Human Nanog Full-length Gene in Lentivector, Endotoxin-free DNA

Product ID: pLTE-Nanog (SKU#: LTE0023)

Price:

$696.00

Description

Nanog is a transcription factor involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. It contains 1 conserved homeobox DNA-binding domain at the N-terminal region and is the founding member of the Nanog homeobox family. Nanog binds optimally to the DNA consensus sequence 5'-TAAT[G/T][G/T]-3' or 5'-[C/G][G/A][C/G]C[G/C]ATTAN[G/C]-3'. Nanog is expressed in embryonic stem (ES) and carcinoma (EC) cells. Nanog may be useful in the immunohistochemical diagnosis of tumors. NANOG is thought to be a key factor in maintaining pluripotency and function in concert with other transcription factors such as Oct-4 (POU5F1) and SOX2 to establish ES cell identity bearing the ability to become virtually any cell of any of the 3 germ layers (endoderm, ectoderm, mesoderm). Overexpression of Nanog in mouse embryonic stem cells causes them to self-renew in the absence of Leukemia inhibitory factor (LIF). Overexpression of Nanog in human embryonic stem cells enables their propagation for multiple passages during which the cells remain pluripotent. Loss of Nanog function causes differentiation of mouse or human embryonic stem cells into other cell types.

Gene Symbol: Nanog

NCBI Gene ID: 79923

Uniprot Entry: Q9H950

Construct Details: Full length human Nanog gene is subcloned into the Lentiviral expression vector pLTE with an upstream EF1α promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells. It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple selection markers are available.

Vector Type: pLTE or pLTE-IRES-Marker (lentiviral expression vector containing a heterologous EF1α promoter, see the vector map above)

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

Gene Insert Size: 918 (bp)

Gene Insert Sequence:

ATGAGTGTGGATCCAGCTTGTCCCCAAAGCTTGCCTTGCTTTGAAGCATCCGACTGTAAAGAATCTTCACCTATGCCTGT

GATTTGTGGGCCTGAAGAAAACTATCCATCCTTGCAAATGTCTTCTGCTGAGATGCCTCACACGGAGACTGTCTCTCCTC

TTCCTTCCTCCATGGATCTGCTTATTCAGGACAGCCCTGATTCTTCCACCAGTCCCAAAGGCAAACAACCCACTTCTGCA

GAGAAGAGTGTCGCAAAAAAGGAAGACAAGGTCCCGGTCAAGAAACAGAAGACCAGAACTGTGTTCTCTTCCACCCAGCT

GTGTGTACTCAATGATAGATTTCAGAGACAGAAATACCTCAGCCTCCAGCAGATGCAAGAACTCTCCAACATCCTGAACC

TCAGCTACAAACAGGTGAAGACCTGGTTCCAGAACCAGAGAATGAAATCTAAGAGGTGGCAGAAAAACAACTGGCCGAAG

AATAGCAATGGTGTGACGCAGAAGGCCTCAGCACCTACCTACCCCAGCCTTTACTCTTCCTACCACCAGGGATGCCTGGT

GAACCCGACTGGGAACCTTCCAATGTGGAGCAACCAGACCTGGAACAATTCAACCTGGAGCAACCAGACCCAGAACATCC

AGTCCTGGAGCAACCACTCCTGGAACACTCAGACCTGGTGCACCCAATCCTGGAACAATCAGGCCTGGAACAGTCCCTTC

TATAACTGTGGAGAGGAATCTCTGCAGTCCTGCATGCAGTTCCAGCCAAATTCTCCTGCCAGTGACTTGGAGGCTGCCTT

GGAAGCTGCTGGGGAAGGCCTTAATGTAATACAGCAGACCACTAGGTATTTTAGTACTCCACAAACCATGGATTTATTCC

TAAACTACTCCATGAACATGCAACCTGAAGACGTGTGA

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAGCCTCAGACAGTGGTTC-3’

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification. High fidelity PCR systems should always be used.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

1. Cell 113:631 (2003)

2. Stem Cells 22:169 (2004)

3. Cell 113:643 (2003)

4. Histopathology 47:48 (2005)

5. Stem Cells 23:1035 (2005)

Product datasheet (pdf) can be downloaded here: LTE0023-PDS.pdf