Human RELT Protein, ECD (Extracellular Domain), Fc-fusion, Biotinylated, Recombinant

Description

RELT (receptor expressed in lymphoid tissues) is a single-pass, type I transmembrane glycoprotein in the TNF receptor superfamily (TNFRSF19L). RELT is primarily expressed in hematopoietic tissues with highest levels in spleen, lymph node, thymus, peripheral blood leukocytes, bone marrow and fetal liver. It is also found in in leukemias and lymphomas.  RELT has a 137 amino acid (aa) extracellular domain containing one TNFR cysteine­rich repeat and a 247 aa cytoplasmic region that lacks a death domain. The ECD of RELT is most closely related to that of TROY (TNFRSF19) and OX40 (TNFRSF4). RELT has been shown to selectively bind the adaptor protein TNF receptor-associated factor 1 (TRAF1).  Overexpression of RELT can activate the NF-kB, p38 and JNK signaling pathways and induces apoptosis. RELT is also capable of stimulating T-cell proliferation in the presence of CD3 signaling.  However RELT does not bind any of the TNF superfamily ligands. Two related transmembrane proteins, RELL1 and RELL2, have been identified.  Both RELL1 and RELL2 can interact with RELT and may modulate its signaling activity.

  

 

Gene Symbol: TRLT; TNFRSF19L

  

NCBI Gene ID: 84957

 

Uniprot Entry: Q969Z4

 

Construct Details: The recombinant human RELT-Fc fusion is expressed as a 365 amino acid protein consisting of Ser26 - Gln162 region of RELT (UniProt accession #Q969Z4) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions (see the gel image inserted above).  

  

Source: Human cells stably expressing human RELT-Fc and growing in chemical-defined media with no animal components or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60 

STTLWQCPPG EEPDLDPGQG TLCRPCPPGT FSAAWGSSPC QPHARCSLWR RLEAQVGMAT  

        70         80         90        100        110        120 

RDTLCGDCWP GWFGPWGVPR VPCQPCSWAP LGTHGCDEWG RRARRGVEVA AGASSGGETR  

       130        140        150        160        170        180 

QPGNGTRAGG PEETAAQSTG THTCPPCPAP ELLGGPSVFL FPPKPKDTLM ISRTPEVTCV 

       190        200        210        220        230        240 

VVDVSHEDPE VKFNWYVDGV EVHNAKTKPR EEQYNSTYRV VSVLTVLHQD WLNGKEYKCK 

       250        260        270        280        290        300 

VSNKALPAPI EKTISKAKGQ PREPQVYTLP PSREEMTKNQ VSLTCLVKGF YPSDIAVEWE  

       310        320        330        340        350        360 

SNGQPENNYK TTPPVLDSDG SFFLYSKLTV DKSRWQQGNV FSCSVMHEAL HNHYTQKSLS 

 

LSPGK 

 

M.W.: Calculated molecular mass (kDa): 40; Estimated by SDS-PAGE under reducing condition (kDa): 45-55

 

Calculated PI: 6.74

 

Calculated extinction coefficients (M-1 cm-1, at 280nm):  80410

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "DTT: +")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Not Available

 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks.

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

1. Blood. 97: 2702 (2001).

2. Proc.Natl.Acad.Sci. 99: 16899 (2003)

3. J. Biol. Chem. 281:13964(2006).

4. Biochem. Biophys. Res. Commun. 340:535 (2006).

5. Cell. Immunol. 261:1 (2010).

 

 

 

 

Product datasheet (pdf) can be downloaded here: FCL0041B-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.