Human BTLA/CD272 Full-length Gene in Lentivector, Endotoxin-free DNA

Description

CD272, also known as BTLA (B- and T-lymphocyte attenuator), is a single pass type I transmembrane glycoprotein that belongs to the CD28/CTLA4 family of the Ig superfamily. Like CD28 and CTLA4, CD272 contains one Ig-like domain in the extracellular region. However, the Ig-like domain is an I­type rather than a V­type domain commonly found in other family members. CD272 also differs from other CD28/CTLA4 family members through the interaction of its Ig-like domain with the TNF superfamily member, HVEM (TNFRSF14) rather than with B7 family ligands. CD272 is an immune co-inhibitory molecule expressed on T cells, B cells and, macrophages, dendritic and NK cells.  The HVEM-CD272 ligation relays inhibitory signals to suppress the immune response. Expression of CD272 is low in naïve T cells and increased during antigen­specific induction of anergy. In B cells, BTLA is highest when cells are mature and naïve. BTLA appears to limit T cell numbers, since deletion of BTLA results in overproduction of T cells, especially CD8+ memory T cells in mice.  There are two ITIM motifs and three Tyr phosphorylation sites in the cytoplasmic tail of CD272  that mediate inhibitory signaling.   Polymorphisms in the CD272 gene have been associated with an increased risk of rheumatoid arthritis. 

 

 

Gene Symbol: CD272; BTLA; BTLA1; BTLA-1

 

NCBI Gene ID: 151888

 

Uniprot Entry: Q7Z6A9

 

Construct Details: Full length human CD272 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles.

 

Vector Type: pLTC (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 870 (bp)

 

Gene Insert Sequence:  

ATGAAGACATTGCCTGCCATGCTTGGAACTGGGAAATTATTTTGGGTCTTCTTCTTAATCCCATATCTGGACATCTGGAA

CATCCATGGGAAAGAATCATGTGATGTACAGCTTTATATAAAGAGACAATCTGAACACTCCATCTTAGCAGGAGATCCCT

TTGAACTAGAATGCCCTGTGAAATACTGTGCTAACAGGCCTCATGTGACTTGGTGCAAGCTCAATGGAACAACATGTGTA

AAACTTGAAGATAGACAAACAAGTTGGAAGGAAGAGAAGAACATTTCATTTTTCATTCTACATTTTGAACCAGTGCTTCC

TAATGACAATGGGTCATACCGCTGTTCTGCAAATTTTCAGTCTAATCTCATTGAAAGCCACTCAACAACTCTTTATGTGA

CAGATGTAAAAAGTGCCTCAGAACGACCCTCCAAGGACGAAATGGCAAGCAGACCCTGGCTCCTGTATCGTTTACTTCCT

TTGGGGGGATTGCCTCTACTCATCACTACCTGTTTCTGCCTGTTCTGCTGCCTGAGAAGGCACCAAGGAAAGCAAAATGA

ACTCTCTGACACAGCAGGAAGGGAAATTAACCTGGTTGATGCTCACCTTAAGAGTGAGCAAACAGAAGCAAGCACCAGGC

AAAATTCCCAAGTACTGCTATCAGAAACTGGAATTTATGATAATGACCCTGACCTTTGTTTCAGGATGCAGGAAGGGTCT

GAAGTTTATTCTAATCCATGCCTGGAAGAAAACAAACCAGGCATTGTTTATGCTTCCCTGAACCATTCTGTCATTGGACC

GAACTCAAGACTGGCAAGAAATGTAAAAGAAGCACCAACAGAATATGCATCCATATGTGTGAGGAGTTAA

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CGGGAAGCAATAGCATGATA-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

1. Nat. Immunol. 4:670 (2003). 

2. J. Biol. Chem. 280:39553 (2005). 

3. Nat. Immunol. 6:90 (2005). 

4. J. Immunol. 174:3377 (2005). 

5. Nat. Rev. Immunol. 6:671 (2006).

 

 

 

 

 

Product datasheet (pdf) can be downloaded here: LTP0704-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.