Human TGFB1/TGFβ1-LAP Protein Complex, Biotinylated, Fc-fusion, Recombinant

Product ID: TGFB1-Fc-Biot (SKU#: FCL2093B)

Price:

$658.00

Description

The TGFβ (transforming growth factor β) superfamily proteins are pleiotropic cytokines that regulate a diverse range of cellular processes, including proliferation, differentiation, migration, adhesion and apoptosis. The TGFβ superfamily elicits 4 signaling pathways: TGFβ, Bone Morphogenic Protein (BMP), Activin, and Nodal. Each pathway signals through a heteromeric receptor complex composed of at least 2 type-I and 2 type-II receptors with cytoplasmic serine/threonine kinase domains. The receptor complex phosphorylates and activates Smad proteins to regulate downstream gene transcription. There are 3 closely related mammalian members (TGFβ1-3) of the TGFβ family that share a characteristic cystine knot structure. They are known to function as cellular switches that regulate immune function, proliferation and epithelialmesenchymal transition (EMT). Each TGFβ isoform has non-redundant functions. For instance, TGFβ1-deficient mice display defects in hematopoiesis and endothelial differentiation, and die of overwhelming inflammation. TGFβ1 is highly expressed in bone and acts as a stimulator of bone formation. A furinlike convertase processes it to generate an Nterminal 249 aa latencyassociated peptide (LAP) and a Cterminal 112 aa mature TGFβ1. Mature human TGFβ1 is 100% identical to pig, dog and cow TGFβ1, and 99% identical to mouse, rat and horse TGFβ1, therefore showing crossspecies activity. TGFβs are identified as potential therapeutic targets for cancer and cardiovascular diseases. This gene is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease.

Gene Symbol: TGF-beta1; CED; LAP; DPD1; TGFB; TGFbeta

NCBI Gene ID: 7040

Uniprot Entry: P01137

Construct Details: The recombinant human TGFB1-Fc fusion is expressed as a 600 amino acid protein consisting of Leu30 - Ser390 region of TGFB1 (Uniprot Accession #P01137) and a C-terminal Fc from human IgG1, which exists as a disulfide-linked dimeric LAP-TGFβ1-Fc complex under non-reducing conditions.

Source: Human cells stably expressing TGFB1-Fc growing in chemical-defined media with no animal components or antibiotics

Deduced Amino Acid Sequence:

10 20 30 40 50 60

LSTCKTIDME LVKRKRIEAI RGQILSKLRL ASPPSQGEVP PGPLPEAVLA LYNSTRDRVA

70 80 90 100 110 120

GESAEPEPEP EADYYAKEVT RVLMVETHNE IYDKFKQSTH SIYMFFNTSE LREAVPEPVL

130 140 150 160 170 180

LSRAELRLLR LKLKVEQHVE LYQKYSNNSW RYLSNRLLAP SDSPEWLSFD VTGVVRQWLS

190 200 210 220 230 240

RGGEIEGFRL SAHCSCDSRD NTLQVDINGF TTGRRGDLAT IHGMNRPFLL LMATPLERAQ

250 260 270 280 290 300

HLQSSRHRRA LDTNYCFSST EKNCCVRQLY IDFRKDLGWK WIHEPKGYHA NFCLGPCPYI

310 320 330 340 350 360

WSLDTQYSKV LALYNQHNPG ASAAPCCVPQ ALEPLPIVYY VGRKPKVEQL SNMIVRSCKC

370 380 390 400 410 420

SGSTTENLYF QGSTGTHTCP PCPAPELLGG PSVFLFPPKP KDTLMISRTP EVTCVVVDVS

430 440 450 460 470 480

HEDPEVKFNW YVDGVEVHNA KTKPREEQYN STYRVVSVLT VLHQDWLNGK EYKCKVSNKA

490 500 510 520 530 540

LPAPIEKTIS KAKGQPREPQ VYTLPPSREE MTKNQVSLTC LVKGFYPSDI AVEWESNGQP

550 560 570 580 590 600

ENNYKTTPPV LDSDGSFFLY SKLTVDKSRW QQGNVFSCSV MHEALHNHYT QKSLSLSPGK

M.W.: Calculated molecular mass (kDa): 68; Estimated by SDS-PAGE under reducing condition (kDa): 40-45 & 70-75

Calculated PI: 8.3

Calculated Extinction Coefficient (M^-1 cm^-1, at 280nm): 94865

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as DTT "+")

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier and preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

Biological Activity: Binds to its receptor human ALK1 protein and anti-TGFB1 monoclonal antibodies, human IgG1 (SKU#MAB2005) and human IgG4 (SKU#MAB1561).

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

The product is shipped at 4°C for immediate use. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

1. J. Biol. Chem. 258: 7155 (1983)

2. Nature 316: 701 (1985)

3. Annual Rev. Immunol. 16: 137 (1998)

4. Cell 75: 671 (1993).

5. Cytokine Growth Factor Rev. 18: 279 (2007).

Product datasheet (pdf) can be downloaded here: FCL2093B-PDS.pdf