Human CD5/Leu1/Ly1/T1 Gene, Full-length cDNA Clone in Lentivector, Endotoxin-free DNA

Description

T-cell surface glycoprotein CD5, also known as Leu­1, Ly­1, and T1, is a single pass type I transmembrane protein belonging to the scavenger receptor superfamily. CD5 contains a 348 amino acid (aa) extracellular domain (ECD) with 3 scavenger receptor cysteine­rich (SRCR) domains. Crystal structure of the 3rd ECD of CD5 reveals the fold of a group B SRCR domain. The ECD of CD5 can be cleaved and circulates in the serum. T cell antigen receptor (TCR) positively regulates CD5 expression on developing thymocytes and on peripheral CD4+ T cells.  CD5 is known to inhibit TCR signaling and promotes T cell anergy and survival. CD5 signaling negatively regulates the generation of regulatory T cells (Treg) but promotes the development of Th17 cells. CD5 interacts with CD72/Lyb-2 on B cells and with PTPN6/SHP-1 for intracellular signaling. Within the B cell lineage, CD5 is expressed on B­1a cells, anergic B cells, and IL­10 producing regulatory B cells. Similar to its role on T cells, CD5 negatively regulates signaling through the B cell antigen receptor (BCR) and supports B cell survival, anergy, and tolerance. CD5 is also involved in the cellular entry of hepatitis C virus into T lymphocytes.

 

 

Gene Symbol: CD5; Leu-1; Ly-1; T1; LEU1; Ly1

 

NCBI Gene ID: 921

 

Uniprot Entry: P06127

 

Construct Details: Full length human CD5 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1488 (bp)

 

Gene Insert Sequence:  

ATGCCCATGGGGTCTCTGCAACCGCTGGCCACCTTGTACCTGCTGGGGATGCTGGTCGCTTCCTGCCTCGGACGGCTCAG

CTGGTATGACCCAGATTTCCAGGCAAGGCTCACCCGTTCCAACTCGAAGTGCCAGGGCCAGCTGGAGGTCTACCTCAAGG

ACGGATGGCACATGGTTTGCAGCCAGAGCTGGGGCCGGAGCTCCAAGCAGTGGGAGGACCCCAGTCAAGCGTCAAAAGTC

TGCCAGCGGCTGAACTGTGGGGTGCCCTTAAGCCTTGGCCCCTTCCTTGTCACCTACACACCTCAGAGCTCAATCATCTG

CTACGGACAACTGGGCTCCTTCTCCAACTGCAGCCACAGCAGAAATGACATGTGTCACTCTCTGGGCCTGACCTGCTTAG

AACCCCAGAAGACAACACCTCCAACGACAAGGCCCCCGCCCACCACAACTCCAGAGCCCACAGCTCCTCCCAGGCTGCAG

CTGGTGGCACAGTCTGGCGGCCAGCACTGTGCCGGCGTGGTGGAGTTCTACAGCGGCAGCCTGGGGGGTACCATCAGCTA

TGAGGCCCAGGACAAGACCCAGGACCTGGAGAACTTCCTCTGCAACAACCTCCAGTGTGGCTCCTTCTTGAAGCATCTGC

CAGAGACTGAGGCAGGCAGAGCCCAAGACCCAGGGGAGCCACGGGAACACCAGCCCTTGCCAATCCAATGGAAGATCCAG

AACTCAAGCTGTACCTCCCTGGAGCATTGCTTCAGGAAAATCAAGCCCCAGAAAAGTGGCCGAGTTCTTGCCCTCCTTTG

CTCAGGTTTCCAGCCCAAGGTGCAGAGCCGTCTGGTGGGGGGCAGCAGCATCTGTGAAGGCACCGTGGAGGTGCGCCAGG

GGGCTCAGTGGGCAGCCCTGTGTGACAGCTCTTCAGCCAGGAGCTCGCTGCGGTGGGAGGAGGTGTGCCGGGAGCAGCAG

TGTGGCAGCGTCAACTCCTATCGAGTGCTGGACGCTGGTGACCCAACATCCCGGGGGCTCTTCTGTCCCCATCAGAAGCT

GTCCCAGTGCCACGAACTTTGGGAGAGAAATTCCTACTGCAAGAAGGTGTTTGTCACATGCCAGGATCCAAACCCCGCAG

GCCTGGCCGCAGGCACGGTGGCAAGCATCATCCTGGCCCTGGTGCTCCTGGTGGTGCTGCTGGTCGTGTGCGGCCCCCTT

GCCTACAAGAAGCTAGTGAAGAAATTCCGCCAGAAGAAGCAGCGCCAGTGGATTGGCCCAACGGGAATGAACCAAAACAT

GTCTTTCCATCGCAACCACACGGCAACCGTCCGATCCCATGCTGAGAACCCCACAGCCTCCCACGTGGATAACGAATACA

GCCAACCTCCCAGGAACTCCCACCTGTCAGCTTATCCAGCTCTGGAAGGGGCTCTGCATCGCTCCTCCATGCAGCCTGAC

AACTCCTCCGACAGTGACTATGATCTGCATGGGGCTCAGAGGCTGTAA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

1. Nature 323:346 (1986)

2. Nature 351:662 (1991) 

3. Science 269:535 (1995)

4. J. Exp. Med. 191:883 (2000)

5. Immunity 20:695 (2004)

6. J. Biol. Chem. 282:12669 (2007)

 

 

 

 

Product datasheet (pdf) can be downloaded here: LTP2305-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.