Human BAI1/ADGRB1/GDAIF Gene, Full-length cDNA Clone in Lentivector, Endotoxin-free DNA
AGRB1 or BAI1 is a 7 transmembrane receptor belonging to the secretin receptor family, which is also known as family B or 2 of the G-protein-coupled receptors (GPCRs). Many members of this family are receptors for peptide hormones from the glucagon hormone family and regulate the activation of two major signal transduction pathways: adenylyl cyclase (cAMP) and phosphatidylinositol-calcium pathways. BAI1 is an orphan receptor involved in cell adhesion and probably in cell-cell interactions. It is a brain-specific angiogenesis inhibitor (also known as BAI1). BAI1 contains at least one 'functional' p53-binding site within an intron, and its expression has been shown to be induced by wildtype p53. There are two other brain-specific angiogenesis inhibitor genes, designated BAI2 and BAI3 which along with BAI1 have similar tissue specificities and structures. BAI1 is postulated to be an inhibitor of angiogenesis and a growth suppressor of glioblastomas.
Gene Symbol: BAI1; ADGRB1; GDAIF
NCBI Gene ID: 575
Uniprot Entry: O14514
Construct Details: Full length human BAI1 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells. It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.
Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)
Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)
Gene Insert Size: 4755 (bp)
Gene Insert Sequence:
ATGAGGGGCCAGGCCGCCGCCCCGGGCCCCGTCTGGATCCTCGCCCCGCTGCTACTGCTGCTGCTGCTGC
TGGGACGCCGCGCGCGGGCGGCCGCCGGAGCAGACGCGGGGCCCGGGCCCGAGCCGTGCGCCACGCTGGT
GCAGGGAAAGTTCTTCGGCTACTTCTCCGCGGCCGCCGTGTTCCCGGCCAACGCCTCGCGCTGCTCCTGG
ACGCTACGCAACCCGGACCCGCGGCGCTACACTCTCTACATGAAGGTGGCCAAGGCGCCCGTGCCCTGCA
GCGGCCCCGGCCGCGTGCGCACCTACCAGTTCGACTCCTTCCTCGAGTCCACGCGCACCTACCTGGGCGT
GGAGAGCTTCGACGAGGTGCTGCGGCTCTGCGACCCCTCCGCACCCCTGGCCTTCCTGCAGGCCAGCAAG
CAGTTCCTGCAGATGCGGCGCCAGCAGCCGCCCCAGCACGACGGGCTCCGGCCCCGGGCCGGGCCGCCGG
GCCCCACCGACGACTTCTCCGTGGAGTACCTGGTGGTGGGGAACCGCAACCCCAGCCGTGCCGCCTGCCA
GATGCTGTGCCGCTGGCTGGACGCGTGTCTGGCCGGTAGTCGCAGCTCGCACCCCTGCGGGATCATGCAG
ACCCCCTGCGCCTGCCTGGGCGGCGAGGCGGGCGGCCCTGCCGCGGGACCCCTGGCCCCCCGCGGGGATG
TCTGCTTGAGAGATGCGGTGGCTGGTGGCCCTGAAAACTGCCTCACCAGCCTGACCCAGGACCGGGGCGG
GCACGGCGCCACAGGCGGCTGGAAGCTGTGGTCCCTGTGGGGCGAATGCACGCGGGACTGCGGGGGAGGC
CTCCAGACGCGGACGCGCACCTGCCTGCCCGCGCCGGGCGTGGAGGGCGGCGGCTGCGAGGGGGTGCTGG
AGGAGGGTCGCCAGTGCAACCGCGAGGCCTGCGGCCCCGCTGGGCGCACCAGCTCCCGGAGCCAGTCCCT
GCGGTCCACAGATGCCCGGCGGCGCGAGGAGCTGGGGGACGAGCTGCAGCAGTTTGGGTTCCCAGCCCCC
CAGACCGGTGACCCAGCAGCCGAGGAGTGGTCCCCGTGGAGCGTGTGCTCCAGCACCTGCGGCGAGGGCT
GGCAGACCCGCACGCGCTTCTGCGTGTCCTCCTCCTACAGCACGCAGTGCAGCGGACCCCTGCGCGAGCA
GCGGCTGTGCAACAACTCTGCCGTGTGCCCAGTGCATGGTGCCTGGGATGAGTGGTCGCCCTGGAGCCTC
TGCTCCAGCACCTGTGGCCGTGGCTTTCGGGATCGCACGCGCACCTGCAGGCCCCCCCAGTTTGGGGGCA
ACCCCTGTGAGGGCCCTGAGAAGCAAACCAAGTTCTGCAACATTGCCCTGTGCCCTGGCCGGGCAGTGGA
TGGAAACTGGAATGAGTGGTCGAGCTGGAGCGCCTGCTCCGCCAGCTGCTCCCAGGGCCGACAGCAGCGC
ACGCGTGAATGCAACGGGCCTTCCTACGGGGGTGCGGAGTGCCAGGGCCACTGGGTGGAGACCCGAGACT
GCTTCCTGCAGCAGTGCCCAGTGGATGGCAAGTGGCAGGCCTGGGCGTCATGGGGCAGTTGCAGCGTCAC
GTGTGGGGCTGGCAGCCAGCGACGGGAGCGTGTCTGCTCTGGGCCCTTCTTCGGGGGAGCAGCCTGCCAG
GGCCCCCAGGATGAGTACCGGCAGTGCGGCACCCAGCGGTGTCCCGAGCCCCATGAGATCTGTGATGAGG
ACAACTTTGGTGCTGTGATCTGGAAGGAGACCCCAGCGGGAGAGGTGGCTGCTGTCCGGTGTCCCCGCAA
CGCCACAGGACTCATCCTGCGACGGTGTGAGCTGGACGAGGAAGGCATCGCCTACTGGGAGCCCCCCACC
TACATCCGCTGTGTTTCCATTGACTACAGAAACATCCAGATGATGACCCGGGAGCACCTGGCCAAGGCTC
AGCGAGGGCTGCCTGGGGAGGGGGTCTCGGAGGTCATCCAGACACTGGTGGAGATCTCTCAGGACGGGAC
CAGCTACAGTGGGGACCTGCTGTCCACCATCGATGTCCTGAGGAACATGACAGAGATTTTCCGGAGAGCG
TACTACAGCCCCACCCCTGGGGACGTACAGAACTTTGTCCAGATCCTTAGCAACCTGTTGGCAGAGGAGA
ATCGGGACAAGTGGGAGGAGGCCCAGCTGGCGGGCCCCAACGCCAAGGAGCTGTTCCGGCTGGTGGAGGA
CTTTGTGGACGTCATCGGCTTCCGCATGAAGGACCTGAGGGATGCATACCAGGTGACAGACAACCTGGTT
CTCAGCATCCATAAGCTCCCAGCCAGCGGAGCCACTGACATCAGCTTCCCCATGAAGGGCTGGCGGGCCA
CGGGTGACTGGGCCAAGGTGCCAGAGGACAGGGTCACTGTGTCCAAGAGTGTCTTCTCCACGGGGCTGAC
AGAGGCCGATGAAGCATCCGTGTTTGTGGTGGGCACCGTGCTCTACAGGAACCTGGGCAGCTTCCTGGCC
CTGCAGAGGAACACGACCGTCCTGAATTCTAAGGTGATCTCCGTGACTGTGAAACCCCCGCCTCGCTCCC
TGCGCACACCCTTGGAGATCGAGTTTGCCCACATGTATAATGGCACCACCAACCAGACCTGTATCCTGTG
GGATGAGACGGATGTACCCTCCTCCTCCGCCCCCCCGCAGCTCGGGCCCTGGTCGTGGCGCGGCTGCCGC
ACGGTGCCCCTCGACGCCCTCCGGACGCGCTGCCTCTGTGACCGGCTCTCCACCTTCGCCATCTTAGCCC
AGCTCAGCGCCGACGCGAACATGGAGAAGGCGACTCTGCCGTCGGTGACGCTCATCGTGGGCTGTGGCGT
GTCCTCTCTCACCCTGCTCATGCTGGTCATCATCTACGTGTCCGTGTGGAGGTACATTCGCTCAGAGCGT
TCTGTCATCCTCATCAACTTCTGCCTGTCCATCATCTCCTCCAATGCCCTCATCCTCATCGGGCAGACCC
AGACCCGCAACAAGGTGGTGTGCACGCTGGTGGCCGCCTTCCTGCACTTCTTCTTCCTGTCCTCCTTCTG
CTGGGTGCTCACCGAGGCCTGGCAGTCCTACATGGCGGTGACGGGCCACCTCCGGAACCGCCTCATCCGC
AAGCGCTTCCTCTGCCTGGGCTGGGGGCTCCCTGCACTGGTTGTGGCCATTTCTGTGGGATTCACCAAGG
CCAAAGGGTACAGCACCATGAACTACTGCTGGCTCTCCCTGGAGGGGGGACTGCTCTATGCCTTCGTGGG
ACCTGCCGCTGCCGTTGTGCTGGTGAACATGGTCATTGGGATCCTGGTGTTCAACAAGCTCGTGTCCAAA
GACGGCATCACGGACAAGAAGCTGAAGGAGCGGGCAGGGGCCTCCCTGTGGAGCTCCTGCGTGGTGCTGC
CGCTGCTGGCGCTGACCTGGATGTCGGCTGTGCTCGCCGTCACCGACCGCCGCTCCGCCCTCTTCCAGAT
CCTCTTCGCTGTCTTCGACTCGCTGGAGGGCTTCGTCATCGTCATGGTGCACTGTATCCTCCGTAGAGAG
GTCCAGGACGCTGTGAAATGCCGTGTGGTTGACCGGCAGGAGGAGGGCAACGGGGACTCAGGGGGCTCCT
TCCAGAACGGCCACGCCCAGCTCATGACCGACTTCGAGAAGGACGTGGATCTGGCCTGTAGATCAGTGCT
GAACAAGGACATCGCGGCCTGCCGCACTGCCACCATCACGGGCACACTGAAGCGGCCGTCTCTGCCCGAG
GAGGAGAAGCTGAAGCTGGCCCATGCCAAGGGGCCGCCCACCAATTTCAACAGCCTGCCGGCCAACGTGT
CCAAGCTGCACCTGCACGGCTCACCCCGCTATCCCGGCGGGCCCCTGCCCGACTTCCCCAACCACTCACT
GACCCTCAAGAGGGACAAGGCGCCCAAGTCCTCCTTCGTCGGTGACGGGGACATCTTCAAGAAGCTGGAC
TCGGAGCTGAGCCGGGCCCAGGAGAAGGCTCTGGACACGAGCTACGTGATCCTGCCCACGGCCACGGCCA
CGCTGCGGCCCAAGCCCAAGGAGGAGCCCAAGTACAGCATCCACATTGACCAGATGCCGCAGACCCGCCT
CATCCACCTCAGCACGGCCCCCGAGGCCAGCCTCCCCGCCCGCAGCCCGCCCTCCCGCCAGCCCCCCAGC
GGCGGGCCCCCCGAGGCACCCCCTGCCCAGCCCCCACCGCCTCCGCCCCCACCGCCACCACCTCCCCAGC
AGCCCCTGCCCCCACCGCCCAATCTGGAGCCGGCACCCCCCAGCCTGGGGGATCCCGGGGAGCCTGCCGC
CCATCCGGGACCCAGCACGGGGCCCAGCACCAAGAACGAGAATGTCGCCACCTTGTCTGTGAGCTCCCTG
GAGCGGCGGAAGTCGCGGTATGCAGAACTGGACTTTGAGAAGATCATGCACACCCGGAAGCGGCACCAAG
ACATGTTCCAGGACCTGAACCGGAAGCTGCAGCACGCAGCGGAGAAGGACAAGGAGGTGCTGGGGCCGGA
CAGCAAGCCGGAAAAGCAGCAGACGCCCAACAAGAGGCCCTGGGAGAGCCTCCGGAAAGCCCACGGGACG
CCCACGTGGGTGAAGAAGGAGCTGGAGCCGCTGCAGCCGTCGCCGCTGGAGCTTCGCAGCGTGGAGTGGG
AGAGGTCGGGCGCCACGATCCCGCTGGTGGGCCAGGACATCATCGACCTCCAGACCGAGGTCTGA
Primers: Gene insert coding sequence can be confirmed by following primers:
(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;
(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’
Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification. High fidelity PCR systems should always be used.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.
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Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.
1. Ann Rev Biochem 56: 615 (1987)
2. Genome Biol. 2: S3013 (2001)
3. Science 301: 361 (2003)
4. Physiol Rev 85: 1159 (2005)
5. Genomics 88: 263 (2006)
Additional supporting documents, including product datasheet, COA and MSDS are available upon request.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.