Human LIF Full-length Gene in Lentivector, Endotoxin-free DNA

Product ID: pLTC-hLIF (SKU#: LTP1628)

Price:

$696.00

Description

Leukemia inhibitory factor (LIF) is a member of a cytokine family that includes oncostatin M (OSM), granulocyte colony-stimulating factor (G-CSF), and interleukin 6 (IL-6). It shows a wide spectrum of biologic activities, including the growth promotion and differentiation of different types of cells, bone metabolism, cachexia, neural development, embryogenesis and inflammation. LIF has the capacity to induce differentiation in leukemic cells, embryonic stem cells, hematopoietic and neuronal cells. LIF is also a proinflammatory cytokine and induces the acute phase protein synthesis and affects the cell recruitment into the area of damage or inflammation. LIF has been implicate as an important factor for the development of the early embryo. LIF may also play a role in immune tolerance at the maternal-fetal interface. LIF supports the maintenance of mouse embryonic stem (ES) cell pluripotency and germline competency. LIF binds to the LIF receptor (LIFRα), which forms a heterodimer with a common β-subunit, GP130. This leads to activation and transduction of the JAK/STAT and MAPK signaling cascades. LIF is known as a direct transcriptional target of STAT5: STAT5 binds to LIF promoter and LIF expression is up-regulated after activation of the JAK2/STAT5 pathway. The LIF expression is significantly increased in patients with chronic myeloproliferative neoplasms. Owning to its pleiotropic functions, LIF has been involved in the pathogenesis of many diseases of various origins. Mouse LIF shows 80% sequence identity to human LIF.

Gene Symbol: LIF; CDF; DIA; HILDA; MLPLI

NCBI Gene ID: 3976

Uniprot Entry: P15018

Construct Details: Full length human LIF gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter, which can be used for both transient and stable expression in mammalian cells. It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles.

Vector Type: pLTC (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

Gene Insert Size: 609 (bp)

Gene Insert Sequence:

ATGAAGGTCTTGGCGGCAGGAGTTGTGCCCCTGCTGTTGGTTCTGCACTGGAAACATGGGGCGGGGAGCCCCCTCCCCAT

CACCCCTGTCAACGCCACCTGTGCCATACGCCACCCATGTCACAACAACCTCATGAACCAGATCAGGAGCCAACTGGCAC

AGCTCAATGGCAGTGCCAATGCCCTCTTTATTCTCTATTACACAGCCCAGGGGGAGCCGTTCCCCAACAACCTGGACAAG

CTATGTGGCCCCAACGTGACGGACTTCCCGCCCTTCCACGCCAACGGCACGGAGAAGGCCAAGCTGGTGGAGCTGTACCG

CATAGTCGTGTACCTTGGCACCTCCCTGGGCAACATCACCCGGGACCAGAAGATCCTCAACCCCAGTGCCCTCAGCCTCC

ACAGCAAGCTCAACGCCACCGCCGACATCCTGCGAGGCCTCCTTAGCAACGTGCTGTGCCGCCTGTGCAGCAAGTACCAC

GTGGGCCATGTGGACGTGACCTACGGCCCTGACACCTCGGGTAAGGATGTCTTCCAGAAGAAGAAGCTGGGCTGTCAACT

CCTGGGGAAGTATAAGCAGATCATCGCCGTGTTGGCCCAGGCCTTCTAG

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CGGGAAGCAATAGCATGATA-3’

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification. High fidelity PCR systems should always be used.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

1. EMBO J. 6:3995-4002 (1987)

2. Nature 336:690-692 (1988)

3. Proc. Natl. Acad. Sci. 85:2623-2627 (1988)

4. Cell 77:1101-1116 (1994)

5. Nat. Biotechnol. 22:707-716 (2004)

Product datasheet (pdf) can be downloaded here: LTP1628-PDS.pdf