Human VNN2/Vanin-2/GPI-80 Gene, Full-length cDNA Clone in Lentivector, Endotoxin-free DNA

Description

VNN2 (vascular non-inflammatory molecule 2), also known as glycosyl- phosphatidyl inositol-anchored protein GPI-80, Vanin-2, and FOAP-4, is a GPI-anchored cell membrane protein belonging to the Vanin/BTD/VNN family of the carbon-nitrogen (CN) hydrolase family. The Vanin family includes secreted and membrane- associated proteins, a few of which have been reported to participate in hematopoietic cell trafficking. VNN2 shows high sequence similarity with other Vanin family members, and also with biotinidase. No biotinidase activity has been demonstrated for any of the vanin proteins.  VNN2 is an amidohydrolase that hydrolyzes specifically one of the carboamide linkages in D-pantetheine thus recycling pantothenic acid (vitamin B5) and releasing cysteamine. The pantetheinase activity of VNN2 may play a role in oxidative-stress response. VNN2 is widely expressed with higher expression in spleen and blood. VNN2 is involved in the thymus homing of bone marrow cells. It may regulate beta-2 integrin-mediated cell adhesion, migration and motility of neutrophil. Elevated VNN2 expression is associated with development and progression of thymoma.

 

 

Gene Symbol: VNN2; vanin 2; Vanin-2; FOAP-4; GPI-80

 

NCBI Gene ID: 8875

 

Uniprot Entry: Q95498

 

Construct Details: Full length human VNN2/Vanin-2/GPI-80 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1563 (bp)

 

Gene Insert Sequence:  

ATGGTCACTTCCTCTTTTCCAATCTCTGTGGCAGTTTTTGCCCTAATAACCCTGCAGGTTGGTACTCAGGACAGTTTTAT

AGCTGCAGTGTATGAACATGCTGTCATTTTGCCAAATAAAACAGAAACACCAGTTTCTCAGGAGGATGCCTTGAATCTCA

TGAACGAGAATATAGACATTCTGGAGACAGCGATCAAGCAGGCAGCTGAGCAGGGTGCTCGAATCATTGTGACTCCAGAA

GATGCACTTTATGGATGGAAATTTACCAGGGAAACTGTTTTCCCTTATCTGGAGGATATCCCAGACCCTCAGGTGAACTG

GATTCCGTGTCAAGACCCCCACAGATTTGGTCACACACCAGTACAAGCAAGACTCAGCTGCCTGGCCAAGGACAACTCTA

TCTATGTCTTGGCAAATTTGGGGGACAAAAAGCCATGTAATTCCCGTGACTCCACATGTCCTCCTAATGGCTACTTTCAA

TACAATACCAATGTGGTGTATAATACAGAAGGAAAACTCGTGGCACGTTACCATAAGTACCACCTGTACTCTGAGCCTCA

GTTTAATGTCCCTGAAAAGCCGGAGTTGGTGACTTTCAACACCGCATTTGGAAGGTTTGGCATTTTCACGTGCTTTGATA

TATTCTTCTATGATCCTGGTGTTACCCTGGTGAAAGATTTCCATGTGGACACCATACTGTTTCCCACAGCTTGGATGAAC

GTTTTGCCCCTTTTGACAGCTATTGAATTCCATTCAGCTTGGGCAATGGGAATGGGAGTTAATCTTCTTGTGGCCAACAC

ACATCATGTCAGCCTAAATATGACAGGAAGTGGTATTTATGCACCAAATGGTCCCAAAGTGTATCATTATGACATGAAGA

CAGAGTTGGGAAAACTTCTCCTTTCAGAGGTGGATTCACATCCCCTATCCTCGCTTGCCTACCCAACAGCTGTTAATTGG

AATGCCTACGCCACCACCATCAAACCATTTCCAGTACAGAAAAACACTTTCAGGGGATTTATTTCCAGGGATGGGTTCAA

CTTCACAGAACTTTTTGAAAATGCAGGAAACCTTACAGTCTGTCAAAAGGAGCTTTGCTGTCATTTAAGCTACAGAATGT

TACAAAAAGAAGAGAATGAAGTATACGTTCTAGGAGCTTTTACAGGATTACATGGCCGAAGGAGAAGAGAGTACTGGCAG

GTCTGCACACTGCTGAAGTGCAAAACTACTAATTTGACAACTTGTGGACGGCCAGTAGAAACTGCTTCTACAAGATTTGA

AATGTTCTCCCTCAGTGGCACATTTGGAACAGAGTATGTTTTTCCTGAAGTGCTACTTACCGAAATTCATCTGTCACCTG

GAAAATTTGAGGTGCTGAAAGATGGGCGTTTGGTAAACAAGAATGGATCATCTGGGCCTATACTAACAGTGTCACTCTTT

GGGAGGTGGTACACAAAGGACTCACTTTACAGCTCATGTGGGACCAGCAATTCAGCAATAACTTACCTGCTAATATTCAT

ATTATTAATGATCATAGCTTTGCAAAATATTGTAATGTTATAG

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

1. Genomics 53:203 (1998)

2. J. Immunol. 162:4277 (1999) 

3. Immunogenetics. 53:296 (2001)

4. Cancer Sci. 94:809 (2003)

5. J. Invest. Dermatol. 129: 2167 (2009)

 

 

 

 

Product datasheet (pdf) can be downloaded here: LTP2116-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.