Human TLR5/SLE1/TIL3 Gene, Full-length cDNA in Lentivector, Endotoxin-free DNA

Description

TLR5 is a single-pass type I transmembrane glycoprotein belonging to the Toll-like receptor (TLR) family. The TLR family members play a fundamental role in pathogen recognition and activation of innate immunity. They serve as pattern recognition receptors for microbial pathogens. There are at least 11 mouse and 10 human TLR members. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. TLRs contain a large number of leucine­rich repeats (LRRs) and a cytoplasmic tail with a Toll/IL­1 receptor (TIR) domain. TLRs recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. TLR5 contains 22 LRR repeats and 1 LRRCT in the extracellular region. The various TLRs exhibit different patterns of expression. TLR5 recognizes bacterial flagellin, the principal component of bacterial flagella and a virulence factor. The activation of TLR5 mobilizes the nuclear factor NF-kappaB, which in turn activates a host of inflammatory-related target genes. Mutations in the TLR5 gene have been associated with both resistance and susceptibility to systemic lupus erythematosus (SLE), and susceptibility to Legionnaire disease.

 

 

Gene Symbol: TLR5; SLE1; TIL3; SLEB1; MELIOS

 

NCBI Gene ID: 7100

 

Uniprot Entry: O60602

 

Construct Details: Full length human TLR5 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter +/- an antibiotic selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles.

 

Vector Type: pLTC or pLTC-Marker (lentiviral expression vector containing a heterologous CMV promoter +/- selection marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Sequence:  

ATGGGAGACCACCTGGACCTTCTCCTAGGAGTGGTGCTCATGGCCGGTCCTGTGTTTGGAATTCCTTCCT

GCTCCTTTGATGGCCGAATAGCCTTTTATCGTTTCTGCAACCTCACCCAGGTCCCCCAGGTCCTCAACAC

CACTGAGAGGCTCCTGCTGAGCTTCAACTATATCAGGACAGTCACTGCTTCATCCTTCCCCTTTCTGGAA

CAGCTGCAGCTGCTGGAGCTCGGGAGCCAGTATACCCCCTTGACTATTGACAAGGAGGCCTTCAGAAACC

TGCCCAACCTTAGAATCTTGGACCTGGGAAGTAGTAAGATATACTTCTTGCATCCAGATGCTTTTCAGGG

ACTGTTCCATCTGTTTGAACTTAGACTGTATTTCTGTGGTCTCTCTGATGCTGTATTGAAAGATGGTTAT

TTCAGAAATTTAAAGGCTTTAACTCGCTTGGATCTATCCAAAAATCAGATTCGTAGCCTTTACCTTCATC

CTTCATTTGGGAAGTTGAATTCCTTAAAGTCCATAGATTTTTCCTCCAACCAAATATTCCTTGTATGTGA

ACATGAGCTCGAGCCCCTACAAGGGAAAACGCTCTCCTTTTTTAGCCTCGCAGCTAATAGCTTGTATAGC

AGAGTCTCAGTGGACTGGGGAAAATGTATGAACCCATTCAGAAACATGGTGCTGGAGATACTAGATGTTT

CTGGAAATGGCTGGACAGTGGACATCACAGGAAACTTTAGCAATGCCATCAGCAAAAGCCAGGCCTTCTC

TTTGATTCTTGCCCACCACATCATGGGTGCCGGGTTTGGCTTCCATAACATCAAAGATCCTGACCAGAAC

ACATTTGCTGGCCTGGCCAGAAGTTCAGTGAGACACCTGGATCTTTCACATGGGTTTGTCTTCTCCCTGA

ACTCACGAGTCTTTGAGACACTCAAGGATTTGAAGGTTCTGAACCTTGCCTACAACAAGATAAATAAGAT

TGCAGATGAAGCATTTTACGGACTTGACAACCTCCAAGTTCTCAATTTGTCATATAACCTTCTGGGGGAA

CTTTACAGTTCGAATTTCTATGGACTACCTAAGGTAGCCTACATTGATTTGCAAAAGAATCACATTGCAA

TAATTCAAGACCAAACATTCAAATTCCTGGAAAAATTACAGACCTTGGATCTCCGAGACAATGCTCTTAC

AACCATTCATTTTATTCCAAGCATACCCGATATCTTCTTGAGTGGCAATAAACTAGTGACTTTGCCAAAG

ATCAACCTTACAGCGAACCTCATCCACTTATCAGAAAACAGGCTAGAAAATCTAGATATTCTCTACTTTC

TCCTACGGGTACCTCATCTCCAGATTCTCATTTTAAATCAAAATCGCTTCTCCTCCTGTAGTGGAGATCA

AACCCCTTCAGAGAATCCCAGCTTAGAACAGCTTTTCCTTGGAGAAAATATGTTGCAACTTGCCTGGGAA

ACTGAGCTCTGTTGGGATGTTTTTGAGGGACTTTCTCATCTTCAAGTTCTGTATTTGAATCATAACTATC

TTAATTCCCTTCCACCAGGAGTATTTAGCCATCTGACTGCATTAAGGGGACTAAGCCTCAACTCCAACAG

GCTGACAGTTCTTTCTCACAATGATTTACCTGCTAATTTAGAGATCCTGGACATATCCAGGAACCAGCTC

CTAGCTCCTAATCCTGATGTATTTGTATCACTTAGTGTCTTGGATATAACTCATAACAAGTTCATTTGTG

AATGTGAACTTAGCACTTTTATCAATTGGCTTAATCACACCAATGTCACTATAGCTGGGCCTCCTGCAGA

CATATATTGTGTGTACCCTGACTCGTTCTCTGGGGTTTCCCTCTTCTCTCTTTCCACGGAAGGTTGTGAT

GAAGAGGAAGTCTTAAAGTCCCTAAAGTTCTCCCTTTTCATTGTATGCACTGTCACTCTGACTCTGTTCC

TCATGACCATCCTCACAGTCACAAAGTTCCGGGGCTTCTGTTTTATCTGTTATAAGACAGCCCAGAGACT

GGTGTTCAAGGACCATCCCCAGGGCACAGAACCTGATATGTACAAATATGATGCCTATTTGTGCTTCAGC

AGCAAAGACTTCACATGGGTGCAGAATGCTTTGCTCAAACACCTGGACACTCAATACAGTGACCAAAACA

GATTCAACCTGTGCTTTGAAGAAAGAGACTTTGTCCCAGGAGAAAACCGCATTGCCAATATCCAGGATGC

CATCTGGAACAGTAGAAAGATCGTTTGTCTTGTGAGCAGACACTTCCTTAGAGATGGCTGGTGCCTTGAA

GCCTTCAGTTATGCCCAGGGCAGGTGCTTATCTGACCTTAACAGTGCTCTCATCATGGTGGTGGTTGGGT

CCTTGTCCCAGTACCAGTTGATGAAACATCAATCCATCAGAGGCTTTGTACAGAAACAGCAGTATTTGAG

GTGGCCTGAGGATCTCCAGGATGTTGGCTGGTTTCTTCATAAACTCTCTCAACAGATACTAAAGAAAGAA

AAAGAAAAGAAGAAAGACAATAACATTCCGTTGCAAACTGTAGCAACCATCTCCTAA

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.