Mouse PD-L2 protein, ECD (extracellular domain), Fc-fusion, recombinant

Product ID: mPD-L2-Fc (SKU#: FCL1818)

Price:

$598.00

Description

PD-L2 (programmed death ligand 2), also referred to as B7-DC or CD273, is a single pass type I transmembrane glycoprotein that belongs to the B7 family of the Ig superfamily. It was originally identified as a homolog of PD-L1. Like PD-L1 and other B7 family members, PD-L2 contains one Ig V-like and one Ig C-like domain in the extracellular region. Among the family members, they share about 20-25% amino acid identity. PDL1 and PDL2 share ~41% amino acid sequence identity and have similar functions. PD-L2 is expressed on antigen presenting cells, placental endothelium and medullary thymic epithelial cells, and can be induced by LPS and INF-γ. PD-L2 and PD-L1 are two ligands for PD-1/CD279, a member of the CD28/CTLA4 family receptors that play critical roles in regulating T cell activation and immune tolerance. Interaction with PD-1, which has a cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM), inhibits T-cell proliferation and cytokine production. PD-L2 is also a dendritic cell molecule with co-stimulatory properties for T cells. The interaction of PD-L2/PD-1 has a 2-6-fold higher affinity than that of B7-H1/PD-1.

Gene Symbol: PD-L2; CD273; B7-DC; PDL2; PDCD1LG2; B7DC; CD273; PDCD1L2; PDL2

NCBI Gene ID: 58205

Uniprot Entry: Q9WUL5

Construct Details: The recombinant mouse PD-L2-Fc fusion is expressed as a 438 amino acid protein consisting of Leu20 - Thr220 region of mouse PD-L2 (UniProt accession #Q9WUL5) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.

Source: Human cells stably expressing mouse PD-L2-Fc and growing in chemical-defined media with no animal components or antibiotics

Deduced Amino Acid Sequence:

10 20 30 40 50 60

FTVTAPKEVY TVDVGSSVSL ECDFDRRECT ELEGIRASLQ KVENDTSLQS ERATLLEEQL

70 80 90 100 110 120

PLGKALFHIP SVQVRDSGQY RCLVICGAAW DYKYLTVKVK ASYMRIDTRI LEVPGTGEVQ

130 140 150 160 170 180

LTCQARGYPL AEVSWQNVSV PANTSHIRTP EGLYQVTSVL RLKPQPSRNF SCMFWNAHMK

190 200 210 220 230 240

ELTSAIIDPL SRMEPKVPRT STTENLYFQG STGTHTCPPC PAPELLGGPS VFLFPPKPKD

250 260 270 280 290 300

TLMISRTPEV TCVVVDVSHE DPEVKFNWYV DGVEVHNAKT KPREEQYNST YRVVSVLTVL

310 320 330 340 350 360

HQDWLNGKEY KCKVSNKALP APIEKTISKA KGQPREPQVY TLPPSREEMT KNQVSLTCLV

370 380 390 400 410 420

KGFYPSDIAV EWESNGQPEN NYKTTPPVLD SDGSFFLYSK LTVDKSRWQQ GNVFSCSVMH

430

EALHNHYTQK SLSLSPGK

M.W.: Calculated molecular mass 49.2 kDa; estimated by SDS-PAGE under reducing condition ~60 kDa (probably due to glycosylation)

Calculated PI: 6.86

Calculated Extinction Coefficients (M-1 cm-1, at 280nm): 64580

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above)

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier and preservative free).

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

Biological Activity: Binds to its receptor PD-1 in a functional ELISA. Blocks the ligand PD-L1’s and PD-L2’s binding to its receptor and mediated signaling activity. Shows cross-species binding to human PD-1 (SKU# FCL0763B) with the highest affinity among all human and murine PD-1 ligands (see Technical Data).

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

Technical Data: Cross-species Interaction with Human PD-1 by ELISA

Recombinant mouse PD-L2-Fc (SKU#FCL1818) was immobilized at 100 ng/well on a 96-well NuncMaxisorp plate for a functional ELISA with serially diluted, biotinylated human PD-1-Fc (PD-1-Fc-Biot, SKU#FCL0763B) and biotinylated Fc alone (control Fc-Biot, SKU#FCL00029B). Biotinylated Fc protein alone was used as a negative control to demonstrate the specificity of PD-1:mPD-L2 interaction under the experimental conditions. Mouse PD-L2 shows cross-species reactivity to human PD-1 with the highest affinity/avidity among all human and murine PD-1 ligands. The binding curves were fit and apparent K_D was calculated for PD-1: mPD-L2 binding to be 126 ± 14 ng/ml with a linear binding range between 40 and 600 ng/ml.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

1. Nat. Immunol. 2: 261-268 (2001).

2. J. Exp. Med. 193: 839-846 (2001).

3. Proc. Natl. Acad. Sci. 100: 5336-5341 (2003).

4. Eur. J. Immunol. 35: 3561-3569 (2005).

5. Nature Immunol. 2:203 (2001).

6. Annu. Rev. Immunol. 20:29 (2002).

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Product datasheet (pdf) can be downloaded here: FCL1818-PDS.pdf