Human GITR Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant

Product ID: GITR-Fc (SKU#: FCL0289)

For Bulk Order: Call for price

Price:

$498.00

Description

GITR (Glucocorticoid-induced TNFR-related protein), also known as CD357 and AITR (Activation-inducible TNFR family receptor), is a single-pass type I transmembrane glycoprotein in the TNF receptor superfamily (TNFRSF18). GITR contains 3 TNFR cysteine-rich repeats in the extracellular region and a cytoplasmic domain that shows striking homology with those of TNFRSF members 41BB/CD137 and CD27. GITR is expressed at low levels in peripheral blood T cells, bone marrow, thymus, spleen, and lymph nodes. GITR is up regulated in peripherical mononuclear cells after antigen stimulation/lymphocyte activation by treatment with antiCD3 plus antiCD28, or PMA plus ionomycin. Knockout studies indicate that GITR may play a key role in dominant immunological self-tolerance maintained by CD25(+)CD4(+) regulatory T cells and in the regulation of CD3-driven T-cell activation and programmed cell death. GITR is the receptor for GITR ligand (GITRL) belonging to the TNF superfamily (TNFSF18). GITR and its ligand GITRL are involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Ligation of GITRL can activate NFκB through several TRAF adaptor proteins, and protect T cells from TCR activationinduced cell death. GITR and GITRL have also been shown to be important co-stimulatory molecules in the pathogenesis of autoimmune diseases.

Gene Symbol: TNFRSF18; AITR; CD357; GITR-D; UNQ319/PRO364

NCBI Gene ID: 8784

Uniprot Entry: Q9Y5U5

Construct Details: The recombinant human GITR-Fc fusion is expressed as a 364 amino acid protein consisting of Gln26 - Glu161 region of GITR (UniProt accession #Q9Y5U5) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.

Source: Human cells stably expressing human GITR-Fc and growing in chemical-defined media with no animal components or antibiotics

Deduced Amino Acid Sequence:

10 20 30 40 50 60

QRPTGGPGCG PGRLLLGTGT DARCCRVHTT RCCRDYPGEE CCSEWDCMCV QPEFHCGDPC

70 80 90 100 110 120

CTTCRHHPCP PGQGVQSQGK FSFGFQCIDC ASGTFSGGHE GHCKPWTDCT QFGFLTVFPG

130 140 150 160 170 180

NKTHNAVCVP GSPPAESTGT HTCPPCPAPE LLGGPSVFLF PPKPKDTLMI SRTPEVTCVV

190 200 210 220 230 240

VDVSHEDPEV KFNWYVDGVE VHNAKTKPRE EQYNSTYRVV SVLTVLHQDW LNGKEYKCKV

250 260 270 280 290 300

SNKALPAPIE KTISKAKGQP REPQVYTLPP SREEMTKNQV SLTCLVKGFY PSDIAVEWES

310 320 330 340 350 360

NGQPENNYKT TPPVLDSDGS FFLYSKLTVD KSRWQQGNVF SCSVMHEALH NHYTQKSLSL

SPGK

M.W.: Calculated molecular mass (kDa): 40.1; Estimated by SDS-PAGE under reducing condition (kDa): 45-50

Calculated PI: 6.72

Calculated extinction coefficients (M-1 cm-1, at 280nm): 49400

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "DTT: +")

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier & preservative free).

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

Biological Activity: Binds to its ligand GITRL/TNFSF18 and blocks GITRL-induced signaling activity.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

1. Proc. Natl. Acad. Sci. USA 94:6216 (1997).

2. J. Biol. Chem. 274:6056 (1999).

3. Current Biology 9:215 (1999).

4. Cell Death Differ. 7:408 (2000).

5. Oncol Rep. 18: 1189 (2007).

Product datasheet (pdf) can be downloaded here: FCL0289-PDS.pdf