGTGCCTAGTGACTGTGTGTGTCATT

TATAGAGTTGACTTGGTTCTATTTTATAGACATTTAACGAGAAGAGATGAAACATTAACAGATGGAAAAA

CATATGATGCTTTTGTGTCTTACCTAAAAGAATGCCGACCTGAAAATGGAGAGGAGCACACCTTTGCTGT

GGAGATTTTGCCCAGGGTGTTGGAGAAACATTTTGGGTATAAGTTATGCATATTTGAAAGGGATGTAGTG

CCTGGAGGAGCTGTTGTTGATGAAATCCACTCACTGATAGAGAAAAGCCGAAGACTAATCATTGTCCTAA

GTAAAAGTTATATGTCTAATGAGGTCAGGTATGAACTTGAAAGTGGACTCCATGAAGCATTGGTGGAAAG

AAAAATTAAAATAATCTTAATTGAATTTACACCTGTTACTGACTTCACATTCTTGCCCCAATCACTAAAG

CTTTTGAAATCTCACAGAGTTCTGAAGTGGAAGGCCGATAAATCTCTTTCTTATAACTCAAGGTTCTGGA

AGAACCTTCTTTACTTAATGCCTGCAAAAACAGTCAAGCCAGGTAGAGACGAACCGGAAGTCTTGCCTGT

TCTTTCCGAGTCTTAA

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.