Human DcR2 Full-length Gene in Lentivector, Endotoxin-free DNA

Description

DcR2 ( decoy receptor 2), also known as CD264, TRAILR4, and TRUNDD, is a Single-pass type I transmembrane protein of the TNF receptor superfamily (TNFRSF10D).  DcR2 contains an extracellular TRAIL-binding domain with 3 TNFR-cysteine repeats, a transmembrane domain, and a truncated cytoplasmic death domain. Therefore DcR2 does not induce apoptosis, and may play an inhibitory role in TRAIL-induced cell apoptosis. DcR2 is widely expressed, in particular in fetal kidney, lung and liver, and in adult testis and liver. It is also expressed in peripheral blood leukocytes, colon and small intestine, ovary, prostate, thymus, spleen, pancreas, kidney, lung, placenta and heart. Aberrant methylation of DcR2 is frequent in multiple tumor types. Overexpression of TRAIL R4 can protect cells bearing TRAILR1 and/or TRAILR2 from TRAIL­mediated apoptosis. The human soluble DcR2-Fc chimera neutralizes the ability of TRAIL to induce apoptosis.

 

 

Gene Symbol: DCR2; CD264; TRUNDD; TRAILR4; TRAIL-R4; UNQ251/PRO288

 

NCBI Gene ID: 8793

 

Uniprot Entry: Q9UBN6

 

Construct Details: Full length human DcR2/CD264 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1161 (bp)

 

Gene Insert Sequence:  

ATGGGACTTTGGGGACAAAGCGTCCCGACCGCCTCGAGCGCTCGAGCAGGGCGCTATCCAGGAGCCAGGA

CAGCGTCGGGAACCAGACCATGGCTCCTGGACCCCAAGATCCTTAAGTTCGTCGTCTTCATCGTCGCGGT

TCTGCTGCCGGTCCGGGTTGACTCTGCCACCATCCCCCGGCAGGACGAAGTTCCCCAGCAGACAGTGGCC

CCACAGCAACAGAGGCGCAGCCTCAAGGAGGAGGAGTGTCCAGCAGGATCTCATAGATCAGAATATACTG

GAGCCTGTAACCCGTGCACAGAGGGTGTGGATTACACCATTGCTTCCAACAATTTGCCTTCTTGCCTGCT

ATGTACAGTTTGTAAATCAGGTCAAACAAATAAAAGTTCCTGTACCACGACCAGAGACACCGTGTGTCAG

TGTGAAAAAGGAAGCTTCCAGGATAAAAACTCCCCTGAGATGTGCCGGACGTGTAGAACAGGGTGTCCCA

GAGGGATGGTCAAGGTCAGTAATTGTACGCCCCGGAGTGACATCAAGTGCAAAAATGAATCAGCTGCCAG

TTCCACTGGGAAAACCCCAGCAGCGGAGGAGACAGTGACCACCATCCTGGGGATGCTTGCCTCTCCCTAT

CACTACCTTATCATCATAGTGGTTTTAGTCATCATTTTAGCTGTGGTTGTGGTTGGCTTTTCATGTCGGA

AGAAATTCATTTCTTACCTCAAAGGCATCTGCTCAGGTGGTGGAGGAGGTCCCGAACGTGTGCACAGAGT

CCTTTTCCGGCGGCGTTCATGTCCTTCACGAGTTCCTGGGGCGGAGGACAATGCCCGCAACGAGACCCTG

AGTAACAGATACTTGCAGCCCACCCAGGTCTCTGAGCAGGAAATCCAAGGTCAGGAGCTGGCAGAGCTAA

CAGGTGTGACTGTAGAGTTGCCAGAGGAGCCACAGCGTCTGCTGGAACAGGCAGAAGCTGAAGGGTGTCA

GAGGAGGAGGCTGCTGGTTCCAGTGAATGACGCTGACTCCGCTGACATCAGCACCTTGCTGGATGCCTCG

GCAACACTGGAAGAAGGACATGCAAAGGAAACAATTCAGGACCAACTGGTGGGCTCCGAAAAGCTCTTTT

ATGAAGAAGATGAGGCAGGCTCTGCTACGTCCTGCCTGTGA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

1. Cur. Biol. 7:1003 (1997) 

2. Immunity 7:813 (1997)

3. Curr. Opin. Immunol. 10:559 (1998) 

4. FEBS lett 424:41 (1998) 

5. Mol Cell Biol. 26:7046 (2006)

 

 

 

 

Product datasheet (pdf) can be downloaded here: LTP0474-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.